Dumas method as an alternative approach for determining protein contents of plant products — comparative study with Kjeldahl method
Kinga Myszka
k.myszka@ihar.edu.plSamodzielna Pracownia Oceny Jakości Produktów Roślinnych, Instytut Hodowli i Aklimatyzacji Roślin — PIB w Radzikowie (Poland)
Beata Kamińska
Samodzielna Pracownia Oceny Jakości Produktów Roślinnych, Instytut Hodowli i Aklimatyzacji Roślin — PIB w Radzikowie (Poland)
Anna Fraś
Samodzielna Pracownia Oceny Jakości Produktów Roślinnych, Instytut Hodowli i Aklimatyzacji Roślin — PIB w Radzikowie (Poland)
Magdalena Ploch
Samodzielna Pracownia Oceny Jakości Produktów Roślinnych, Instytut Hodowli i Aklimatyzacji Roślin — PIB w Radzikowie (Poland)
Danuta Boros
Samodzielna Pracownia Oceny Jakości Produktów Roślinnych, Instytut Hodowli i Aklimatyzacji Roślin — PIB w Radzikowie (Poland)
Abstract
Increased qualitative demand for the knowledge of protein contents in various plant products as well in foods and feeds has led to wider used of the Dumas method, which is even older technique than commonly employed Kjeldahl procedure. Aim of the study was to compare the results of protein contents of 138 samples of cereal grains, such as wheat, barley and oat and 5 samples of meals produced from rapeseed and soybean in Polish agricultural conditions, obtained by the Kjeldahl and Dumas methods. Very high correlation coefficient was obtained between protein contents analysed with the Dumas and Kjeldahl methods, equal to 0.9983 for the entire material tested. However, the results obtained by Dumas method were, on average, by 0.46 percentage units higher than those analysed with Kjeldahl procedure. The differences between methods applied depended on the materials to be analysed. These results suggest that Dumas method can be successfully used as an alternative method for testing content of protein in cereal grains and rapeseed and soybean meals.
Keywords:
cereal grains, Dumas method, Kjeldahl method, rapeseed meal, soybean meal, total nitrogenReferences
Agencja Rynku Rolnego. http://www.arr.gov.pl
Google Scholar
AOAC. 1995. Official methods of analysis of the association of official analytical chemists, 16th ed. AOAC, Arlington, method 990.03 (Dumas).
Google Scholar
AOAC. 1995. Official methods of analysis of the association of official analytical chemists, 16th ed. AOAC, Arlington, method 925.10 (Kjeldahl).
Google Scholar
Baca E., Gołębiewski T. 1997. Nowe spojrzenie na wskaźniki warunkujące wartość technologiczną jęczmienia i słodu browarnego. Przem. Ferm. Owoc. Warz. 10: 18 — 22.
Google Scholar
Beljkaš B., Matić J., Milovanović I., Jovanov P., Mišan A., Šarić L. 2010. Rapid method for determination of protein content in cereals and oilseeds: validation, measurement uncertainty and comparison with the Kjeldahl method. Accred. Qual. Assur. 15: 555 — 561.
Google Scholar
Dumas, J. B. A. 1831. Procedes de l´analyse organic , Ann. Chim. Phys. 247: 198 — 213.
Google Scholar
Daun J.K., DeClercq D.R.. 1994. Comparison of combustion and Kjeldahl methods for determination of nitrogen in oilseeds. J. Amer. Oil Chem. Soc. 71 (10): 1069 — 1072.
Google Scholar
Gąsiorowski H. (red.). 2004. Pszenica. Chemia i Technologia. PWRiL, Poznań.
Google Scholar
Gizzi G., Givens D. I. 2004. Variability in feed composition and its impact on animal production. In: Assessing quality and safety of animal feeds. FAO Animal production and health, nr 160: 39 — 54.
Google Scholar
Hanczakowski P. 2001. Rośliny jako źródła białka. Podrozdział „Składniki pokarmowe i antyodżywcze występujące w roślinach” autor.: Honczakowski, Koreleski i Wolski. Monografia wydana przez Instytut Zootechniki, Kraków — Balice: 11 — 19.
Google Scholar
Jung S., Rickert D. A., Deak N. A., Aldin E. D., Recknor J., Johnson L. A., Murphy P. A. 2003. Comparison of Kjeldahl and Dumas methods for determining protein contents of soybean products. J. Am. Oil Chem. Soc. 80 (12): 1169 — 1173.
