Evaluation of stabillity of triticale traits using electrophoretical analysis of seed proteins
Jerzy Drzewiecki
j.drzewiecki@ihar.edu.plZakład Nasiennictwa i Nasionoznawstwa, Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)
Elżbieta Małuszyńska
Zakład Nasiennictwa i Nasionoznawstwa, Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)
Abstract
The 3rd generation of pre-basic seed of winter triticale varieties Bogo and Presto was obtained in three locations (Lublin, Olsztyn and Sandomierz). Seeds of initial material stored in Gene Bank were sown as a control. The third generation of seeds and samples from Gene Bank were examined. The electrophoresis of prolamins and glutenins was performed for seeds collected in each location. The prolamins were extracted from singular seeds and from bulk samples (50 grains milled together). The glutenins were extracted only form singular seeds. It was confirmed that triticale seed storage proteins are heterogenous. Presto was uniform in its electrophoretic patterns of both gliadins and glutenins. Bogo was uniform only in its electrophoretic pattern of glutenin. The both varieties were stable in their electrophoretic patterns, independently on location of reproduction. After three years of reproduction the both examined varieties were identical by gliadin and glutenin patterns to the initial material.
Supporting Agencies
Keywords:
electrophoresis, reproduction, seed proteins, stability of variety, triticaleReferences
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Authors
Jerzy Drzewieckij.drzewiecki@ihar.edu.pl
Zakład Nasiennictwa i Nasionoznawstwa, Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland
Authors
Elżbieta MałuszyńskaZakład Nasiennictwa i Nasionoznawstwa, Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland
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