Polymorphism of gliadins and HMW glutenins and variability in quality traits in hybrid genotypes of spelt and common wheat
Jacek Waga
j.waga@ihar.edu.plZakład Roślin Zbożowych, Instytut Hodowli i Aklimatyzacji Roślin, Kraków (Poland)
Maria Stachowicz
Zakład Roślin Zbożowych, Instytut Hodowli i Aklimatyzacji Roślin, Kraków (Poland)
Katarzyna Kraska
Zakład Roślin Zbożowych, Instytut Hodowli i Aklimatyzacji Roślin, Kraków (Poland)
Abstract
Spelt wheat cultivar Oberkummler Rotkorn and common wheat breeding line LAD 480 differ in the composition of storage proteins and in some important quality traits. Moreover, cv. Oberkummler Rotkorn contains two specific gliadin blocks coded by chromosomes 1B and 6B (Gli B1-6 and Gli B2-3). Such blocks have not so far been observed among wheat genotypes cultivated and examined in Poland. The aim of the study was to estimate the genetic relationships between spelt specific gliadin protein blocks and variability in sedimentation value, total protein content and gluten content as well as rheological properties evaluated by farinograph and extensograph analyses. Differentiation of average values for quality traits in closely related F5 hybrid genotypes was estimated by Student t test. Significant differences in sedimentation values between the genotypes varying in gliadins coded by the chromosome 6B, but not 1B, were found. In hybrid lines differing in three gliadin coding loci (Gli B1, Gli D1 and Gli A2) significant differences in sedimentation value, protein content and rheological parameters were observed. As the HMW glutenin subunits in all the genotypes analyzed were uniform, a conclusion can be drawn that the examined gliadin protein blocks were the main factors responsible for the observed differentiation in technological parameters.
Keywords:
spelt, gliadins, glutenins, wheat qualityReferences
Abdel-Aal E. S. M., Salama D. A., Hucl P., Sosulski F. W., Cao W. 1996 a. Electrophoretic characterization of spring spelt wheat gliadins. J. Agric. Food Chem. 44: 2117 — 2123.
Google Scholar
Axford D. W. E., McDermott E. E., Redman D. G. 1979. Note on the sodium dodecyl sulfate test of breadmaking quality: comparison with Pelshenke and Zeleny tests. Cereal Chem. 56: 582 — 584.
Google Scholar
Bietz J. A. 1997. Recent advances in the isolation and characterization of cereal proteins. Cereal Foods World 24:199 — 202.
Google Scholar
Bonafaccia G., Galli V., Francisci R., Mair V., Skranbanja V., Kreft I. 2000. Characteristics of spelt wheat products and nutritional value of spelt wheat-based bread. Food Chem. 68: 437 — 441.
Google Scholar
Branlard G.P., Dardevet M., Saccomano R., Lagoutte F., Gourdon J. 2001. Genetic diversity of wheat storage proteins and bread wheat quality. Euphytica 119: 59 — 67.
Google Scholar
Branlard G.P., Metakovsky E.V. Some Gli alleles related to common wheat dough quality. In: Gliadin and glutenin. The unique balance of wheat quality. Wrigley C., Bekes F., Bushuk W. eds. AACC International, St. Paul, Minnesota, USA. 2007: 115 — 139.
Google Scholar
Bushuk W., Zillman R. R. 1978. Wheat cultivar identification by gliadin electrophoregrams. I. Apparatus, method and nomenclature. Can. J. Plant Sci. 58:505 — 515.
Google Scholar
Federmann G. R., Goecke E. U., Steiner A. M. 1992. Research note: Detection of adulteration of flour of spelt (Triticum spelta L.) with flour of wheat (Triticum aestivum L. emend. Fiori et Paol.) by electrophoresis. Plant Var. Seeds. 5:123 — 125.
Google Scholar
ICC. 1992. Standard methods of the International Association for Cereal Science and Technology. Standard No. 114/1. Method for using the Brabender Extesograph. The Association: Detmold, Germany.
