Optimization of mutation detection methods in the NUD gene induced by CRISPR / CAS9 technology in barley (Hordeum vulgare L.)
Mateusz Przyborowski
m.przyborowski@ihar.edu.plZakład Genomiki Funkcjonalnej,2 Zakład inżynierii Genetycznej Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików (Poland)
https://orcid.org/0000-0001-7456-3808
Sebastian Gasparis
Zakład Genomiki Funkcjonalnej,2 Zakład inżynierii Genetycznej Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików (Poland)
https://orcid.org/0000-0003-0521-086X
Wacław Orczyk
Zakład Genomiki Funkcjonalnej,2 Zakład inżynierii Genetycznej Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików (Poland)
https://orcid.org/0000-0003-4735-0000
Anna Nadolska-Orczyk
Zakład Genomiki Funkcjonalnej,2 Zakład inżynierii Genetycznej Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików (Poland)
https://orcid.org/0000-0001-6127-3860
Abstract
The aim of the study was to select the optimal method for mutation detection in the Nud gene induced by using the CRISPR / Cas9 technology in barley. Four commonly used genotyping techniques were tested: restriction fragment length polymorphism (RFLP), enzymatic digestion using endonuclease I from T7 bacteriophage, high resolution melting curve of polymerase chain reaction product (HRM-PCR) and molecular probes. Findings in the current study suggest that the most favorable way for detecting mutations induced by the CRISPR / Cas9 technique is a method based on molecular probes. It enables to receive clear and repeatable results but at the same time requires the use of advanced equipment.
Keywords:
CRISPR / Cas9, genome editing, Nud gene, barley, molecular probesAuthors
Mateusz Przyborowskim.przyborowski@ihar.edu.pl
Zakład Genomiki Funkcjonalnej,2 Zakład inżynierii Genetycznej Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików Poland
https://orcid.org/0000-0001-7456-3808
Authors
Sebastian GasparisZakład Genomiki Funkcjonalnej,2 Zakład inżynierii Genetycznej Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików Poland
https://orcid.org/0000-0003-0521-086X
Authors
Wacław OrczykZakład Genomiki Funkcjonalnej,2 Zakład inżynierii Genetycznej Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików Poland
https://orcid.org/0000-0003-4735-0000
Authors
Anna Nadolska-OrczykZakład Genomiki Funkcjonalnej,2 Zakład inżynierii Genetycznej Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików Poland
https://orcid.org/0000-0001-6127-3860
Statistics
Abstract views: 237PDF downloads: 170
License
![Creative Commons License](http://i.creativecommons.org/l/by-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Anna Nadolska-Orczyk, Karolina Barchacka, Sebastian Gasparis, Hanna Ogonowska, Maciej Kała, Identification of genetic variation of wheat correlating with grain yield and development of root system , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Wacław Orczyk, Marta Dmochowska-Boguta , Yuliya Kloc , Tolerance for abiotic stresses — genotyping of wheat based on candidate gene strategy , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Przemysław Werecki, Marta Dmochowska-Boguta, Anna Nadolska-Orczyk, Wacław Orczyk, Markers of wheat resistance types on Puccinia triticina , Bulletin of Plant Breeding and Acclimatization Institute: No. 287 (2019): Special issue
- Maciej Kała, Mateusz Przyborowski, Bogusława Ługowska, Sebastian Gasparis, Anna Nadolska-Orczyk, Characteristics of gluten proteins in breeding lines of wheat , Bulletin of Plant Breeding and Acclimatization Institute: No. 282 (2017): Regular issue
- Joanna Bocian, Anna Nadolska-Orczyk, Contribution of cytokinin biosynthesis genes in determining wheat grain yield , Bulletin of Plant Breeding and Acclimatization Institute: No. 300 (2023): Regular issue
- Sebastian Gasparis, Anna Nadolska-Orczyk, Genetic background of wheat and triticale grain hardness , Bulletin of Plant Breeding and Acclimatization Institute: No. 267 (2013): Regular issue
- Maciej Kała, Mateusz Przyborowski, Sebastian Gasparis, Wacław Orczyk, Anna Nadolska-Orczyk, Classification of glutenin subunits using machine learning methods , Bulletin of Plant Breeding and Acclimatization Institute: No. 283 (2018): Special issue
- Mateusz Przyborowski, Sebastian Gasparis, Maciej Kała, Wacław Orczyk, Anna Nadolska-Orczyk, Frequency of puroindoline alleles in landraces of common wheat (Triticum aestivum L.) from National Centre for Plant Genetic Resources: Polish Genebank , Bulletin of Plant Breeding and Acclimatization Institute: No. 283 (2018): Special issue
Similar Articles
- Ewelina Żmijewska , Study on safety of GMO , Bulletin of Plant Breeding and Acclimatization Institute: No. 287 (2019): Special issue
- Teresa Oracka, Zbigniew Rybka , Bogusław Łapiński , The influence of homoeologous D(A) and D(B) substitutions on plant dry matter, nitrogen and phosphorus accumulation and utilization efficiency in hexaploid triticale young plants grown in hydroponics , Bulletin of Plant Breeding and Acclimatization Institute: No. 288 (2020): Regular issue
- Dariusz Grzebelus, Gabriela Machaj, Alicja Macko-Podgórni, Kornelia Kwolek, Rafał Barański, Wojciech Wesołowski, Marcelina Wajdzik, Anna Szlachtowska, Beata Domnicz, Przemysław Gierski, Marek Szklarczyk, Development of methods for global analysis of polymorphisms in the genome of sugar beet , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Marta Puchta, miRNAs involved in the seed ageing and germination process , Bulletin of Plant Breeding and Acclimatization Institute: No. 290 (2020): Special issue
- Dorota Michałowska, Agnieszka Przewodowska, Joanna Piskorz, Paulina Buryło, Polish potato cultivars at the Gene Bank in vitro , Bulletin of Plant Breeding and Acclimatization Institute: No. 283 (2018): Special issue
- Marcin Beza, The Kostrzyca Forest Gene Bank activities in the area of forest genetic resources conservation , Bulletin of Plant Breeding and Acclimatization Institute: No. 283 (2018): Special issue
- Włodzimierz Majtkowski, Evolution of the priorities of collection and preservation of gene grass resources by the Botanical Garden of National Centre for Plant Genetic Resources in Bydgoszcz in the years 1971–2017 , Bulletin of Plant Breeding and Acclimatization Institute: No. 283 (2018): Special issue
- Małgorzata Łabańska, Włodzimierz Przewodowski , Biosensors – novel analytical tools for the plant pathogen detection , Bulletin of Plant Breeding and Acclimatization Institute: No. 290 (2020): Special issue
- Irena Kolasińska, Results of the breeding program aimed at improvement of male fertility of hybrid rye varieties. , Bulletin of Plant Breeding and Acclimatization Institute: No. 284 (2018): Regular issue
- Maciej Kała, Mateusz Przyborowski, Bogusława Ługowska, Sebastian Gasparis, Anna Nadolska-Orczyk, Characteristics of gluten proteins in breeding lines of wheat , Bulletin of Plant Breeding and Acclimatization Institute: No. 282 (2017): Regular issue
<< < 10 11 12 13 14 15 16 17 18 19 20 > >>
You may also start an advanced similarity search for this article.