Application of the detached-leaf technique to evaluate the pathogenicity of isolates of fungi Ascochyta fabae and Botrytis fabae to faba bean
Tomasz Góral
t.goral@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin PIB (Poland)
https://orcid.org/0000-0001-9130-6109
Abstract
The most important diseases of the faba bean include Ascochyta blight caused by the fungus Ascochyta fabae and chocolate spot caused by the fungus Botrytis fabae. The pathogenicity of isolates of A. fabae and B. fabae was investigated to six cultivars of faba bean. The technique of detached leaves was used. The leaves were inoculated with pycnidial and conidial spores of the pathogens tested. After incubation, the sizes of necrotic spots produced by pathogens were determined. Significant variation in the pathogenicity of isolates of both fungi was found. The varieties showed differential resistance to Ascochyta blight and chocolate spot. The variation in resistance of cultivars to chocolate spot was lower than resistance to Ascochyta blight. The possibility of using the ImageJ image analysis software to measure the surface of necrotic spots was described.
Keywords:
ascochyta blight, chocolate spot, Vicia faba, image analysis, faba bean, resistanceReferences
Abd-El-Haliem, A., 2012. An unbiased method for the quantitation of disease phenotypes using a custom-built macro plugin for the program ImageJ. In: Bolton, M., Thomma, B. (eds) Plant Fungal Pathogens. Methods in Molecular Biology, vol 835. Humana Press. https://doi.org/10.1007/978-1-61779-501-5_41
Google Scholar
Alheeti, A.A.M., Farhan, M.A., Al-Saad, L.A., Theer, R.M., 2021. Evaluation of the performance of ImageJ, Leaf Doctor applications, and visual assessments in measuring severity of two leaf spot diseases. IOP Conf. Ser. Earth Environ. Sci. 761. https://doi.org/10.1088/1755-1315/761/1/012030
Google Scholar
Avila, C.M., Satovic, Z., Sillero, J.C., Rubiales, D., Moreno, M.T., Torres, A.M., 2004. Isolate and organ-specific QTLs for ascochyta blight resistance in faba bean (Vicia faba L). Theor. Appl. Genet. 108, 1071–1078. https://doi.org/10.1007/s00122-003-1514-7
Google Scholar
Avila, C.M., Atienza, S.G., Moreno, M.T., Torres, A.M., 2007. Development of a new diagnostic marker for growth habit selection in faba bean (Vicia faba L.) breeding. Theor. Appl. Genet. 115, 1075–1082. https://doi.org/10.1007/s00122-007-0633-y
Google Scholar
Bartosiak, S., 2020. Automatyzacja oceny objawów chorobowych septorioz zbóż z wykorzystaniem komputerowej analizy obrazu w języku programowania Python. Biul. Inst. Hod. i Aklim. Roślin 31–35. https://doi.org/10.37317/BIUL-2020-0019
Google Scholar
Blake, S.N., Lee, R.C., Russ, M.H., Farquharson, E.A., Rose, J.A., Herdina, Goonetilleke, S.N., Farfan-Caceres, L.M., Debler, J.W., Syme, R.A., Davidson, J.A., 2022. Phenotypic and Genotypic Diversity of Ascochyta fabae Populations in Southern Australia. Front. Plant Sci. 13, 2185. https://doi.org/10.3389/FPLS.2022.918211/BIBTEX
Google Scholar
Bond, D.A., Jellis, G.J., Rowland, G.G., Le Guen, J., Robertson, L.D., Khalil, S.A., Li-Juan, L., 1994. Present status and future strategy in breeding faba beans (Vicia faba L.) for resistance to biotic and abiotic stresses, w: Muehlbauer, F.J., Kaiser, W.J. (Red.), Expanding the Production and Use of Cool Season Food Legumes. Current Plant Science and Biotechnology in Agriculture, vol 19. Springer, Dordrecht, Dodrecht, ss. 592–616. https://doi.org/10.1007/978-94-011-0798-3_36
Google Scholar
Boros L., Góral T., Walentyn-Góral D., Wawer A., Borucka K. 2014. Śledzenie zmian patogeniczności w populacjach ważnych gospodarczo grzybów roślin strączkowych (Mycosphaerella pinodes, Ascochyta fabae, Botrytis fabae, Fusarium sp.) – sprawców zgorzelowej plamistości grochu i bobiku. (W:) Ulepszanie Roślin dla Zrównoważonych AgroEkoSystemów, Wysokiej Jakości Żywności i Produkcji Roślinnej na Cele Nieżywnościowe. Monografie i Rozprawy Naukowe IHAR. Red. Edward Arseniuk, Nr 48: 313-322. (całość 408 s.).
