Fuzarioza kłosów oraz akumulacja toksyn fuzaryjnych w ziarnie rodów hodowlanych pszenicy ozimej
Fusarium head blight and Fusarium toxins accumulation in grain of breeding lines of winter wheat
Tomasz Góral
t.goral@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin - Państwowy Instytut Badawczy (Poland)
Halina Wiśniewska
Zakład Genomiki, Instytut Genetyki Roślin PAN (Poland)
Piotr Ochodzki
Instytut Hodowli i Aklimatyzacji Roślin - Państwowy Instytut Badawczy (Poland)
Dorota Walentyn-Góral
Instytut Hodowli i Aklimatyzacji Roślin - Państwowy Instytut Badawczy (Poland)
Iga Grzeszczak
Instytut Hodowli i Aklimatyzacji Roślin - Państwowy Instytut Badawczy (Poland)
Jolanta Belter
Zakład Genomiki, Instytut Genetyki Roślin PAN (Poland)
Maciej Majka
Zakład Genomiki, Instytut Genetyki Roślin PAN (Poland)
Jerzy Bogacki
DANKO Hodowla Roślin (Poland)
Tadeusz Drzazga
Małopolska Hodowla Roślin HBP (Poland)
Bogusława Ługowska
DANKO Hodowla Roślin (Poland)
Przemysław Matysik
Hodowla Roślin Strzelce Grupa IHAR Sp. z o.o. (Poland)
Edward Witkowski
Hodowla Roślin Smolice Sp. z o.o. Grupa IHAR
Krzysztof Rubrycki
Poznańska Hodowla Roślin (Poland)
Urszula Woźna-Pawlak
Poznańska Hodowla Roślin (Poland)
Abstract
Resistance to Fusarium head blight of wheat breeding lines was tested under field conditions in IHAR-PIB Radzików and IGR PAN Poznań (experimental field in Cerekwica). Heads were inoculated with isolates of Fusarium culmorum. Resistance to Fusarium head blight (resistance type I + II) of most of the lines from the collection of resistant forms was confirmed. High correlation coefficients (0.775–0.613) of FHB indexes for the genotypes tested in 2014–2016 were found. There was no significant relationship between resistance type I and type II. These resistances poorly correlated with the FHB indexes in field experiments, higher correlation coefficients were found for FHB indexes vs average resistance of both types. The tested lines showed varied resistance to the Fusarium kernel damage (type III). The FHB index significantly correlated with kernel damage. Fusarium head blight caused a reduction in yield of grain from head (type IV). A significant correlation of FHB index and kernel damage to grain yield reduction was found. Lines combining the types of resistance I + II, III and IV were identified. The tested lines showed varied resistance to the accumulation of Fusarium toxins (trichothecenes B and zearalenone) in the grain (type V). Wheat lines showing resistance of type V were identified. FHB index, Fusarium kernel damage and ergosterol content (measure of mycelium content in the grain) correlated highly significantly with the contents of the trichothecenes B in the grain. Correlation of FHB index, kernel damage and ergosterol content was weaker for zearalenone. Lines combining increased resistance of all studied types were identified.
Keywords:
Fusarium culmorum, Fusarium head blight, trichothecenes, wheat, zearalenoneReferences
Argyris J., Van Sanford D., TeKrony D. 2003. Fusarium graminearum infection during wheat seed development and its effect on seed quality. Crop Sci. 43:1782 — 1788.
Google Scholar
Bechtel D. B., Kaleikau L. A., Gaines R. L., Seitz L. M. 1985. The effects of Fusarium graminearum infection on wheat kernels. Cereal Chem. 62: 191 — 197.
Google Scholar
Bottalico A., Perrone G. 2002. Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. Eur. J. Plant Pathol. 108: 998 — 1003.
Google Scholar
Bottalico A. 1998. Fusarium diseases of cereals: Species complex and related mycotoxin profiles, in Europe. J. Plant Pathol. 80: 85 — 103.
Google Scholar
Buerstmayr H., Ban T., Anderson J. A. 2009. QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: A review. Plant Breed. 128: 1 — 26.
Google Scholar
Buerstmayr H., Lemmens M., Berlakovich S., Ruckenbauer P. 1999. Combining ability of resistance to head blight caused by Fusarium culmorum (W.G. Smith) in the F1 of a seven parent dialler of winter wheat (Triticum aestivum L.). Euphytica 110: 199 — 206.
