Characteristics of gluten proteins in breeding lines of wheat
Maciej Kała
m.kala@ihar.edu.plZakład Genomiki Funkcjonalnej, Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy w Radzikowie (Poland)
Mateusz Przyborowski
Zakład Genomiki Funkcjonalnej, Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy w Radzikowie (Poland)
Bogusława Ługowska
DANKO Hodowla Roślin Sp. z o.o. z/s w Choryni (Poland)
Sebastian Gasparis
Zakład Genomiki Funkcjonalnej, Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy w Radzikowie (Poland)
Anna Nadolska-Orczyk
Zakład Genomiki Funkcjonalnej, Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy w Radzikowie (Poland)
Abstract
Bread-making quality traits are very important parameters in wheat improvement. Gluten regulates viscoelastic properties of dough i.e. elasticity and extensibility. We examined high molecular weight glutenin subunits (HMW-GS) and ω-gliadins from breeding stocks and cultivars of winter and spring wheat, provided by three Polish breeding companies: HR Danko, HR Smolice and HR Strzelce. Proteins were separated on polyacrylamide gels using SDS-PAGE and A-PAGE methods. Among examined objects the most frequently represented (about 45%) subunit was Glu-B1(7+9). Subunits Glu-D1(5+10) and Glu-D1(2+12) were represented equally in all pools. The results provide the information about HMW-GS and ω-gliadins frequencies and diversity of the breeding lines in breeding companies.
Keywords:
A-PAGE, frequentation, diversity, glutenin, gliadins, SDS-PAGE, wheatReferences
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Authors
Maciej Kałam.kala@ihar.edu.pl
Zakład Genomiki Funkcjonalnej, Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy w Radzikowie Poland
Authors
Mateusz PrzyborowskiZakład Genomiki Funkcjonalnej, Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy w Radzikowie Poland
Authors
Bogusława ŁugowskaDANKO Hodowla Roślin Sp. z o.o. z/s w Choryni Poland
Authors
Sebastian GasparisZakład Genomiki Funkcjonalnej, Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy w Radzikowie Poland
Authors
Anna Nadolska-OrczykZakład Genomiki Funkcjonalnej, Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy w Radzikowie Poland
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