Use of molecular and phenotypic markers to identify wheat eyespot resistance genes caused by Oculimacula yallundae and O. acuformis
Maciej Majka
Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0002-5039-9465
Magdalena Gawłowska
Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0002-8975-0208
Adriana Twardawska
Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0003-1139-8133
Marek Korbas
Instytut Ochrony Roślin — Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań (Poland)
https://orcid.org/0000-0002-5536-1853
Jakub Danielewicz
Instytut Ochrony Roślin — Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań (Poland)
https://orcid.org/0000-0003-0252-8573
Tomasz Góral
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, 05-870 Błonie (Poland)
https://orcid.org/0000-0001-9130-6109
Bogusława Ługowska
DANKO Hodowla Roślin, Choryń 27, 64-000 Kościan (Poland)
Jolanta Belter
Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0003-1934-448X
Edward Witkowski
Hodowla Roślin Smolice, Grupa IHAR, Smolice 134, 63-740 Kobylin (Poland)
Tadeusz Drzazga
Małopolska Hodowla Roślin, ul. Sportowa 21, 55-040 Kobierzyce (Poland)
Przemysław Matysik
Hodowla Roślin Strzelce, Grupa IHAR, Główna 20, 99-307 Strzelce (Poland)
Urszula Woźna-Pawlak
Poznańska Hodowla Roślin, Antoniny 2, 64-100 Leszno (Poland)
https://orcid.org/0000-0002-1454-6181
Halina Wiśniewska
hwis@igr.poznan.plInstytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań (Poland)
https://orcid.org/0000-0003-2782-685X
Abstract
Eyespot is one of the most important diseases of wheat (Triticum aestivum L.), caused by two pathogenic fungi Oculimacula yallundae and Oculimacula acuformis. There are several sources of resistance to this pathogen, but so far only two genes Pch1 and Pch2 have been transferred to wheat and confer the resistance. The selection of the most favorable genetic markers for determining the presence of eyespot resistance genes may improve the efficiency and accuracy in the selection of wheat genotypes resistant for this disease. The aim of the work was to determine the effectiveness of molecular markers and protein marker for Pch1 and Pch2 genes and to select genotypes of winter wheat with increased resistance in relation to plant infection in inoculation tests carried out at the seedling and adult plant stage. Plant material consist of 159 breeding lines of winter wheat and five control varieties: Artist, Kilimanjaro, Kometa, Patras and Rendezvous. To identify resistant genes, five molecular markers were used. Three for Pch1 gene (EpD1b, XustSSR2001-7DL, Xorw1) and two for Pch2 gene (Xcfa2040, Xwmc525). Considering the molecular analysis of genes, results of seedlings inoculation and the results of plant stem infestation, it was found that there were no infection symptoms of stalks in the winter wheat lines and/or varieties, in which both Pch1 and Pch2 genes were identified. In these lines/varieties, the lowest infection of seedlings was also observed. The highest percentage of infected sheaths was identified in genotypes where Pch1 and Pch2 genes were not found. The highest seedling infection was also observed for these genotypes. It was shown that the presence of Pch1 and Pch2 genes or their absence did not significantly affect the grain yield and the thousands kernels weight (TKW). Genotypes with the Pch1 and Pch2 genes showed slightly higher values for both technological parameters.
Supporting Agencies
Keywords:
resistance genes, eyespot, Pch1, Pch2, Triticum aestivumReferences
Burt C., Hollins T. W., Nicholas P. 2014. Identification of a QTL conferring seedling and adult plant resistance to eyespot on chromosome 5A of Cappelle Desprez. Theor. Appl. Genet. 122: 119 — 128.
Google Scholar
Chapman N., Burt C., Dong H., Nicholson P. 2008. The development of PCR-based markers for the selection of eyespot resistance genes Pch1 and Pch2. Theor Appl Genet 117: 425 — 433.
Google Scholar
Deighton F. C. 1973. Studies on Cercospora and allied genera. IV. Cercosporella Sacc., Pseudocercosporella gen. nov. and Pseudocercosporidium gen. nov. Mycological Papers 133:1 — 62.
Google Scholar
Dumalasova V., Palicova J., Hanzalova A., Bizova I., Laisova-Svobodova L. 2015. Eyespot Resistance Gene Pch1 and Methods of Study of its Effectiveness in Wheat Cultivars. Czech J Genet Plant Breed, 51 (4): 166 —173.
