The genetic analysis of oilseed rape fatty acids composition on the basis of field experiments with doubled haploid lines
Elżbieta Adamska
office@igr.poznan.plInstytut Genetyki Roślin PAN w Poznaniu (Poland)
Teresa Cegielska-Taras
Instytut Hodowli i Aklimatyzacji Roślin w Poznaniu (Poland)
Laurencja Szała
Instytut Hodowli i Aklimatyzacji Roślin w Poznaniu (Poland)
Krystyna Czernik-Kołodziej
Instytut Hodowli i Aklimatyzacji Roślin w Poznaniu (Poland)
Abstract
The field trials were carried out at two sites: Plant Breeding and Acclimatization Institute experimental field in Poznań in the season 1998–1999 and the Experimental Station Institute of Plant Genetics Polish Academy of Science in Cerekwica in the season 1999–2000. Field experiments were conducted in randomized blocks, in three replications. Thirty-two lines of doubled haploid (DH) winter oilseed rape were obtained from F1 hybrid DH-O120 × DH- C-1041 using isolated microspore culture. Parental forms and F1 hybrids (in the first year) and F2 and F3 hybrids (in the second year) were studied. A content of the following fatty acids: palmitic acid (C16 : 0), stearic acid (C18 : 0), oleic acid (C18 : 1), linoleic acid (C18 : 2), linolenic acid (C18 : 3) was determined. Two-factor analysis of variance showed significant differences between the years of the study and genotypes. However the interaction genotypes × years was not significant with any of the acids. Based on DH lines and respective segregating generations, genetic parameters describing the effects of: additive genes (d), dominant genes (h) and non allelic interaction of homozygous (i) and, heterozygous (l) loci were estimated. The parameters were calculated for a content of fatty acids in each year (period) of the study and for two years in total. This work provides the example of how to determinate the means for genetic parameters in a two-year estimation period with the use of F1 and F2 hybrids in the first year and F2 and F3 hybrids in the second year.
Keywords:
DH lines, fatty acids, genetic parameters, oilseed rapeReferences
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Authors
Teresa Cegielska-TarasInstytut Hodowli i Aklimatyzacji Roślin w Poznaniu Poland
Authors
Laurencja SzałaInstytut Hodowli i Aklimatyzacji Roślin w Poznaniu Poland
Authors
Krystyna Czernik-KołodziejInstytut Hodowli i Aklimatyzacji Roślin w Poznaniu Poland
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Copyright (c) 2003 Elżbieta Adamska, Teresa Cegielska-Taras, Laurencja Szała, Krystyna Czernik-Kołodziej
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