Google Scholar
ISO. 1988. International Organization for Standardization. Determination of water absorption and rheological properties using a farinograph. 5530-1. ISO: Geneva.
Google Scholar
Leammli U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680 — 685.
Google Scholar
MacKey J. 1966. Species relationships in Triticum. Proc. Int. Wheat Genet. Symp., 2nd. (Hereditas Suppl.). 2: 237 — 276.
Google Scholar
MacRitchie F. 1992. Physicochemical properties of wheat proteins in relation to functionality. Adv. Food Nut. Res. 36: 1 — 85.
Google Scholar
Payne P. I., Lawrence G. J. 1983. Catalogue of alleles for the complex gene loci GluA1, GluB1, GluD1, which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res. Commun., 11/1: 29 — 35.
Google Scholar
Piergiovanni A. R., Blanco A. 1999. Variation of HMW glutenin and γ-gliadin subunits in selected accessions of Triticum dicoccon (Shrank) and T. spelta (L.). Cereal Res. Comm. 27:205 — 211.
Google Scholar
Ranhotra G. S., Gelroth J. A., Glaser B. K., Lorenz K. J. 1995. Baking and nutritional qualities of a spelt wheat sample. Lebensm. Wiss. Technol. 28:118 — 122.
Google Scholar
Schober T. J., Bean S. R., Kuhn M. 2006. Gluten proteins from spelt (Triticum aestivum ssp. spelta) cultivars: a rheological and size-exclusion high-performance liquid chromatography study. J. Cereal Sci. 44: 161 — 173.
Google Scholar
Shewry P. R., Tatham A. S. 1997. Disulphide bonds in wheat gluten proteins. J. Cereal Sci. 25:207 — 227.
Google Scholar
Shewry P. R., Halford N. G., Belton P. S., Tatham A. S. 2003. Gluten, the elastomeric protein of wheat seed. In: Elastomeric Proteins. Shewry P. R., Tatham A. S., Bailey A. J. Eds. Cambridge University Press: 279 — 301.
Google Scholar
Waga J. 1997. Polimorfizm białek gliadynowych i gluteninowych a jakość pszenicy ozimej (Triticum aestivum L.), Biul. IHAR 204: 205 — 218.
Google Scholar
Waga J. 2003. Zmienność niektórych frakcji -gliadyn a zawartość białka ogółem w potomstwie orkiszu i odmiany Elena. Biul. IHAR 228: 61 — 69.
Google Scholar
Waga J. 2005. Dziedziczenie wybranych frakcji białek gliadynowych u mieszańców orkiszu z pszenicą zwyczajną. Biul. IHAR 236: 25 — 40.
Google Scholar
Waga J. 2007. Katalog bloków białek gliadynowych polskich odmian i rodów pszenicy zwyczajnej. Biul. IHAR 243:3 — 23.
Google Scholar
Wrigley C. W., Bekes F., Bushuk W. 2006. Gluten: a balance of gliadin and glutenin. In: Gliadin and glutenin — the unique balance in wheat quality. Eds. C. Wrigley, F. Bekes and W. Bushuk: 3 — 35.
Google Scholar
Zeleny L., Greenway W. T., Gurney G. M., Fifield C. C., Lebsock K. L. 1960. Sedimentation value as the index of dough mixing characteristics in early-generation wheat selections. Cereal Chem. 37: 673.
Google Scholar
Authors
Jacek Wagaj.waga@ihar.edu.pl
Zakład Roślin Zbożowych, Instytut Hodowli i Aklimatyzacji Roślin, Kraków Poland
Authors
Maria StachowiczZakład Roślin Zbożowych, Instytut Hodowli i Aklimatyzacji Roślin, Kraków Poland
Authors
Katarzyna KraskaZakład Roślin Zbożowych, Instytut Hodowli i Aklimatyzacji Roślin, Kraków Poland
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