Identity of triticale off-type plants on the basis of electrophoretic and cytogenetic analysis of kernels
Elżbieta Małuszyńska
e.maluszynska@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)
Jerzy Drzewiecki
Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)
Bogusław Łapiński
Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie (Poland)
Abstract
The 2nd, 3rd and 4th multiplications of pre-basic seed of winter triticale varieties Bogo and Presto were conducted in three locations during 3 years (1998–2000). In each location plants were observed on 8 field plots of 1m2 size. Off-type plants were selected which did not fit description of a variety, according to the DUS requirements. Kernels of the randomly selected plants were subjected to cytogenetic and electrophoretic examinations. Chromosome number was determined in seedling roots of 53 germinated kernels and only 7 aneuploids were detected. The occurrence of additional bands in prolamin electrophoregrams was revealed for most of the examined kernels. It suggests that aneuploidy is not the main cause of occurrence of non-typical triticale plants. It could be attributed rather to other factors like cross-pollination or contamination with other varieties’ seed.
Supporting Agencies
Keywords:
aneuploids, electrophoresis, triticale, prolamins, off-type plantReferences
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Authors
Elżbieta Małuszyńskae.maluszynska@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland
Authors
Jerzy DrzewieckiInstytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland
Authors
Bogusław ŁapińskiInstytut Hodowli i Aklimatyzacji Roślin w Radzikowie Poland
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