The analysis of quantitative traits for spring barley mutants obtained with use of the Hordeum bulbosum method
Wojciech Rybiński
office@igr.poznan.plInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Abstract
Seed of two barley cultivars: Maresi (two-rowed) and Klimek (six-rowed) was subjected to mutagenic treatment with N-nitroso-N-methylurea (MNU). The M1 plants were crossed with Hordeum bulbosum and after 14 days the unripened embryos were cultured in vitro until DH plants were obtained. Among the plants mutant genotypes were selected, propagated and used in field experiments during three years. The statistical parameters (means, extremes, coefficients of variation) for the yield structure components showed significantly higher variability among the DH mutants as compared to their initial forms. In the group of two-rowed barleys, the highest significance contrasts between Maresi and its mutants were recorded for the semi-dwarf mutants M53 and M50. These forms were additionally more resistant to lodging and their ripening time was similar to the cultivar Maresi. Among the six-rowed forms, the most significant differences with the cultivar Klimek were found for the short-straw mutants M62 and M63.
Keywords:
Hordeum bulbosum, laser, DH lines, mutants, N-nitroso-N-methylurea, variability of traits, yield structureReferences
Adamski T., Surma M., Jeżowski S. 1995. Badania nad introdukcją cytoplazmy Hordeum bulbosum do wybranych genotypów jęczmienia uprawnego (H. vulgare L.). Hod. Rośl. Aklim. 39: 103 — 110.
Google Scholar
Beversdorf D., Kott L. S. 1987. An in vitro mutagenesis/selection system for Brassica napus. Iowa State J. Res. 61: 435 — 443.
Google Scholar
Chen Y. 1986. “The inheritance of rice pollen plant and its application in crop improvement”, Haploids of Higher Plants in vitro, China Academic Publishers, Beijing: 118 — 136.
Google Scholar
Drozd D., Szajsner H., Laszkiewicz E. 1999. Wykorzystanie biostymulacji laserowej w uprawie pszenicy jarej. Biul. IHAR 211: 85 — 90.
Google Scholar
Gieroba J., Koper R., Matyka S. 1995. The influence of pre-sowing laser biostimulation of maize seed on the crop and nutritive value of the corn. 45th Australian Cereal Chemistry Conference 3: 30 — 35.
Google Scholar
Laib Y., Szarejko I., Polok K., Małuszyński M. 1996. Barley anther culture for doubled haploid mutant production. Mut. Breed. Newsletter 42: 13 — 15.
Google Scholar
Podleśny J. 1997. Wpływ przedsiewnego traktowania nasion światłem laserowym na kształtowanie cech morfologicznych i plonowanie łubinu białego. Łubin we współczesnym rolnictwie, Materiały Konferen-cyjne, Olsztyn-Kortowo: 87 — 92.
Google Scholar
Podleśny J. 1997. Wpływ przedsiewnego traktowania nasion światłem laserowym na kształtowanie się cech morfologicznych i plonowanie bobiku. Zesz. Probl. Post. Nauk Rol. 446: 435 — 439.
Google Scholar
Rybiński W., Patyna H., Przewoźny T. 1993. Mutagenic effect of laser and chemical mutagen in barley (Hordeum vulgare L.) Genet. Pol. 34: 337 — 343.
Google Scholar
Rybiński W. 1998. Wykorzystanie światła lasera w badaniach nad roślinami wyższymi. Zastosowanie kultur in vitro w fizjologii roślin. (red. F. Dubert, M. Rapacz), Kraków: 169 — 179.
Google Scholar
Rybiński W., Jeżowski S., Krajewski P. 2000. Analiza cech ilościowych warunkujących odporność na wyleganie i parametrów struktury plonu u mutantów jęczmienia jarego. Biul. IHAR 216: 229 — 235.
Google Scholar
Rybiński W. 2001. Influence of laser beams combined with chemomutagen (MNU) on the variability of traits and mutation frequency in spring barley. Int. Agrophysics 15: 115 — 119.
Google Scholar
Rybiński W., Pokora L. 2002. Wpływ światła lasera helowo-neonowego i chemomutagenu (MNU) na zmienność cech lędźwianu siewnego (Lathyrus sativus L.) w pokoleniu M1. Acta Agrophysica 62: 127 — 134.