Google Scholar
Buerstmayr H., Lemmens M., Hartl L., Doldi L., Steiner B., Stierschneider M., Ruckenbauer P. 2002. Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (Type II resistance). Theor. Appl. Genet. 104: 84 — 91.
Google Scholar
Buerstmayr H., Steiner B., Hartl L., Griesser M., Angerer N., Lengauer D., Miedaner T., Schneider B., Lemmens M. 2003. Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread. Theor. Appl. Genet. 107: 503 — 508.
Google Scholar
Buerstmayr H., Lemmens M., Schmolke M., Zimmermann G., Hartl L., Mascher F., Trottet M., Gosman N. E., Nicholson P. 2008. Multi-environment evaluation of level and stability of FHB resistance among parental lines and selected offspring derived from several European winter wheat mapping populations. Plant Breeding 127: 325 — 332.
Google Scholar
Burlakoti R. R., Mergoum M., Kianian S.F., Adhikari T. B. 2010. Combining different resistance components enhances resistance to Fusarium head blight in spring wheat. Euphytica 172: 197 — 205.
Google Scholar
Bushnell W. R. 2001. What is known about infection pathways in Fusarium head blight? In S. Canty et al. (ed.) Proceedings of the 2001 National Fusarium Head Blight Forum. Kinko's, Okemos, MI, USA, p.: 105.
Google Scholar
Chelkowski J. 1998. Distribution of Fusarium species and their mycotoxins in cereal grains. In: Sinha, K. K., Bhatnagar, D. (Eds.), Mycotoxins in Agriculture and Food Safety. Marcel Dekker, Inc., New York-Basel-Hong Kong: 45 — 64.
Google Scholar
Clark B., Jorgensen L. N., Antichi D., Góral T., Gouache D., Hornok L., Jahn M., Lucas P., Rolland B., Schepers H. 2009. Strategies to control Fusarium ear blight and mycotoxin production in wheat. From Science to Field. Wheat Case Study — Guide Number 2. ENDURE [http://www.edndure-network.eu].
Google Scholar
Cowger C., Arellano C. 2013. Fusarium graminearum infection and deoxynivalenol concentrations during development of wheat spikes. Phytopathology 103: 460 — 471.
Google Scholar
Cuthbert P. A., Somers D. J., Thomas J., Cloutier S., Brulé-Babel A. 2006. Fine mapping Fhb1, a major gene controlling Fusarium head blight resistance in bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 112: 1465 — 1472.
Google Scholar
Cuthbert P. A., Somers D. J., Brulé-Babel A. 2007. Mapping of Fhb2 on chromosome 6BS: a gene controlling Fusarium head blight field resistance in bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 114: 429 — 37.
Google Scholar
Döll S., Dänicke S. 2011. The Fusarium toxins deoxynivalenol (DON) and zearalenone (ZON) in animal feeding. Prev. Vet. Med. 102: 132 — 145.
Google Scholar
Foroud N. A., Eudes F. 2009. Trichothecenes in cereal grains. Int. J. Mol. Sci. 10: 147 — 173.
Google Scholar
Góral T. 2006. Odporność odmian pszenicy ozimej na fuzariozę kłosów powodowaną przez Fusarium culmorum (W. G. Smith) Sacc. Biul. IHAR 242: 63 — 78.
Google Scholar
Góral T., Stuper-Szablewska, K., Buśko, M., Boczkowska, M., Walentyn-Góral, D., Wiśniewska, H., Perkowski, J. 2015. Relationships between genetic diversity and fusarium toxin profiles of winter wheat cultivars. Plant Pathology J. 31: 226 — 244.
Google Scholar
Góral T., Wiśniewska H., Ochodzki P., Walentyn-Góral D., Kwiatek M. 2013. Reaction of winter triticale breeding lines to Fusarium head blight and accumulation of Fusarium metabolites in grain in two environments under drought conditions. Cereal Res. Commun. 41: 106 — 115.
Google Scholar
Góral T., Wiśniewska H., Walentyn-Góral D., Radecka-Janusik M., Czembor P. 2016. Resistance to Fusarium head blight [Fusarium culmorum (W.G. Sm.) Sacc.] of winter wheat lines generated from crosses between winter type cultivars and resistant spring wheat Sumai 3. Prog. Plant Prot. 56: 285 — 295.