Google Scholar
EPPO no. PP 1/28 — Standards — PP1 Efficiency of plant protection products — Summary list of approved Standards (2018 – 09).
Google Scholar
Fitt B. D. L., Goulds A., Polley R. W. 1988. Eyespot (Pseudocercosporella herpotrichoides) epidemiology in relation to prediction of disease severity and yield loss in winter wheat — a Review. Plant Pathology 37: 311 — 328.
Google Scholar
Fron M. G. 1912. Contribution a l'etude de la maladie due 'piet noir des cereales' ou 'maladie du pietin'. Annales de la Science Agrnomique, France IV: 1.
Google Scholar
Groenewald J. Z., Marais A. S., Marais G. F. 2003. Amplified fragment length polymorphism-derived microsatellite sequence linked to the Pch1 and Ep-D1 loci in common wheat. Plant Breeding 122(1): 83 — 85.
Google Scholar
Jahier J., Doussinault G., Dosba F., Bourgeois F. 1978. Monosomic analysis of resistance to eyespot in the variety „Roazon”. In: Proceedings of the Fifth International Wheat Genetics Symposium, Luty 23–28, New Delhi, Indie: 437 — 440.
Google Scholar
Johnson R. 1992. Past, present and future opportunities in breeding for disease resistance, with examples from wheat. Euphytica 63: 3 —22.
Google Scholar
Koen E., Labuschagne, M. T., Viljoen, C. D. 2002. The influence of eyespot resistance genes on baking quality and yield in wheat. Journal of the Science of Food and Agriculture 82: 1537 — 1540.
Google Scholar
Korbas M., Parandowski A., Węgorek P., Jajor E., Horoszkiewicz-Janka J., Zamojska J., Danielewicz J., Czyczewski M., Dworzańska D. 2017. Vademecum środków ochrony roślin, Wyd. Agronom, Poznań.
Google Scholar
Kwiatek M., Pankiewicz K., Wiśniewska H., Korbas M., Danielewicz J. 2012. Identification of Pch1 eyespot resistance gene in the collection of wheat lines (Triticum Aestivum L.). Journal of Plant Protection Research 52 (2): 254 — 258.
Google Scholar
Kwiatek M., Wiśniewska H., Kaczmarek Z., Korbas M., Gawłowska M., Majka M., Pankiewicz K., Danielewicz J., Belter J. 2015. Using markers and field evaluation to identify the source of eyespot resistance gene Pch1 in the collection of wheat breeding lines. Cereal Research Communications 43: 638 — 648.
Google Scholar
Kwiatek M., Wiśniewska H., Korbas M., Gawłowska M., Belter J., Majka M., Danielewicz J. 2016. How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield? Journal of Plant Protection Research 56: 319 — 322.
Google Scholar
Leonard J. M., Watson C. J., Carter A. H., Hansen J. L., Zemetra R. S., Santra D. K., Campbell K. G., Riera-Lizarazu O. 2008. Identification of a candidate gene for the wheat endopeptidase EpD1 locus and two other STS markers linked to the eyespot resistance gene Pch1. Theor. Appl. Genet. 116: 261 — 270.
Google Scholar
Maia N. 1967. Obtention de blés tendres résistants au piétin-verse (Cercosporella herpotrichoides) par crôisements interspécifiques blés x Aegilops. Comptes Rendus de l'Académie d'Agriculture de France 53: 149 — 155.
Google Scholar
Majka M., Kwiatek M., Korbas M., Danielewicz J., Gawłowska M., Góral T., Wiśniewska H. 2018. Eyespot resistance of winter wheat breeding lines evaluated with the marker-assisted selection and inoculation tests at the seedling and adult plant stage, Journal of Plant Protection Research 58:387 — 394.
Google Scholar
McMillin D. E., Allan R. E., Roberts D. E. 1986. Association of an isozyme locus and strawbreaker foot rot resistance derived from Aegilops ventricosa in wheat. Theor. Appl. Genet. 72: 743 — 747.
Google Scholar
Mena M., Doussinault G., Lopez-Braña I., Aguaded S., García-Olmedo F., Delibes A. 1992. Eyespot resistance gene Pch-1 in H-93 wheat lines. Evidence of linkage to markers of chromosome group 7 and resolution from the endopeptidase locus Ep-D1b. Theor. Appl. Genet. 83: 1044 — 1047.