Google Scholar
Bouhassan, A., Sadiki, M., Tivoli, B., 2004. Evaluation of a collection of faba bean (Vicia faba L.) genotypes originating from the Maghreb for resistance to chocolate spot (Botrytis fabae) by assessment in the field and laboratory. Euphytica 135, 55–62. https://doi.org/10.1023/B:EUPH.0000009540.98531.4d
Google Scholar
Díaz-Ruiz, R., Satovic, Z., Ávila, C.M., Alfaro, C.M., Gutierrez, M. V., Torres, A.M., Román, B., 2009. Confirmation of QTLs controlling Ascochyta fabae resistance in different generations of faba bean (Vicia faba L.). Crop Pasture Sci. 60, 353–361. https://doi.org/10.1071/CP08190
Google Scholar
Elliott, K., Berry, J.C., Kim, H., Bart, R.S., 2022. A comparison of ImageJ and machine learning based image analysis methods to measure cassava bacterial blight disease severity. Plant Methods 18, 1–12. https://doi.org/10.1186/s13007-022-00906-x
Google Scholar
Fouad, M., Mohammed, N., Aladdin, H., Ahmed, A., Xuxiao, Z., Shiying, B., Tao, Y., 2013. Faba Bean, w: Singh, M., Upadhyaya, H.D., Bisht, I.S. (Red.), Genetic and Genomic Resources of Grain Legume Improvement. Elsevier, London, ss. 113–136. https://doi.org/10.1016/B978-0-12-397935-3.00005-0
Google Scholar
Gela, T.S., Bruce, M., Chang, W., Stoddard, F.L., Schulman, A.H., Vandenberg, A., Khazaei, H., 2022. Genomic regions associated with chocolate spot (Botrytis fabae Sard.) resistance in faba bean (Vicia faba L.). Mol. Breed. 42, 1–10. https://doi.org/10.1007/S11032-022-01307-7/FIGURES/3
Google Scholar
Góral T, Walentyn-Góral D. 2012. Using detached leaf technique for assessment of pathogenicity of Ascochyta fabae Speg. isolates to faba bean (Vicia faba L.). Proceedings Book of the IIIrd International Ascochyta Workshop”, 22-26 April. Cordoba, Spain, p. 84.
Google Scholar
Grela, E.R., Czech, A., 2019. Pasze alternatywne w odniesieniu do soi genetycznie modyfikowanej w żywieniu zwierząt. Wiadomości Zootech. 2, 66–77.
Google Scholar
Hanounik, S.B., Robertson, L.D., 1989. Resistance in Vicia faba germ plasm to blight caused by Ascochyta fabae. Plant Dis. 73, 202. https://doi.org/10.1094/PD-73-0202
Google Scholar
Hartmann, A., Czauderna, T., Hoffmann, R., Stein, N., Schreiber, F., 2011. HTPheno: An image analysis pipeline for high-throughput plant phenotyping. BMC Bioinformatics 12. https://doi.org/10.1186/1471-2105-12-148
Google Scholar
Herath, I.H.M.H.B., Stoddard, F.L., Marshall, D.R., 2001. Evaluating faba beans for rust resistance using detached leaves. Euphytica 117, 47–57. https://doi.org/10.1023/A:1004071916288
Google Scholar
Jellis, G.J., Lockwood, G., Aubury, R.G., 1985. Phenotypic influences on the incidence of infection by Ascochyta fabae in spring varieteies of faba beans (Vicia faba). Plant Pathol. 34, 347–352. https://doi.org/10.1111/j.1365-3059.1985.tb01371.x
Google Scholar
Kaiser, W.J., Wang, B.C., Rogers, J.D., 2007. Ascochyta fabae and A. lentis: Host Specificity, Teleomorphs (Didymella), Hybrid Analysis, and Taxonomic Status. Plant Dis. 81, 809–816. https://doi.org/10.1094/PDIS.1997.81.7.809
Google Scholar
Kaur, S., Kimber, R.B.E., Cogan, N.O.I., Materne, M., Forster, J.W., Paull, J.G., 2014. SNP discovery and high-density genetic mapping in faba bean (Vicia faba L.) permits identification of QTLs for ascochyta blight resistance. Plant Sci. 217–218, 47–55. https://doi.org/10.1016/J.PLANTSCI.2013.11.014
Google Scholar
Kharrat, M., Le Guen, J., Tivoli, B., 2006. Genetics of resistance to 3 isolates of Ascochyta fabae on Faba bean (Vicia faba L.) in controlled conditions. Euphytica 151, 49–61. https://doi.org/10.1007/s10681-006-9127-2
Google Scholar
Kohpina, S., Knight, R., Stoddard, F.L., 2000a. Genetics of resistance to ascochyta blight in two populations of faba bean. Euphytica 112, 101–107. https://doi.org/10.1023/A:1003853126862
Google Scholar
Kohpina, S., Knight, R., Stoddard, F.L., 2000b. Evaluating faba beans for resistance to ascochyta blight using detached organs. Aust. J. Exp. Agric. 40, 707–713. https://doi.org/10.1071/EA99168
Google Scholar
Lendenmann, M.H., Croll, D., Stewart, E.L., McDonald, B.A., 2014. Quantitative trait locus mapping of melanization in the plant pathogenic fungus Zymoseptoria tritici. G3 Genes, Genomes, Genet. 4, 2519–2533. https://doi.org/10.1534/g3.114.015289
Google Scholar
Maalouf, F., Ahmed, S., Shaaban, K., Bassam, B., Nawar, F., Singh, M., Amri, A., 2016. New faba bean germplasm with multiple resistances to Ascochyta blight, chocolate spot and rust diseases. Euphytica 211, 157–167. https://doi.org/10.1007/S10681-016-1726-Y/TABLES/6
Google Scholar
Mutka, A.M., Bart, R.S., Avni, A., Tör, M., Danforth, D., 2015. Image-based phenotyping of plant disease symptoms. https://doi.org/10.3389/fpls.2014.00734
Google Scholar
Ondřej, M., Huňady, I., 2007. Faba bean (Vicia faba L.) breeding for resistance to anthracnose (Ascochyta fabae Speg.) in the Czech Republic. Czech J. Genet. Plant Breed. 43, 61–68.