Google Scholar
Gunnaiah R., Kushalappa A. C., Duggavathi R., Fox S., Somers D. J. 2012. Integrated metabolo-proteomic approach to decipher the mechanisms by which wheat QTL (Fhb1) contributes to resistance against Fusarium graminearum. PLoS One 7: e40695.
Google Scholar
Jennings P., Coates M. E., Turner J.A., Chandler E. A., Nicholson P. 2004. Determination of deoxynivalenol and nivalenol chemotypes of Fusarium culmorum isolates from England and Wales by PCR assay. Plant Path. 53: 182 — 190.
Google Scholar
Jones R. K., Mirocha C. J. 1999. Quality parameters in small grains from Minnesota affected by Fusarium head blight. Plant Dis. 83: 506 — 511.
Google Scholar
Kubo K., Kawada N., Fujita M. 2013b. Evaluation of Fusarium head blight resistance in wheat and the development of a new variety by integrating type I and II resistance. Jircas. Affrc. Go. Jp. 47: 9 — 19.
Google Scholar
Lemmens M., Buerstmayr H., Krska, R., Schuhmacher R., Grausgruber H., Ruckenbauer P. 2004. The effect of inoculation treatment and long-term application of moisture on Fusarium head blight symptoms and deoxynivalenol contamination in wheat grains. Eur. J. Plant Pathol. 110: 299 — 308.
Google Scholar
Lemmens M., Koutnik A., Steiner B., Buerstmayr H., Berthiller F., Schuhmacher R., Maier F., Schäfer W. 2008. Investigations on the ability of Fhb1 to protect wheat against nivalenol and deoxynivalenol. Cereal Res. Commun. 36: 429 — 435
Google Scholar
Logrieco A. 2001. Occurrence of toxigenic fungi and related mycotoxins in plants, food and feed in Europe. COST-835. European Commission, Brussels.
Google Scholar
McMullen M. P., Enz J., Lukach J., Stover R. 1997. Environmental conditions associated with Fusarium head blight epidemics of wheat and barley in the Northern Great Plains, North America. Cereal Res. Commun. 25: 777 — 778.
Google Scholar
Mesterhazy A. 1995. Types and components of resistance to Fusarium head blight of wheat. Plant Breeding 114: 377 — 386.
Google Scholar
Mesterhazy A. 2002. Theory and practice of the breeding for Fusarium head blight resistance in wheat. J. Appl. Genet. 43A: 289 — 302.
Google Scholar
Mesterházy A., Tóth B., Bartók T., Varga M. 2008. Breeding strategies against FHB in winter wheat and their relation to type I resistance. Cereal Res. Commun. 36: 37 — 43.
Google Scholar
Mesterhazy A., Toth B., Kaszonyi G. 2006. Sources of “environmental interactions” in phenotyping and resistance evaluation; Ways to neutralize them. In: Ban T., Lewis J.M., Phipps E.E. (Eds.), The global Fusarium initiative for international collaboration — strategic planning workshop held at CIMMYT: El Batan, Mexico, 14–17 March 2006: 84 — 92.
Google Scholar
Miedaner T., Moldovan M., Ittu M. 2003. Comparison of spray and point inoculation to assess resistance to Fusarium head blight in multienvironment wheat trial. Phytopathology 93: 1068 — 1072.
Google Scholar
Miedaner T., Voss H.-H. 2008. Effect of dwarfing genes on Fusarium head blight resistance in two sets of near-isogenic lines of wheat and check cultivars. Crop Sci. 48: 2115 — 2122.
Google Scholar
Minervini F., Dell’Aquila M. E. 2008. Zearalenone and reproductive function in farm animals. Intern. J. Molec. Sci. 90: 2570 — 2584.
Google Scholar
Musa T., Hecker A., Vogelgsang S., Forrer H. R. 2007. Forecasting of Fusarium head blight and deoxynivalenol content in winter wheat with FusaProg. EPPO Bulletin 37: 283 — 289.
Google Scholar
Neuhof T., Koch M., Rasenko T., Nehls I. 2008. Distribution of trichothecenes, zearalenone, and ergosterol in a fractionated wheat harvest lot. Journal of Agric. Food Chem. 56: 7566 — 7571.
