The chromosomal location of markers of aluminium tolerance in rye
Iwona Wiśniewska
i.wisniewska@ihar.edu.plZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Andrzej Rafalski
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Abstract
The hybridization to DNA of the set of wheat-rye addition lines is the technique commonly used for chromosomal location of DNA based markers in rye. The other approach is the hybridization to the DNA of available set of substitution lines of triticale. Because of the tendency to spontaneous loss of alien chromosomes in wheat-rye addition lines, their presence was verified by Southern dot-blot hybridization using two DNA probes characteristic of rye specific dispersed repetitive sequence. Both probes revealed identical or similar hybridization signals during the analysis of DNA obtained from 103 single seedlings of wheat-rye addition lines. These results confirmed the total or partial loss of rye DNA in some seedlings. The DNA of single seedlings which showed strong and uniform hybridization signal was selected for chromosomal location of rye fragments. The DNA of the set of D/R substitution lines of triticale was also included in these experiments. Four of 18 fragments used as the probes showed increase of hybridization signal to DNA of 4R addition line of wheat with simultaneous decrease of the signal to DNA of 4R/4D substitution line of triticale. The hybridization pattern of the remaining fragments was not informative because of similar intensity of signals to all additions and substitutions or of not consistent differences in intensity of signals to some additions and substitutions.
Keywords:
aluminium tolerance, chromosomal location, hybridization, markers, ryeReferences
Anamthawat-Jonsson K., Schwarzacher T., Leitch A. R., Bennett M. D., Heslop-Harrison J. S. 1990. Discrimination between closely related Triticeae species using genomic DNA as a probe. Theor. Appl. Genet. 79: 721 — 728.
DOI: https://doi.org/10.1007/BF00224236
Google Scholar
Anioł A.1981. Metody określania tolerancyjności zbóż na toksyczne działanie jonów glinu. Biul. IHAR 143: 3 — 14.
Google Scholar
Davis L. G., Dibner M. D., Battey J. F. 1986. Basic methods in molecular biology. Elsevier Sci. Publ., New York: 42 — 43.
DOI: https://doi.org/10.1016/B978-0-444-01082-7.50018-7
Google Scholar
Gallego F. J., Lopez-Solanilla E., Figueiras A. M., Benito C. 1998. Chromosomal location of PCR fragments as a source of DNA markers linked to aluminium tolerance genes in rye. Theor. Appl. Genet. 96: 426 — 434.
DOI: https://doi.org/10.1007/s001220050759
Google Scholar
Konieczny A., Ausubel F. A. 1993. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. The Plant Journal 4: 403 — 410.
DOI: https://doi.org/10.1046/j.1365-313X.1993.04020403.x
Google Scholar
Łukaszewski A. J. 1988. A comparison of several approaches in the development of disomic alien addition lines of wheat. Proc. 7th Int. Wheat Genet. Symp. Cambridge, England: 363 — 367.
Google Scholar
Metzlaff M., Troebner W., Baldauf F., Schlegel R., Cullum J. 1986. Wheat specific repetitive DNA sequences — construction and characterisation of four different genomic clones. Theor. Appl. Genet. 72: 207 — 210.
DOI: https://doi.org/10.1007/BF00266993
Google Scholar
Michelmore, R. W., Paran, I., Kessell, R. V. 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segreganting populations. Proc. Natl. Acad. Sci. USA 88: 9828 — 9832.
DOI: https://doi.org/10.1073/pnas.88.21.9828
Google Scholar
Miftahudin, Scoles G. J., Gustafson, J. P. 2002. AFLP markers tightly linked to the aluminium-tolerance gene Alt3 in rye (Secale cereale L.). Theor. Appl. Genet. 104: 626 — 631.
DOI: https://doi.org/10.1007/s00122-001-0782-3
Google Scholar
Rafalski A., Wiśniewska I., Anioł A. 1996. Screening for molecular markers of aluminium tolerance in rye. J. Appl. Genet. 37A: 79 — 81.
Google Scholar
Rafalski A., Wiśniewska I., Sikora T., Łapiński B. 2000. Molecular probes for detection of wheat chromosomal fragments in rye. Plant Breeding and Seed Science 44: 27 — 38.
Google Scholar
Rafalski A., Madej L., Wiśniewska I., Gaweł M. 2002. The genetic diversity of components of rye hybrids. Cell. Mol. Biol. Lett. 7: 471 — 475.
Google Scholar
Rogowsky P. M., Shepard K. W., Langridge P. 1992. Polymerase chain reaction mapping of rye involving repeated DNA sequences. Genome 35: 621 — 626.
DOI: https://doi.org/10.1139/g92-093
Google Scholar
Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. 1985. Enzymatic amplification of beta-globin genomic sequences and restriction analysis of sickle-cell anemia. Science 230: 1350 — 1354.
DOI: https://doi.org/10.1126/science.2999980
Google Scholar
Authors
Iwona Wiśniewskai.wisniewska@ihar.edu.pl
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Andrzej RafalskiZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Statistics
Abstract views: 62PDF downloads: 29
License
Copyright (c) 2003 Iwona Wiśniewska, Andrzej Rafalski
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Andrzej Rafalski, Magdalena Żurawska, Irena Kolasińska, Iwona Wiśniewska, The molecular analysis of male-sterile, maintainer and restorer lines of rye , Bulletin of Plant Breeding and Acclimatization Institute: No. 235 (2005): Regular issue
- Andrzej Rafalski, Małgorzata Gaweł, Iwona Wiśniewska, The analysis of genetic diversity of components of rye hybrids , Bulletin of Plant Breeding and Acclimatization Institute: No. 230 (2003): Regular issue
- Andrzej Rafalski, Iwona Wiśniewska, Krzysztof Klimont, The changes of amplified DNA fragments profiles after laser irradiation of pea and been seeds , Bulletin of Plant Breeding and Acclimatization Institute: No. 220 (2001): Regular Issue
- Andrzej Rafalski, Iwona Wiśniewska, Krzysztof Klimont, The changes of amplified DNA fragment profiles after laser irradiation of barley seeds , Bulletin of Plant Breeding and Acclimatization Institute: No. 218/219 (2001): Regular Issue
- Iwona Wiśniewska, Andrzej Rafalski, Lucjan Madej, The analysis of genetic diversity of rye inbred lines and their uniformity , Bulletin of Plant Breeding and Acclimatization Institute: No. 218/219 (2001): Regular Issue
