Analysis of genome regions linked to the resistance of blackcurrant (Ribes nigrum) to blackcurrant gall mite (Cecidophyopsis ribis)
Anita Kuras
anita.kuras@inhort.plZakład Hodowli Roślin Ogrodniczych, Instytut Ogrodnictwa, ul. Konstytucji 3 Maja 1/3, 96‒100 Skierniewice (Poland)
http://orcid.org/0000-0003-4724-788X
Bogumiła Badek
Zakład Hodowli Roślin Ogrodniczych, Instytut Ogrodnictwa, ul. Konstytucji 3 Maja 1/3, 96‒100 Skierniewice (Poland)
http://orcid.org/0000-0002-0542-0798
Abstract
Blackcurrant gall mite (Cecidophyopsis ribis) is one of the most dangerous pests of blackcurrant. It causes deformation of blackcurrant buds and reduces fruit yield. The selection of the most favorable molecular markers coupled to resistance to C.ribis, can accelerate the breeding process and improve efficacy when choosing genotypes that are resistant to this disease. The aim of this study was to analyze genome regions conjugated with resistance of blackcurrant (Ribes nigrum) to blackcurrant gall mite by assessing the degree of polymorphism in coupling groups, including the Ce and P regions, and by searching for new genome fragments regulating C. ribis resistance. As a result of the analysis of electrophoregrams with CAPS reaction products, 119 polymorphic DNA fragments were identified, out of which 100 were selected for sequence analysis. As the end product of sequencing, 9 specific readings were obtained on DNA matrices of the varieties ‘Ceres’, ‘Ojebyn’, ‘Vir’, ‘Ores’, ‘Foxendown’, ‘Ben Finlay’ and ‘Bona’.
Supporting Agencies
Keywords:
CAPS, Cecidophyopsis ribis, molecular marker, blackcurrantReferences
Anderson, M. M. (1971). Resistance to gall mite (Phytoptus ribis Nal.) in the Eucoreosma section of Ribes. Euphytica (20), 422–426.
Google Scholar
Bang, H., Kim, S., Leskovar, D., King, S. (2007). Development of a codominant CAPS marker for allelic selection between canary yellow and red watermelon based on SNP in lycopene β-cyclase (LCYB) gene. Molecular Breedin (20), 63–72
Google Scholar
Brennan, R., Jorgensen, L., Gordon, S., Loades, K., Hackett, C., Russell, J. (2008). The development of a PCR-based marker linked to resistance to the blackcurrant gall mite (Cecidophyopsis ribis Acari: Eriophyidae). Theor. Appl. Genet. (118), 205–211.
Google Scholar
Brennan, R. M., Lanham, P. G., McNicol, R. J. (1993). Ribes reeding and research in the UK. Acta Horticulturae (352), 267–275. https://doi.org/10.17660/ActaHortic.1993.352.38.
Google Scholar
Doyle, J. J., Doyle, J. L. (1990). Isolation of plant DNA from fresh tissue. Focus (12),13–15.
Google Scholar
Gupta, P. K., Varshney, R. K., Sharma, P. C., Ramesh, B. (1999). Molecular markers and their applications in wheat breeding. Plant Breed (118),369–390.
Google Scholar
Knight, R. L., Keep, E., Briggs, J. B., Parker, J. (1974). Transference of resistance to black currant gall mite Cecidophyopsis ribis, from gooseberry to black currant. Ann. Appl. Biol. (76),123–130.
Google Scholar
Łabanowska, B. H., Piotrowski, W., Gruchała, M. (2015). Blackcurrant gall mite – Cecidophyopsis ribis (Westw.), harmfulness and possibility to control with fenpyroximate (Ortus 05 SC). Prog. Plant Prot. 55 (3), 305–311.
Google Scholar
Mazeikiene, I., Bendokas, V., Stanys, V., Siksnianas, T. (2012): Molecular markers linked to resistance to the gall mite in blackcurrant. Plant Breeding. (131),762–766.
Google Scholar
Pluta, S., Żurawicz, E. (2002). Effect of reversion virus on the yield and fruit size in blackcurrant Ribes nigrum L. Proceedings 8th International Symposium Rubus and Ribes. Scotland, Dundee. July 2001. Acta Horticulturae 585 (1): 393–398.
Google Scholar
Sanger, F., Coulson, A. R. (1975). A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol. 94(3): 441–448.
Google Scholar
Thiel, T, Kota, R, Grosse, I, Stein, N, Graner, A. (2004). SNP2CAPS: a SNP and INDEL analysis tool for CAPS marker development. Nucleic Acids Res; (32),e5.