Google Scholar
Paillard S., Schnurbusch T., Tiwari R., Messmer M., Winzeler M., Keller B., Schachermayr G. 2004. QTL analysis of resistance to Fusarium head blight in Swiss winter wheat (Triticum aestivum L.). Theor. Appl. Genet. 109: 323 — 332.
Google Scholar
Perkowski J., Buśko M., Stuper K., Kostecki M., Matysiak A., Szwajkowska-Michałek L. 2008. Concentration of ergosterol in small-grained naturally contaminated and inoculated cereals. Biologia. 63: 542 — 547.
Google Scholar
Perkowski J., Wiwart M., Buśko M., Laskowska M., Berthiller A., Kandler S., Krska R. 2007. Fusarium toxins and total fungal biomass indicators in naturally contaminated wheat samples from north-eastern Poland in 2003. Food Additiv Contam. 24 (11): 1292 — 1298.
Google Scholar
Pestka J. J. 2008. Mechanisms of deoxynivalenol-induced gene expression and apoptosis. Food Addit. Contam. — Part A Chem. Anal. Control. Expo. Risk Assess. 25: 1128 — 1140.
Google Scholar
Tamburic-Ilincic L., Falk, D. E., Schaafsma A. 2011. Fusarium ratings in Ontario Winter Wheat Performance Trial (OWWPT) using an index that combines Fusarium head blight symptoms and deoxynivalenol levels. Czech J. Genet. Plant Breed. 47: 115 — 122.
Google Scholar
Tomczak M., Wiśniewska H., Stępień Å., Kostecki M., Chełkowski J., Goliński P. 2002. Deoxynivalenol, nivalenol and moniliformin in wheat samples with head blight (scab) symptoms in Poland (1998–2000). Eur. J. Plant Pathol. 108: 625 — 630.
Google Scholar
van der Fels-Klerx H. J., Olesen J. E., Madsen M. S., Goedhart P. W. 2012. Climate change increases deoxynivalenol contamination of wheat in north-western Europe. Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 29: 1593 — 604.
Google Scholar
Wiśniewska H., Perkowski J. Kaczmarek Z. 2004. Scab response and deoxynivalenol accumulation in spring wheat kernels of different geographical origins following inoculation with Fusarium culmorum. J. Phytopathology 152: 613 — 621.
Google Scholar
Xu, X.-M., Monger W., Ritieni A., Nicholson P. 2007. Effect of temperature and duration of wetness during initial infection periods on disease development, fungal biomass and mycotoxin concentrations on wheat inoculated with single, or combinations of, Fusarium species. Plant Pathol. 56: 943 — 956.
Google Scholar
Xue S., Li, G., Jia H., Xu F., Lin F., Tang M., Wang Y., An X., Xu H., Zhang L., Kong Z., Ma Z. 2010. Fine mapping Fhb4, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 121: 147 — 156.
Google Scholar
Xue S., Xu F., Tang M., Zhou Y., Li G., An X., Lin F., Xu H., Jia H., Zhang L., Kong Z., Ma Z. 2011. Precise mapping Fhb5, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 123: 1055 — 1063.
Google Scholar
Yan W., Li H .B., Cai S. B., Ma H. X., Rebetzke G. J., Liu C. J. 2011. Effects of plant height on type I and type II resistance to Fusarium head blight in wheat. Plant Pathology 60: 506 — 512.
Google Scholar
Yazar S., Omurtag G. Z. 2008. Fumonisins, trichothecenes and zearalenone in cereals. Int. J. Mol. Sci. 9: 2062 — 2090.
Google Scholar
Yoshida M., Nakajima T. 2010. Deoxynivalenol and nivalenol accumulation in wheat infected with Fusarium graminearum during grain development. Phytopathology 100: 763 — 773.
Google Scholar
Zinedine A., Soriano J.M., Moltó J. C., Mañes J. 2007. Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: An oestrogenic mycotoxin. Food Chem. Toxicol. 45: 1 — 18.