Google Scholar
Meyer N., Lind V., Heindorf M., Korzun V., Friedt W., Ordon F. 2011. Diagnostic value of molecular markers linked to the eyespot resistance gene Pch1 in wheat. Euphytica 177: 267 — 275.
Google Scholar
Muranty H., Jahier J., Tanguy A. M., Worland A. J., Law C. 2002. Inheritance of resistance of wheat to eyespot at the adult stage. Plant Breeding 121: 536 — 538.
Google Scholar
Murray T. D. 2010. Eyespot (strawbreaker foot rot). In: Compendium of Wheat Diseases and Pests (Bockus W. W. Bowden R. L., Hunger R. M., Murray T. D., Smiley R. W., Morrill W. ed.). 3rd ed. APS Press, Minneapolis 171: 32 — 34.
Google Scholar
Pasquariello M., Ham J., Burt C., Jahier J., Paillard S., Uauy C., Nicholson P. 2017. The eyespot resistance genes Pch1 and Pch2 of wheat are not homoeoloci. Theor. Appl. Genet. 130: 91 —107.
Google Scholar
Santra D. K., Watt C., Little L., Kidwell K. K., Campbell K. G. 2006. Comparison of a modified assay method for the endopeptidase marker Ep-D1b with the Sequence Tag Site marker XustSSR2001-7DL. Plant Breeding 125: 13 — 18.
Google Scholar
Wiśniewska H., Kwiatek M., Majka M., Gawłowska M., Belter J. 2016. Selekcja pszenicy odpornej na łamliwość podstawy źdźbła z wykorzystaniem markerów izoenzymatycznych i molekularnych. (W:) Metody biotechnologiczne w hodowli pszenicy — wybrane zagadnienia (red.) Surma M., Kuczyńska A., Adamski T., Wydawnictwo ProDRUK Poznań, ISBN 978-83-64246-33-3: 27 — 35.
Google Scholar
Wiśniewska H., Majka M., Kwiatek M., Gawłowska M., Surma M., Adamski T., Kaczmarek Z., Drzazga T., Ługowska B., Korbas M., Belter J. 2019. Production of wheat doubled haploids resistant to eyespot supported by marker-assisted selection, Electronic Journal of Biotechnology 37:11 — 17.
Google Scholar
Worland A. J., Law C. N., Hollins T. W., Koebner R. M. D., Giura A. 1988. Location of a gene for resistance to eyespot (Pseudocercosporella herpotrichoides) on chromosome 7D of bread wheat. Plant Breeding 101: 43 — 51.
Google Scholar
Authors
Maciej MajkaInstytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0002-5039-9465
Authors
Magdalena GawłowskaInstytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0002-8975-0208
Authors
Adriana TwardawskaInstytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0003-1139-8133
Authors
Marek KorbasInstytut Ochrony Roślin — Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań Poland
https://orcid.org/0000-0002-5536-1853
Authors
Jakub DanielewiczInstytut Ochrony Roślin — Państwowy Instytut Badawczy, Władysława Węgorka 20, 60-318 Poznań Poland
https://orcid.org/0000-0003-0252-8573
Authors
Tomasz GóralInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Radzików, 05-870 Błonie Poland
https://orcid.org/0000-0001-9130-6109
Authors
Bogusława ŁugowskaDANKO Hodowla Roślin, Choryń 27, 64-000 Kościan Poland
Authors
Jolanta BelterInstytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0003-1934-448X
Authors
Edward WitkowskiHodowla Roślin Smolice, Grupa IHAR, Smolice 134, 63-740 Kobylin Poland
Authors
Tadeusz DrzazgaMałopolska Hodowla Roślin, ul. Sportowa 21, 55-040 Kobierzyce Poland
Authors
Przemysław MatysikHodowla Roślin Strzelce, Grupa IHAR, Główna 20, 99-307 Strzelce Poland
Authors
Urszula Woźna-PawlakPoznańska Hodowla Roślin, Antoniny 2, 64-100 Leszno Poland
https://orcid.org/0000-0002-1454-6181
Authors
Halina Wiśniewskahwis@igr.poznan.pl
Instytut Genetyki Roślin Polskiej Akademii Nauk, Strzeszyńska 34, 60-479 Poznań Poland
https://orcid.org/0000-0003-2782-685X
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