Pyramiding of resistance genes against Phytophthora infestans in potato
Jarosław Plich
j.plich@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie (Poland)
Beata Tatarowska
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie (Poland)
Dorota Milczarek
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie (Poland)
Bogdan Flis
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie (Poland)
Abstract
Pyramiding of the resistance genes against P. infestans enhances effectiveness and durability of resistance used in potato breeding programs. The aim of presented study was to combine two broad-spectrum resistance genes against P. infestans. The resistance donors cultivar Bzura (possessing R2-like gene) and potato clone 04-IX-21 (possessing Rpi-phu1 gene) were crossed, and unselected progeny was developed. The resistance of progeny clones was tested in three consecutive years in detached leaflet tests with the use of three groups of isolates different in terms of virulence profiles. Based on the results of phenotypical tests, a group of progeny clones combining both resistance genes were selected. Since phenotypical tests are very laborious and time-consuming, authors proposed selection of such clones with the use of DNA markers specific for these genes. Results of genotyping were fully consistent with phenotypic tests, which confirms that proposed DNA markers can be used for selection of resistant clones in breeding programs.
Keywords:
gene Rpi-phu1, gene R2-like, late blight, R genesReferences
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Authors
Jarosław Plichj.plich@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie Poland
Authors
Beata TatarowskaInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie Poland
Authors
Dorota MilczarekInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie Poland
Authors
Bogdan FlisInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Oddział w Młochowie Poland
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