ISOLATION, IDENTIFICATION AND PRESERVATION OF PECTINOLYTIC BACTERIA PATHOGENIC TO POTATO
Renata Lebecka
r.lebecka@ihar.edu.plPlant Breeding and Acclimatization Institute – National Research Institute, Młochów Research Center; Platanowa Str.19, 05-831 Młochów, Poland (Poland)
Abstract
Blackleg of potato plants and soft rot of tubers are caused by several species of pectinolytic bacte-ria from genera Pectobacterium and Dickeya. The text describes simple methods of isolating bacteria from symptomatic and symptomless organs of potato plants, their identification using Polymerase Chain Reaction (PCR) and preservation.
Keywords:
Blackleg, Dickeya, Pectobacterium, Polymerase Chain Reaction, selective medium, soft rotReferences
De Boer S. H., Li X., and Ward L. J. 2012. Pectobacterium spp. Associated with bacterial stem rot syndrome of potato in Canada. Phytopathology 102: 937–947.
Google Scholar
Degefu Y., Potrykus M., Golanowska M., Virtanen E., Lojkowska E. 2013. A new clade of Dickeya spp. plays a major role in potato blackleg outbreaks in North Finland. Ann. Appl. Biol. 162(2): 231-241.
Google Scholar
Duarte V., de Boer S. H., Ward L. J., & de Oliveira A. M. R. 2004. Characterization of atypical Erwinia ca-rotovora strains causing blackleg of potato in Brazil. J. Appl. Microbiology 96: 535–545.
Google Scholar
Helias V., Hamon P., Huchet E., van der Wolf J.M., Andrivon D. 2012. Two new effective semiselective crystal violet pectate media for isolation of Pectobacterium and Dickeya. Plant Pathol. 61: 339–345.
Google Scholar
Kang H.W., Kwon S.W., Go S.J. 2003. PCR-based specific and sensitive detection of Pectobacterium caroto-vorum ssp. carotovorum by primers generated from a URP-PCR fingerprinting-derived polymorphic band. Plant Pathol. 52: 127–133.
Google Scholar
Khayi S., Cigna J., Min Chong T., Quêtu-Laurent A., Chan K.-G., Hélias V., Faure D. 2016. Transfer of the potato plant isolates of Pectobacterium wasabiae to Pectobacterium parmentieri sp. nov. Int. J. Syst. and Evol. Micr. 66: 5379–5383.
Google Scholar
Kim M. H., Cho M. S., Kim B. K., Choi H. J., Hahn J. H., Kim C., Kang M. J., Kim S. H., and Park D. S. 2012. Quantitative real-time polymerase chain reaction assay for detection of Pectobacterium wasabiae using YD repeat protein gene-based primers. Plant Dis. 96: 253-257.
Google Scholar
Gardan L., Gouy C., Christen R. & Samson R. 2003. Elevation of three subspecies of Pectobacterium caroto-vorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov. Int. J. Syst. and Evol. Micr. 53: 381–391.
Google Scholar
Oksińska M., Magnucka E., Pietr S. 2016. Fast molecular detection of Pectobacterium atrosepticum, Pecto-bacterium carotovorum subsp. carotovorum, and Dickeya spp. from potato (Solanum tuberosum L.) stem tissues. Wydawnictwo Uniwersytetu Przyrodniczego we Wrocławiu, ISSN 1505-0297, Electronic Jour-nal of Polish Agricultural Universities 19 (4), #09
Google Scholar
Potrykus M., Sledz W., Golanowska M., Slawiak M., Binek A., Motyka A., Zoledowska S., Czajkowski R., Lojkowska E. 2014. Simultaneous detection of major blackleg and soft rot bacterial pathogens in potato by multiplex polymerase chain reaction. Ann. Appl. Biol. 165: 474-487.
Google Scholar
Pritchard L., Humphris S., Saddler G.S., Parkinson N. M., Bertrand V., Elphinstone J.G., and Toth I.K. 2012. Detection of phytopathogens of the genus Dickeya using a PCR primer prediction pipeline for draft bacterial genome sequences. Plant Pathol. 62: 587-596
Google Scholar
Samson R., Legendre J. B., Christen R., Fischer-Le Saux M., Achouak W. & Gardan L. 2005. Transfer of Pectobacterium chrysanthemi (Burkholder et al. 1953) Brenner et al. 1973 and Brenneria paradisiaca to the genus Dickeya gen. nov. as Dickeya chrysanthemi comb. nov. and Dickeya paradisiaca comb. nov. and delineation of four novel species, Dickeya dadantii sp. nov., Dickeya dianthicola sp. nov., Dickeya dieffenbachiae sp. nov. and Dickeya zeae sp. nov. Int. J. Syst. and Evol. Micr. 55 : 1415–1427.
Google Scholar
Slawiak M., van Beckhoven J. R. C. M., Speksnijder A. G. C. L., Czajkowski R., Grabe, G. & van der Wolf J. M. 2009. Biochemical and genetical analysis reveal a new clade of biovar 3 Dickeya spp. strains isolated from potato in Europe. Eur. J. Plant Pathol. 125: 245–261.
Google Scholar
Toth I.K., van der Wolf J.M., Saddler G. et al., 2011. Dickeya species: an emerging problem for potato pro-duction in Europe. Plant Pathol. 60: 385–399
Google Scholar
Tsror L., Erlich O., Lebiush S., Hazanovsky M., Zig U., Slawiak M., Grabe G., van der Wolf J. M., & van de Haar J. J. 2009. Assessment of recent outbreaks of Dickeya sp. (syn. Erwinia chrysanthemi) slow wilt in potato crops in Israel. Eur. J. Plant Pathol. 123: 311–320.
Google Scholar
Tsror L., Erlich O., Hazanovsky M., Ben Daniel B., Zig U., and Lebiush S. 2012. Detection of Dickeya spp. latent infection in potato seed tubers using PCR or ELISA and correlation with disease incidence in commercial field crops under hot-climate conditions. Plant Pathol. 61: 161–168.
Google Scholar
van der Wolf, J.M., de Haan, E.G., Kastelein, P., Krijger, M., de Haas, B.H., Velvis, H., Mendes, O., Kooman-Gersmann M., van der Zouwen, P.S. 2017. Virulence of Pectobacterium carotovorum subsp. brasiliense on potato compared with that of other Pectobacterium and Dickeya species under climatic conditions prevailing in the Netherlands. Plant Pathol. 66(4):571-583.
Google Scholar
van der Wolf J.M., Speksnijder A., Velvis H., van de Haar J., van Doorn J. 2007. Why is Erwinia chrysanthe-mi (Dickeya sp.) taking over? The ecology of a blackleg pathogen. Hannukkala, A, Segerstedt M. New and Old Pathogens of Potato in Changing Climate. Proceedings of the EAPR Pathology Section seminar, 2–6 July 2007, Hattula, Finland.
Google Scholar
Authors
Renata Lebeckar.lebecka@ihar.edu.pl
Plant Breeding and Acclimatization Institute – National Research Institute, Młochów Research Center; Platanowa Str.19, 05-831 Młochów, Poland Poland
Statistics
Abstract views: 121PDF downloads: 70
License
All articles published in electronic form under CC BY-SA 4.0, in open access, the full content of the licence is available at: https://creativecommons.org/licenses/by-sa/4.0/legalcode.pl .