Estimation of genetic variability and of β-ODAP neurotoxin content in chosen species of the genus Lathyrus
Katarzyna Pankiewicz
office@igr.poznan.plInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Wojciech Rybiński
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Abstract
The aim of the study was to estimate of genetic variability among species chosen from the Lathyrus collection with the use of polymorphic random amplified DNA fragments and to analyze β-N-oxalyl-L-α-β-diaminopropionic acid content (β-ODAP) of seeds. Plants of four species: L. sativus, L. cicera, L. tingitanus and L. gorgoni were evaluated. RAPD-PCR reaction for twenty-eight 10- nucleotide primers (with random sequence) showed 97% polymorphism. Coefficients of genetic similarity, calculated according to Nei’s method, ranged from 0.21 for a pair L. tingitanus - L. cicera to 0.53 for L. sativus - L. cicera. The highest intraspecies similarity was observed for accessions of L. sativus (0.84), and the lowest one for accessions of L. tingitanus (0.38). Content of β-ODAP neurotoxin was analyzed with the use of specific reaction with o-phthalaldehyde (OPT) and absorbance measured at by 425 nm. The highest content of neurotoxin in dry matter of seeds (1g/kg d.m.) was found in L. tingitanus and L. gorgoni. Comparatively, the content of neurotoxin recorded for seeds of one of the accessions derived from L. sativus was 20- fold lower.
Keywords:
Lathyrus, β-ODAP, RAPD, genetic similarityReferences
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Authors
Katarzyna Pankiewiczoffice@igr.poznan.pl
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Wojciech RybińskiInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
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