Google Scholar
Authors
Anita Kurasanita.kuras@inhort.pl
Zakład Hodowli Roślin Ogrodniczych, Instytut Ogrodnictwa, ul. Konstytucji 3 Maja 1/3, 96‒100 Skierniewice Poland
http://orcid.org/0000-0003-4724-788X
Authors
Bogumiła BadekZakład Hodowli Roślin Ogrodniczych, Instytut Ogrodnictwa, ul. Konstytucji 3 Maja 1/3, 96‒100 Skierniewice Poland
http://orcid.org/0000-0002-0542-0798
Statistics
Abstract views: 168PDF downloads: 156 PDF downloads: 31
License
Copyright (c) 2020 Anita Kuras
![Creative Commons License](http://i.creativecommons.org/l/by-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Marek Szymajda, Anita Kuras, Edward Żurawicz, Influence of selected parental forms on fruit and seed set in distant hybridization of three fruit tree species of the genus Prunus (Japanese plum, apricot, myrobalan plum) , Bulletin of Plant Breeding and Acclimatization Institute: No. 291 (2020): Special issue
- Agnieszka Masny, Danuta Kucharska, Monika Mieszczakowska-Frąc, Anita Kuras, Edward Żurawicz, The research on the possibility of extending the genetic variability of red raspberry (Rubus idaeus) in terms of different ripening time and fruit quality , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Bogumiła Badek, Stanisław Pluta, Małgorzata Korbin, Characteristics of molecular markers linked with the resistance to gall mite (Cecidophyopsis ribis) , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Marek Szymajda, Bogusława Idczak, Anita Kuras, Małgorzata Korbin, Edward Żurawicz, Distant hybridization of the Prunus cerasifera (myrobalan plum), Prunus armeniaca (apricot), Prunus salicina (Japanese plum), Prunus domestica (European plum) species for extending genetic biodiversity within the genus Prunus , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Agnieszka Masny, Monika Mieszczakowska-Frąc, Anita Kuras, Edward Żurawicz, Małgorzata Korbin, Studies on possibilities of increasing of bioactive compounds in strawberry fruits through intra- and interspecific hybridization within Fragaria genus , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
Similar Articles
- Ewa Zimnoch-Guzowska, Małgorzata Gołębiewska, Exploitation of biotechnology by Polish plant breeding , Bulletin of Plant Breeding and Acclimatization Institute: No. 259 (2011): Regular issue
- Katarzyna Pankiewicz, Wojciech Rybiński, Zygmunt Kaczmarek, Estimation of variability on phenotypic and molecular level of spherical-seeded form of grasspea (Lathyrus sativus L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 260/261 (2011): Regular issue
- Karolina Krystkowiak, Tadeusz Adamski, Maria Surma, Zygmunt Kaczmarek, Anetta Kuczyńska, Agata Burtna, Renata Trzeciak, Variability of chosen technological traits of winter wheat hybrids in relation to composition of HMW glutenin subunits in parental forms , Bulletin of Plant Breeding and Acclimatization Institute: No. 260/261 (2011): Regular issue
- Monika Markiewicz, Lech Michalczuk, Agnieszka Czajka, Beata Kowalska, Piotr Kamiński, Studies on molecular mechanism of clubroot (Plasmodiophora brassicae) in Brassica plants , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Sylwia Keller-Przybyłkowicz, Mariusz Lewandowski, Evaluation of changes in the expression profile of candidate genes in apple rootstockss with a different degree of frost tolerance . , Bulletin of Plant Breeding and Acclimatization Institute: No. 291 (2020): Special issue
- Mariusz Lewandowski, Sylwia Keller-Przybyłkowicz, Małgorzata Korbin, Edward Żurawicz, Genetic and molecular analysis of selected apple genotypes (Malus domestica) for shortening of the juvenile period and quality improvement of the fruits , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Aleksandra Pietrusińska, Jerzy H. Czembor, Gene pyramiding — a tool commonly used in breeding programs breeding programs , Bulletin of Plant Breeding and Acclimatization Institute: No. 278 (2015): Regular issue
- Dominika Boguszewska-Mańkowska, Potato soil drought resistance and assessment methods , Bulletin of Plant Breeding and Acclimatization Institute: No. 279 (2016): Regular issue
- Magdalena Żurawska-Zajfert, Katarzyna Grelewska-Nowotko, Ewelina Żmijewska, Anna Linkiewicz, Jarosław Nowosielski, Sławomir Sowa, The importance of validation for detection and quantification methods of genetically modified organisms for the purpose of GMO control in the European Union , Bulletin of Plant Breeding and Acclimatization Institute: No. 280 (2016): Regular issue
- Beata Tatarowska, Dorota Milczarek, Jarosław Plich, Bogdan Flis, The reaction of tetraploid potato clones to Potato virus M (PVM) infection , Bulletin of Plant Breeding and Acclimatization Institute: No. 280 (2016): Regular issue
You may also start an advanced similarity search for this article.