Quantitative changes in DNA methylation induced by monochromatic light in barley regenerants obtained by androgenesis
Patrycja Siedlarz
Department of Plant Biochemistry and Physiology, Plannt Breeding and Acclimatization Institute - National Research Institute, 05-870 Radzików, Poland (Poland)
https://orcid.org/0000-0001-5986-7790
Sławomir Bany
Department of Plant Biochemistry and Physiology, Plannt Breeding and Acclimatization Institute - National Research Institute, 05-870 Radzików, Poland (Poland)
Krystyna Rybka
k.rybka@ihar.edu.plDepartment of Plant Biochemistry and Physiology, Plannt Breeding and Acclimatization Institute - National Research Institute, 05-870 Radzików, Poland (Poland)
https://orcid.org/0000-0002-4707-8492
Abstract
Changes in DNA methylation are one of the best known mechanisms of epigenetic regulation of gene expression, which in the process of induced androgenesis is associated with reprogramming of haploid microspores development towards the formation of embryos, as a result of exposure of anthers in ears and then anthers culture in vitro to stress factors. The aim of the study was to test the hypothesis of whether the use of monochromatic light during induced androgenesis might be associated with epigenetic phenomena. The experiments were carried out on DH plants of spring barley (Hordeum vulgare L.) obtained by androgenesis modified by monochromatic light: blue, green and red. A quantitative evaluation of the effect of light on the degree of DNA methylation was performed using RP-HPLC for the comparison of regenerants obtained under standard, control conditions (darkness) with those obtained with light usage. The differences in the amount of methylated cytidine in comparison to the control were: 0.40%, 0.16% and -0.55%, for blue, red and green light, respectively. The level of global genomic DNA methylation from control plants was in the range 21.32-21.52%. Methylation changes in response to monochromatic light used during callus formation in anthers culture, determined by RP-HPLC, are significant although small.
Supporting Agencies
Keywords:
RP-HPLC, Hordeum vulgare L., LED, cytidineReferences
Bednarek P. T., Orłowska R. 2020. Plant tissue culture environment as a switch-key of (epi)genetic changes. Plant Cell Tiss. Organ Cult. 140: 245 – 257.
Google Scholar
Finnegan E. J., Genger R. K., Peacock W. J., Dennis E. S. 1998. DNA methylation in plants. Ann. Rev. Plant Physiol. Plant Mol. Biol. 49(1): 223 – 247.
Google Scholar
Fiuk A., Bednarek P. T., Rybczyński J. J. 2010. Flow Cytometry, HPLC-RP, and metAFLP Analyses to Assess Genetic Variability in Somatic Embryo-Derived Plantlets of Gentiana pannonica Scop. Plant Mol. Biol. Rep. 28: 413 – 420.
Google Scholar
Guz J., Foksiński M., Oliński R. 2010. Mechanizm metylacji i demetylacji DNA – znaczenie w kontroli ekspresji genów [Eng.: Mechanism of DNA methylation and demethylation – its role in control of genes expression]. Post. Bioch. 56(1): 7 – 15.
Google Scholar
Johnston J. W., Harding K., Bremner D. H., Souch G., Green J., Lynch P. T., Grout B., Benson E. E. 2005. HPLC analysis of plant DNA methylation: a study of critical methodological factors. Plant Physiol. Biochem. 43(9): 844 – 853.
Google Scholar
Karan R., DeLeon T., Biradar H., Subudhi P. K. 2012. Salt stress induced variation in DNA methylation pattern and its influence on gene expression in contrasting rice genotypes. PloS One 7(6): e40203.
Google Scholar
Law J. A., Jacobsen S. E. 2010. Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat. Rev. Gen. 11(3): 204 – 220.
Google Scholar
Li X., Yu X., Wang N., Feng Q., Dong Z., Liu L., Liu B. 2007. Genetic and epigenetic instabilities induced by tissue culture in wild barley (Hordeum brevisubulatum Trin.). Plant Cell Tiss. Organ Cult. 90(2): 153 – 168.