Google Scholar
Surma M. 1997. Wykorzystanie zjawiska eliminacji chromosomów w hodowli jęczmienia. Seminaria Wydziału Rolniczego, AR Poznań 3: 156 — 174.
Google Scholar
Swanson E. B., Herrgesell M. J., Arnoldo M., Sippell D. W., Wong R. S. C. 1989. Microspore mutagenesis and selection. Theor. Appl. Genet. 78: 525 — 530.
DOI: https://doi.org/10.1007/BF00290837
Google Scholar
Szarejko I., Małuszyński M., Polok K., Kiljan A. 1991. Doubled haploids in the mutation breeding of selected crops. Plant Mutation Breeding for Crop Improvement. Proceedings of a Symposium, Vienna 1990, IAEA: 355 — 378.
Google Scholar
Umba Di-Umba, Małuszyński M., Szarejko I., Zbieszczyk J. 1991. High frequency of barley DH — mutants from M1 after mutagenic treatment with MNH and sodium azide. Mut. Breed. Newsletter 38: 8 — 9.
Google Scholar
Zapata F. J., Aldemita R. R. 1989. „Induced of salt tolerance in high-yielding rice varieties through mutagenesis and anther culture”, Current Options for Cereal Improvement. Małuszyński, M. (ed.). Kluwer Academic Publishers, Dordrecht: 193 — 202.
DOI: https://doi.org/10.1007/978-94-009-0893-2_18
Google Scholar
Authors
Wojciech Rybińskioffice@igr.poznan.pl
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Statistics
Abstract views: 150PDF downloads: 28
License
Copyright (c) 2003 Wojciech Rybiński
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Wojciech Rybiński, Jan Bocianowski, Katarzyna Pankiewicz, Variability of morphological and yield-contributing traits of grasspea (Lathyrus sativus L.) mutants , Bulletin of Plant Breeding and Acclimatization Institute: No. 249 (2008): Regular issue
- Wojciech Rybiński, Tadeusz Adamski, Maria Surma, Jan Bocianowski, Obtaining of haploids with use of Hordeum bulbosum method and mutagenesis and analysis of quantitative traits variation in spring barley DH lines , Bulletin of Plant Breeding and Acclimatization Institute: No. 275 (2015): Regular issue
- Katarzyna Pankiewicz, Wojciech Rybiński, Zygmunt Kaczmarek, Estimation of variability on phenotypic and molecular level of spherical-seeded form of grasspea (Lathyrus sativus L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 260/261 (2011): Regular issue
- Wojciech Rybiński, Jan Bocianowski, Szymon Dziamba, Variation of quantitative traits in Polish local grasspea accessions (Lathyrus sativus L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 264 (2012): Regular issue
- Wojciech Rybiński, Jan Bocianowski, Michał Starzycki, Analysis of variability for quantitative traits and chemical composition of seeds of chosen accessions of grass pea (Lathyrus sativus L.) and red pea (Lathyrus cicera L.) with different geographical origins , Bulletin of Plant Breeding and Acclimatization Institute: No. 274 (2014): Regular issue
- Wojciech Rybiński, Michał Starzycki, Robert Rusinek, Jan Bocianowski, Bogusław Szot, Variation of legume seed’s chemical composition and resistance to mechanical damage , Bulletin of Plant Breeding and Acclimatization Institute: No. 268 (2013): Regular issue
- Wojciech Rybiński , Wojciech Święcicki , Paweł Barzyk , Estimation of genetic variability and pyramiding of genes controlling agricultural traits of white lupin , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Wojciech Rybiński, Michał Starzycki, Elżbieta Starzycka-Korbas, Jan Bocianowski, Genus Lathyrus — estimation and characterization using the chosen species and collected accessions , Bulletin of Plant Breeding and Acclimatization Institute: No. 283 (2018): Special issue
- Wojciech Rybiński, Katarzyna Pankiewicz, Jan Bocianowski, Michał Rębarz, Analysis of some traits on phenotypic and molecular level for two- and multi-rowed doubled haploids of spring barley (Hordeum vulgare L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 249 (2008): Regular issue
- Wojciech Rybiński, Karolina Krystkowiak, Anetta Kuczyńska, Michał Rębarz, Variability of hull-less barley mutants (Hordeum vulgare L.) at the phenotypic and molecular levels , Bulletin of Plant Breeding and Acclimatization Institute: No. 245 (2007): Regular issue