Google Scholar
Ozkilinc, H., Thomas, K., Abang, M., Peever, T.L., 2015. Population structure and reproductive mode of Didymella fabae in Syria. Plant Pathol. 64, 1110–1119. https://doi.org/10.1111/ppa.12359
Google Scholar
Panek J. 2022. Co uprawia się w Polsce? Struktura zasiewów 2022. https://terazpole.pl/wokol-pola/co-uprawia-sie-w-polsce-struktura-zasiewow-2022/
Google Scholar
Pethybridge, S.J., Nelson, S.C., 2015. Leaf doctor: A new portable application for quantifying plant disease severity. Plant Dis. 99, 1310–1316. https://doi.org/10.1094/PDIS-03-15-0319-RE
Google Scholar
Pritchard, P.R., Rowe, P.S., Rossall, S., 1989. A comparison of infection of resistant and susceptible lines of field bean (Vicia faba) by Ascochyta fabae. Plant Pathol. 38, 266–270. https://doi.org/10.1111/j.1365-3059.1989.tb02142.x
Google Scholar
Román, B., Satovic, Z., Avila, C.M., Rubiales, D., Moreno, M.T., Torres, A.M., 2003. Locating genes associated with Ascochyta fabae resistance in Vicia faba. Aust. J. Agric. Res. 54, 85–90. https://doi.org/10.1071/AR02034
Google Scholar
Rubiales, D., Trapero-Casas, A., 2002. Occurrence of Didymella fabae, the Teleomorph of Ascochyta fabae, on Faba Bean Straw in Spain. J. Phytopathol. 150, 1–3.
Google Scholar
Schneider, C. A., Rasband, W. S., Eliceiri, K. W., 2012. NIH Image to ImageJ: 25 years of image analysis. Nature Methods 9(7), 671–675. https://doi.org/10.1038/nmeth.2089
Google Scholar
Sillero, J.C., Villegas-Fernández, A.M., Thomas, J., Rojas-Molina, M.M., Emeran, A. A., Fernández-Aparicio, M., Rubiales, D., 2010. Faba bean breeding for disease resistance. F. Crop. Res. 115, 297–307. https://doi.org/10.1016/j.fcr.2009.09.012
Google Scholar
Stewart, E.L., Hagerty, C.H., Mikaberidze, A., Mundt, C.C., Zhong, Z., McDonald, B.A., 2016. An improved method for measuring quantitative resistance to the wheat pathogen Zymoseptoria tritici using high-throughput automated image analysis. Phytopathology 106, 782–788. https://doi.org/10.1094/PHYTO-01-16-0018-R
Google Scholar
Tivoli, B., Baranger, A., Avila, C.M., Banniza, S., Barbetti, M., Chen, W., Davidson, J., Lindeck, K., Kharrat, M., Rubiales, D., Sadiki, M., Sillero, J.C., Sweetingham, M., Muehlbauer, F.J., 2006. Screening techniques and sources of resistance to foliar diseases caused by major necrotrophic fungi in grain legumes. Euphytica 147, 223–253. https://doi.org/10.1007/s10681-006-3131-4
Google Scholar
Villegas-Fernández, A. M., Sillero, J.C., Rubiales, D., 2011. Screening faba bean for chocolate spot resistance: evaluation methods and effects of age of host tissue and temperature. Eur. J. Plant Pathol. 132, 443–453. https://doi.org/10.1007/s10658-011-9889-9
Google Scholar
Walentyn-Góral D., Góral T. 2011. Ocena patogeniczności izolatów Ascochyta fabae Speg. wobec bobiku (Vicia faba L.) metodą odciętych liści [Evaluation of pathogenicity of Ascochyta fabae Speg. isolates to faba bean (Vicia faba L.) using detached-leaf technique]. Materiały Sympozjum Naukowego “Fitopatologia: zdrowe rośliny – zdrowi ludzie”, Bydgoszcz, 20-22 września 2011, str. 416-417.
Google Scholar
Zaki, K.I., 2010. Evaluation of Some Faba Bean Genotypes for Resistance to chocolate spot. Egypt. J. Phythopathology 38, 25–43.
Google Scholar
Zakrzewska, E., 2004. Reaction of morphological types of faba bean to infection with Ascochyta fabae Speg. and Botrytis fabae Sard. Plant Breed. Seed Sci. 49, 3–17.
Google Scholar
Zakrzewska, E., 1988. Variability in the resistance of Vicia faba L. to Ascochyta fabae Speg. Hod. Roślin, Aklim. i Nasienn. 32, 311–317.
Google Scholar
Authors
Tomasz Góralt.goral@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin PIB Poland
https://orcid.org/0000-0001-9130-6109
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