Google Scholar
Machczyńska J., Orłowska R., Zimny J., Bednarek P. T. 2014. Extended metAFLP approach in studies of the tissue culture induced variation (TCIV) in case of triticale Mol. Breed. 34(3): 845 – 854.
Google Scholar
Niedziela A. 2018. The influence of Al3+ on DNA methylation and sequence changes in the triticale (× Triticosecale Wittmack) genome. J. Appl. Gen. 59(4): 405 – 417.
Google Scholar
Orłowska R., Machczyńska J., Oleszczuk S., Zimny J., Bednarek P. T. 2016. DNA methylation changes and TE activity induced in tissue cultures of barley (Hordeum vulgare L.). J. Biol. Res.-Thessalonike 23(1): 19.
Google Scholar
Pandey N., Pandey-Rai S. 2015. Deciphering UV-B-induced variation in DNA methylation pattern and its influence on regulation of DBR2 expression in Artemisia annua L. Planta 242(4): 869 – 879.
Google Scholar
Parrilla-Doblas J. T., Roldan-Arjona T., Ariza R. R., Cordoba-Canero D. 2019. Active DNA Demethylation in Plants. Int. J. Mol. Sci. 20(19): 4683.
Google Scholar
Peredo E. L., Revilla M. Á., Arroyo-García R. 2006. Assessment of genetic and epigenetic variation in hop plants regenerated from sequential subcultures of organogenic calli. J. Plant Physiol. 163(10): 1071 – 1079.
Google Scholar
Siedlarz et al. 2020 (w przygotowaniu).
Google Scholar
Zhang H., Zhu J. K. 2012. Active DNA demethylation in plants and animals. Cold Spring Harb. Symp. Quant. Biol. 77: 161 – 173.
Google Scholar
Authors
Patrycja SiedlarzDepartment of Plant Biochemistry and Physiology, Plannt Breeding and Acclimatization Institute - National Research Institute, 05-870 Radzików, Poland Poland
https://orcid.org/0000-0001-5986-7790
Authors
Sławomir BanyDepartment of Plant Biochemistry and Physiology, Plannt Breeding and Acclimatization Institute - National Research Institute, 05-870 Radzików, Poland Poland
Authors
Krystyna Rybkak.rybka@ihar.edu.pl
Department of Plant Biochemistry and Physiology, Plannt Breeding and Acclimatization Institute - National Research Institute, 05-870 Radzików, Poland Poland
https://orcid.org/0000-0002-4707-8492
Statistics
Abstract views: 437PDF downloads: 198 PDF downloads: 93
License
Copyright (c) 2020 Patrycja Siedlarz, Sławomir Bany, Krystyna Rybka
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Piotr Stefański, Patrycja Siedlarz, Przemysław Matysik, Zygmunt Nita, Krystyna Rybka, The usefulness of light sources based on diodes characterized by a continuous spectrum of white light enriched with a blue band in cereal breeding , Bulletin of Plant Breeding and Acclimatization Institute: No. 284 (2018): Regular issue
- Patrycja Siedlarz, Piotr Stefański, Przemysław Matysik, Zygmunt Nita, Krystyna Rybka, The effect of different LED illuminators on the germination index of wheat grains obtained in the greenhouse stage of the SSD breeding process , Bulletin of Plant Breeding and Acclimatization Institute: No. 282 (2017): Regular issue
- Renata Orłowska, Piotr T. Bednarek, Sławomir Bany, Molecular characterization of the impact of transposable elements on genetic variation in cereals tissue cultures , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Piotr Stefański, Krystyna Rybka, Przemysław Matysik, Phenotyping of winter triticale canopy density in field conditions using an RGB camera , Bulletin of Plant Breeding and Acclimatization Institute: No. 301 (2024): Regular issue
