Analysis of genome regions linked to the resistance of blackcurrant (Ribes nigrum) to blackcurrant gall mite (Cecidophyopsis ribis)
Anita Kuras
anita.kuras@inhort.plZakład Hodowli Roślin Ogrodniczych, Instytut Ogrodnictwa, ul. Konstytucji 3 Maja 1/3, 96‒100 Skierniewice (Poland)
http://orcid.org/0000-0003-4724-788X
Bogumiła Badek
Zakład Hodowli Roślin Ogrodniczych, Instytut Ogrodnictwa, ul. Konstytucji 3 Maja 1/3, 96‒100 Skierniewice (Poland)
http://orcid.org/0000-0002-0542-0798
Abstract
Blackcurrant gall mite (Cecidophyopsis ribis) is one of the most dangerous pests of blackcurrant. It causes deformation of blackcurrant buds and reduces fruit yield. The selection of the most favorable molecular markers coupled to resistance to C.ribis, can accelerate the breeding process and improve efficacy when choosing genotypes that are resistant to this disease. The aim of this study was to analyze genome regions conjugated with resistance of blackcurrant (Ribes nigrum) to blackcurrant gall mite by assessing the degree of polymorphism in coupling groups, including the Ce and P regions, and by searching for new genome fragments regulating C. ribis resistance. As a result of the analysis of electrophoregrams with CAPS reaction products, 119 polymorphic DNA fragments were identified, out of which 100 were selected for sequence analysis. As the end product of sequencing, 9 specific readings were obtained on DNA matrices of the varieties ‘Ceres’, ‘Ojebyn’, ‘Vir’, ‘Ores’, ‘Foxendown’, ‘Ben Finlay’ and ‘Bona’.
Supporting Agencies
Keywords:
CAPS, Cecidophyopsis ribis, molecular marker, blackcurrantReferences
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Authors
Anita Kurasanita.kuras@inhort.pl
Zakład Hodowli Roślin Ogrodniczych, Instytut Ogrodnictwa, ul. Konstytucji 3 Maja 1/3, 96‒100 Skierniewice Poland
http://orcid.org/0000-0003-4724-788X
Authors
Bogumiła BadekZakład Hodowli Roślin Ogrodniczych, Instytut Ogrodnictwa, ul. Konstytucji 3 Maja 1/3, 96‒100 Skierniewice Poland
http://orcid.org/0000-0002-0542-0798
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