Determination of optimal sampling time for detection of BNYVV by ELISA in sugar beet plants from fields suspected of rhizomania occurrence
Jacek Piszczek
sekretariat@iorpib.poznan.plInstytut Ochrony Roślin w Poznaniu, Terenowa Stacja Doświadczalna w Toruniu (Poland)
Małgorzata Jeżewska
Instytut Ochrony Roślin w Poznaniu (Poland)
Abstract
Climatic conditions in Poland are mostly unfavourable for development of characteristic rhizomania symptoms in sugar beet plants. In most cases, the limited growth and leaf yellowing are the only symptoms found in plants infected with BNYVV. Such symptoms are often confused with those caused by poor soil quality or water deficit. The aim of this work was to determine the optimal period for BNYVV diagnostics in plants originating from the fields suspected of rhizomania infestation. Samples were taken four times (III decade of July, II and III decade of August, II decade of September 2000) from six fields located near the sites on which the occurrence of BNYVV was recorded in earlier years. The samples for ELISA were extracts from root, plant heart, and the oldest leaves. Bio-Rad products were used in the test. It was found that a frequency of positive readings increased from 65% in the first term of sampling up to 100% in the last one.
Keywords:
rhizomania, sampling term, sugar beetReferences
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Authors
Jacek Piszczeksekretariat@iorpib.poznan.pl
Instytut Ochrony Roślin w Poznaniu, Terenowa Stacja Doświadczalna w Toruniu Poland
Authors
Małgorzata JeżewskaInstytut Ochrony Roślin w Poznaniu Poland
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Copyright (c) 2005 Jacek Piszczek, Małgorzata Jeżewska
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