- Piotr Bednarek, Agnieszka Niedziela, Marzena Wasiak, Sławomir Bany , Identification of pollen fertility restoration markers in rye (Secale cereale L.) with CMS-Pampa , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Krystyna Rybka, The latest news in the field of biotechnology and grain breeding: CBB7- seventh Cereal Biotechnology and Breeding conference in Wernigerode, Germany , Bulletin of Plant Breeding and Acclimatization Institute: No. 300 (2023): Regular issue
- Krystyna Rybka, Plant phenotyping. The EPPN 2020 Conference in Tartu/ Estonia , Bulletin of Plant Breeding and Acclimatization Institute: No. 282 (2017): Regular issue
- Krystyna Rybka, Zygmunt Nita, Modern phenotypes of cereals for growing in areas endangered with drought , Bulletin of Plant Breeding and Acclimatization Institute: No. 273 (2014): Regular issue
Similar Articles
- Jerzy H. Czembor, Aleksandra Pietrusińska, Urszula Piechota, Hordeum bulbosum — as a source of effective resistance to barley leaf rust , Bulletin of Plant Breeding and Acclimatization Institute: No. 281 (2017): Regular issue
- Piotr Stefański, Patrycja Siedlarz, Przemysław Matysik, Zygmunt Nita, Krystyna Rybka, The usefulness of light sources based on diodes characterized by a continuous spectrum of white light enriched with a blue band in cereal breeding , Bulletin of Plant Breeding and Acclimatization Institute: No. 284 (2018): Regular issue
- Janusz Kozdój, Dariusz R. Mańkowski, Sylwia Oleszczuk, Analysis of the potential for yield formation in doubled haploids of spring barley (Hordeum vulgare L.) obtained via androgenesis , Bulletin of Plant Breeding and Acclimatization Institute: No. 256 (2010): Regular issue
- Patrycja Siedlarz, Piotr Stefański, Przemysław Matysik, Zygmunt Nita, Krystyna Rybka, The effect of different LED illuminators on the germination index of wheat grains obtained in the greenhouse stage of the SSD breeding process , Bulletin of Plant Breeding and Acclimatization Institute: No. 282 (2017): Regular issue
- Janusz Kozdój, Dariusz R. Mańkowski, Henryk J. Czembor, The analysis of grain yield of spring barley (Hordeum vulgare L.) infected by powdery mildew (Blumeria graminis f. sp. hordei) , Bulletin of Plant Breeding and Acclimatization Institute: No. 254 (2009): Regular issue
- Wojciech Rybiński, Katarzyna Pankiewicz, Jan Bocianowski, Michał Rębarz, Analysis of some traits on phenotypic and molecular level for two- and multi-rowed doubled haploids of spring barley (Hordeum vulgare L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 249 (2008): Regular issue
- Urszula Piechota, Grzegorz Czajowski, Paweł Czembor, Evaluation of the barley landraces resistance to powdery mildew (Blumeria graminis) , Bulletin of Plant Breeding and Acclimatization Institute: No. 297/298 (2022): Regular issue
- Urszula Piechota, Paweł Czembor , Barley and Blumeria graminis. Introduction to the host – pathogen interaction , Bulletin of Plant Breeding and Acclimatization Institute: No. 289 (2020): Regular issue
- Renata Orłowska, Katarzyna Pachota, Joanna Machczyńska, Agnieszka Niedziela, Janusz Zimny, Piotr T. Bednarek, Application of the Taguchi method in the androgenesis efficiency enhancment in cereal tissue cultures , Bulletin of Plant Breeding and Acclimatization Institute: No. 287 (2019): Special issue
- Iwona Żur, Ewa Dubas, Sabina Malaga, Franciszek Janowiak, Anna Janeczko, Marcin Rapacz, Tomasz Hura, Piotr Waligórski, Agnieszka Ostrowska, Magdalena Wójcik-Jagła, Identification of factors which determine drought and freezing tolerance in barley (Hordeum vulgare L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
You may also start an advanced similarity search for this article.
