The changes of amplified DNA fragments profiles after laser irradiation of pea and been seeds
Andrzej Rafalski
a.rafalski@ihar.edu.plZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Iwona Wiśniewska
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Krzysztof Klimont
Zakład Nasiennictwa i Nasionoznawstwa, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Abstract
The seeds of two varieties of bean (Phaseolus vulgaris L.), Nida and Prosna and two varieties of pea (Pisum sativum L. ssp. sativum) were irradiated with three doses of laser radiation. The semirandom primers targeting semiconservative sequences of intron-exon junction of plant genes were used for PCR analysis of DNA prepared from seedlings of these varieties. The comparison of DNA amplification profiles revealed polymorphism between control and seedlings from the laser treated seeds. The results obtained indicate, that the irradiation of seeds with increased doses of laser radiation generated some changes of DNA structure, which were maintained also during vegetative growth of plants. The durability of some changes on the DNA level suggests mutagenic effects of laser radiation.
Supporting Agencies
Keywords:
PCR, bean, laser radiation, pea, seedsReferences
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Authors
Andrzej Rafalskia.rafalski@ihar.edu.pl
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Iwona WiśniewskaZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Krzysztof KlimontZakład Nasiennictwa i Nasionoznawstwa, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
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