Assessment of growth and genetic stability of gooseberry (Ribes grossularia L.)propagated in vitro and ex vitro
Danuta Kucharska
danuta.kucharska@inhort.plInstytut Ogrodnictwa, Zakład Biologii Stosowanej, ul. Konstytucji 3 Maja 1/3 96‒100 Skierniewice (Poland)
http://orcid.org/0000-0002-9635-967X
Danuta Wójcik
Instytut Ogrodnictwa, Zakład Biologii Stosowanej, ul. Konstytucji 3 Maja 1/3 96‒100 Skierniewice (Poland)
https://orcid.org/0000-0002-3556-8080
Aleksandra Trzewik
Instytut Ogrodnictwa, Zakład Biologii Stosowanej, ul. Konstytucji 3 Maja 1/3 96‒100 Skierniewice (Poland)
https://orcid.org/0000-0003-0378-5440
Abstract
The aim of the study was to evaluate phenotypically and genetically, 15 gooseberry genotypes plants propagated in vitro and ex vitro by cuttings grown in the second year in field conditions. The outcome of this was that in vitro propagated shrubs were shown to grew more strongly. Their height and the number of shoots were significantly higher for the 11 genotypes and a width of 12 genotypes for gooseberry. However, fruit abundance was greater in much more traditionally multiplied plants. Beyond the aforementioned, Genetic stability analysis of clones derived from in vitro cultures of five gooseberry varieties was performed. Herein, 13-15 plants with in vitro and mother plants were analyzed. The number of products generated by the AFLP primer pairs ranged from 33 to 108. The highest total number of amplification products was obtained as a result of the AFLP reaction for the plants ‚Hinnonmaki Rot’ (300), and the lowest for the varieties ‚Hinsel’ and ‚Resika’ (262). Genetic variability in gooseberry in vitro plants ranged from 1.03% for ‚Captivator’, to 10.3% for ‚Hinsel’. Genetic stability was also assessed using ISSR markers. Plants of five genotypes derived from conventional and in vitro reproduction were used. Accordingly, a total of 2294 amplification products were obtained, of which 2.8% were polymorphic. The size of the obtained products was from 250 to 2900 bp, depending on the starter and variety. ISSR-PCR analysis showed different degrees of polymorphism - from 0 for the ‘Hinnonmaki Rot’ and ‘Resica’ to 11.6% for the ‚Hinsel’ variety.
Supporting Agencies
Keywords:
AFLP, gooseberry, DNA, ISSR, in vitro cultures, polymorphismReferences
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Authors
Danuta Kucharskadanuta.kucharska@inhort.pl
Instytut Ogrodnictwa, Zakład Biologii Stosowanej, ul. Konstytucji 3 Maja 1/3 96‒100 Skierniewice Poland
http://orcid.org/0000-0002-9635-967X
Authors
Danuta WójcikInstytut Ogrodnictwa, Zakład Biologii Stosowanej, ul. Konstytucji 3 Maja 1/3 96‒100 Skierniewice Poland
https://orcid.org/0000-0002-3556-8080
Authors
Aleksandra TrzewikInstytut Ogrodnictwa, Zakład Biologii Stosowanej, ul. Konstytucji 3 Maja 1/3 96‒100 Skierniewice Poland
https://orcid.org/0000-0003-0378-5440
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