Google Scholar
Authors
Tomasz Góralt.goral@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin - Państwowy Instytut Badawczy Poland
Authors
Halina WiśniewskaZakład Genomiki, Instytut Genetyki Roślin PAN Poland
Authors
Piotr OchodzkiInstytut Hodowli i Aklimatyzacji Roślin - Państwowy Instytut Badawczy Poland
Authors
Dorota Walentyn-GóralInstytut Hodowli i Aklimatyzacji Roślin - Państwowy Instytut Badawczy Poland
Authors
Iga GrzeszczakInstytut Hodowli i Aklimatyzacji Roślin - Państwowy Instytut Badawczy Poland
Authors
Jolanta BelterZakład Genomiki, Instytut Genetyki Roślin PAN Poland
Authors
Maciej MajkaZakład Genomiki, Instytut Genetyki Roślin PAN Poland
Authors
Jerzy BogackiDANKO Hodowla Roślin Poland
Authors
Tadeusz DrzazgaMałopolska Hodowla Roślin HBP Poland
Authors
Bogusława ŁugowskaDANKO Hodowla Roślin Poland
Authors
Przemysław MatysikHodowla Roślin Strzelce Grupa IHAR Sp. z o.o. Poland
Authors
Edward WitkowskiHodowla Roślin Smolice Sp. z o.o. Grupa IHAR
Authors
Krzysztof RubryckiPoznańska Hodowla Roślin Poland
Authors
Urszula Woźna-PawlakPoznańska Hodowla Roślin Poland
Statistics
Abstract views: 608PDF downloads: 100
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Tadeusz Adamski, Zygmunt Kaczmarek, Maria Surma, Anetta Kuczyńska, Karolina Krystkowiak, Bolesław Salmanowicz, Renata Trzeciak, Zofia Banaszak, Bogusława Ługowska, Małgorzata Majcher, Wiktor Obuchowski, Multivariate analysis of some quality traits of winter wheat grain , Bulletin of Plant Breeding and Acclimatization Institute: No. 260/261 (2011): Regular issue
- Mirosław Tyrka , Grzegorz Fic , Magdalena Szeliga , Marcin Jaromin , Paweł Krajewski , Paweł Milczarski , Tadeusz Drzazga , Przemysław Matysik , Róża Mazur , Teresa Sikora , Edward Witkowski , Justyna Buczkowicz , Dorota Tyrka , Genomic selection of winter wheat , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Elżbieta Małuszyńska, Tomasz Góral, Dorota Walentyn-Góral, Effect of pre-chilling on germination of infected by Fusarium spp. winter wheat kernels. , Bulletin of Plant Breeding and Acclimatization Institute: No. 284 (2018): Regular issue
- Tomasz Góral, Dorota Walentyn-Góral, Halina Wiśniewska, Fusarium head blight resistance of types I and II in wheat and triticale , Bulletin of Plant Breeding and Acclimatization Institute: No. 277 (2015): Regular issue
- Tadeusz Drzazga, Paweł Krajewski, Tadeusz Śmiałowski, Ewa Śmiałek, Drought resistance assessment of winter wheat cultivars , Bulletin of Plant Breeding and Acclimatization Institute: No. 260/261 (2011): Regular issue
- Tomasz Góral, Application of the detached-leaf technique to evaluate the pathogenicity of isolates of fungi Ascochyta fabae and Botrytis fabae to faba bean , Bulletin of Plant Breeding and Acclimatization Institute: No. 299 (2023): Regular issue
- Jakub Paderewski, Wiesław Mądry, Wiesław Pilarczyk, Tadeusz Drzazga, Retrospective study of yield response to environmental conditions in winter wheat cultivars using combined AMMI and cluster analysis to incomplete data: genetic progress for adaptability , Bulletin of Plant Breeding and Acclimatization Institute: No. 250 (2008): Regular issue
- Patrycja Siedlarz, Piotr Stefański, Przemysław Matysik, Zygmunt Nita, Krystyna Rybka, The effect of different LED illuminators on the germination index of wheat grains obtained in the greenhouse stage of the SSD breeding process , Bulletin of Plant Breeding and Acclimatization Institute: No. 282 (2017): Regular issue
- Tomasz Góral, Fusarium head blight of wheat. Part 1. Disease description and characteristics of pathogens , Bulletin of Plant Breeding and Acclimatization Institute: No. 300 (2023): Regular issue
- Tomasz Góral , Halina Wiśniewska , Paweł Czembor , Piotr Ochodzki , Magdalena Radecka-Janusik , Maciej Majka, Jarosław Przetakiewicz , Identification and application of phenotypic, metabolic and molecular markers in studies of types of resistance to Fusarium head blight in winter wheat accessions differing in resistance , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue