The changes of amplified DNA fragment profiles after laser irradiation of barley seeds
Andrzej Rafalski
a.rafalski@ihar.edu.plZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Iwona Wiśniewska
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Krzysztof Klimont
Zakład Nasiennictwa i Nasionoznawstwa, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Abstract
The seeds of two varieties of barley (Horedeum vulgare L.), Klimek and Rataj were irradiated with three doses of laser radiation. The semi-specific primers targeting consensus sequences of intron-exon junction of plant genes were used for PCR analysis of DNA prepared from seedlings of these varieties. The comparison of amplification profiles of DNA fragments of both varieties revealed distinct polymorphism between control seedlings and seedlings from the laser treated seeds. The obtained results indicate, that the irradiation of seeds generates some changes of DNA structure, which appear during vegetative growth of plants. The persistence of some changes on DNA level suggests the mutagenic effects of laser radiation.
Supporting Agencies
Keywords:
PCR, barley, seeds, laser radiationReferences
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Authors
Andrzej Rafalskia.rafalski@ihar.edu.pl
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Iwona WiśniewskaZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Krzysztof KlimontZakład Nasiennictwa i Nasionoznawstwa, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
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