Identity of triticale off-type plants on the basis of electrophoretic and cytogenetic analysis of kernels

Elżbieta Małuszyńska

e.maluszynska@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)

Jerzy Drzewiecki


Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)

Bogusław Łapiński


Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)

Abstract

The 2nd, 3rd and 4th multiplications of pre-basic seed of winter triticale varieties Bogo and Presto were conducted in three locations during 3 years (1998–2000). In each location plants were observed on 8 field plots of 1m2 size. Off-type plants were selected which did not fit description of a variety, according to the DUS requirements. Kernels of the randomly selected plants were subjected to cytogenetic and electrophoretic examinations. Chromosome number was determined in seedling roots of 53 germinated kernels and only 7 aneuploids were detected. The occurrence of additional bands in prolamin electrophoregrams was revealed for most of the examined kernels. It suggests that aneuploidy is not the main cause of occurrence of non-typical triticale plants. It could be attributed rather to other factors like cross-pollination or contamination with other varieties’ seed.

Supporting Agencies

The work was carried out as part of research project no. 5P06B04915 financed by the State Committee for Scientific Research (KBN).

Keywords:

aneuploids, electrophoresis, triticale, prolamins, off-type plant

COBORU. 1996. Metodyka badania odrębności, wyrównania i trwałości (OWT) — pszenżyto (X Triticosecale Wittm.) COBORU, Słupia Wielka.
Google Scholar

Borys J. 1997. Zależność wielkości frakcji odmian nie spełniających kryterium wyrównania od parametrów testu statystycznego (dla wybranych gatunków roślin samopylnych). Wiad. Odmian. 65: 15 — 24.
Google Scholar

Brzeziński W., van Gelder W.M.J., Mendelewski P., Kolster P. 1989. Polyacrylamide gel electrophoresis of wheat gliadins: the use of a moving boundary for improved resolution. Euphytica 40: 207 — 212.
Google Scholar

Guedes-Pinto H., Rangel-Figueiredo T., Carnide O. 1984. Aneuploidy in high yielding 6x-triticales. Cereal Res. Commun. 12, 3/4,: 229 — 235.
Google Scholar

Łukaszewski A. J., Xitang Xu. 1995. Screening large populations of wheat hybrids by C-banding. Cereal Res. Commun. 23: 9 — 13.
Google Scholar

Maćkowiak W., Paizert K., Mazurkiewicz L., Woś H. 1993. Osiągnięcia i problemy hodowli pszenżyta w Polsce. Biul. IHAR 187: 143 — 166.
Google Scholar

Pickett. A. A. 1988. Factors affecting seed production of triticale (X Triticosecale Wittm.) Plant Varieties Seeds 1: 63 — 74.
Google Scholar

Rogalska S., Cybulska-Augustyniak J., Kulawinek A. 1991. Aneuploidy in Polish cultivars of winter triticale. Genet. Pol. 32, 1/2: 11 — 16.
Google Scholar

Sowa Wł., Krysiak H. 1994. Ocena odmian pszenżyta pod względem skłonności do obcozapylania. Biul. IHAR 192: 23 — 28.
Google Scholar

Tarkowski Cz. (red.). 1989. Biologia pszenżyta. PWN, Warszawa.
Google Scholar

Wolski T. 1993. Pszenżyto ozime hodowli Danko w Europie. Biul. IHAR 187: 139 — 142.
Google Scholar


Published
2001-09-28

Cited by

Małuszyńska, E., Drzewiecki, J. and Łapiński, B. (2001) “Identity of triticale off-type plants on the basis of electrophoretic and cytogenetic analysis of kernels”, Bulletin of Plant Breeding and Acclimatization Institute, (218/219), pp. 251–259. doi: 10.37317/biul-2001-0058.

Authors

Elżbieta Małuszyńska 
e.maluszynska@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland

Authors

Jerzy Drzewiecki 

Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland

Authors

Bogusław Łapiński 

Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland

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