Google Scholar
Kjeldahl J. Z. 1883. A new method for the determination of nitrogen in organic bodies. Analytical Chemistry 22: 366 — 382.
Google Scholar
Klockiewicz-Kamińska E. 2005. Metody oceny wartości browarnej i klasyfikacja jakościowa odmian jęczmienia. COBORU Słupia Wielka.
Google Scholar
Krotz L, Cicerci E, Giazzi G. 2008. Protein determination in cereals and seeds. Food Quality 15 (4): 37–39.
Google Scholar
Leeson S., Atteh J. O., Summers J. D. 1987. The replacement value of canola meal for soybean meal in poultry diets. Can J. Anim. Sci. 65: 151 — 158.
Google Scholar
Marco A., Rubio R., Compano R., Casals I. 2002. Comparison of the Kjeldahl method and a combustion method for total nitrogen determination in animal feed. Talanta 57 (5): 1019 — 1026.
Google Scholar
Meller E. L., Bimbo A. P., Barlow S. M. Sheridan B. 2007. Repeatability and reproducibility of determination of the nitrogen content of fishmeal by the combustion (Dumas) method and comparison with the Kjeldahl method: Interlaboratory study. J. AOAC International 90 (1): 6 — 21.
Google Scholar
Möller J. 2009. Kjeldahl — still going strong. In: Focus 33: 14 — 16.
Google Scholar
Normy żywienia drobiu. 1996. Praca zbiorowa pod red. S. Smulikowskiej. Wyd. IFŻZ im. J. Kielanowskiego PAN, Jabłona.
Google Scholar
Rakowska M. 1978. Propozycje standaryzacji metod analitycznych i biologicznych do oceny materiału roślinnego w pracach nad selekcją nowych odmian roślin uprawnych o podwyższonej zawartości i jakości białka. Biul. IHAR 133: 97 — 114.
Google Scholar
Saint-Denis T., Goupy J. 2004. Optimization of a nitrogen analyser based on the Dumas method. Analytica Chimica Acta 515(1): 191 — 198.
Google Scholar
Simonne AH, Simonne EH, Eitenmiller RR, Mills HA, Cresman III CP.1997. Could the Dumas method replace the Kjeldahl digestion for nitrogen and crude protein determinations in foods? J. Sci. Food Agric. 73:39–45.
Google Scholar
Shewry P. R., 2007. Improving the protein content and composition of cereal grain. J. Cereal Sci. 46: 239–250.
Google Scholar
Watson M. E., Galliher T. L. 2001. Comparison of Dumas and Kjeldahl methods with automatic analyser on agricultural samples under routine rapid analysis conditions. Commun. Soil Sci. Plant Anal. 32: 2007 — 2019.
Google Scholar
Wiles P. G., Gray I. K., Kissling R. C. 1998. Routine analysis of proteins by Kjeldahl and Dumas methods: review and interlaboratory study using dairy products. J. AOAC International, 81: 620 — 632.
Google Scholar
Williams P., Sobering D., Antoniszyn J. 1998. Protein testing methods. In: Fowler D.B., Geddes W.E., Johnston A.M, Preston K.R. (eds.) Wheat protein production and marketing. Proc. of the Wheat Protein Symposium. 9–10 March 1998.Univ. Extension Press. Univ. of Saskatchewan, Saskatoon, Canada: 37 — 47.
Google Scholar
Authors
Kinga Myszkak.myszka@ihar.edu.pl
Samodzielna Pracownia Oceny Jakości Produktów Roślinnych, Instytut Hodowli i Aklimatyzacji Roślin — PIB w Radzikowie Poland
Authors
Beata KamińskaSamodzielna Pracownia Oceny Jakości Produktów Roślinnych, Instytut Hodowli i Aklimatyzacji Roślin — PIB w Radzikowie Poland
Authors
Anna FraśSamodzielna Pracownia Oceny Jakości Produktów Roślinnych, Instytut Hodowli i Aklimatyzacji Roślin — PIB w Radzikowie Poland
Authors
Magdalena PlochSamodzielna Pracownia Oceny Jakości Produktów Roślinnych, Instytut Hodowli i Aklimatyzacji Roślin — PIB w Radzikowie Poland
Authors
Danuta BorosSamodzielna Pracownia Oceny Jakości Produktów Roślinnych, Instytut Hodowli i Aklimatyzacji Roślin — PIB w Radzikowie Poland
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Copyright (c) 2011 Kinga Myszka, Beata Kamińska, Anna Fraś, Magdalena Ploch, Danuta Boros
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