Using SSR markers for assessment genetic diversity and detection drought escape candidate genes in barley lines (Hordeum vulgare L.)
Vahideh Gougerdchi
Department of Agricultural Biotechnology, Faculty of Agriculture, Payame Noor University, Karaj, Alborz, Iran (Iran, Islamic Republic of)
Sara Dezhsetan
sdezhsetan@uma.ac.irGenomics Department; Agricultural Biotechnology Institute Northwest and West (Tabriz), Agricultural Biotechnology Research Institute of Iran (ABRII), Assistant Professor, Dept .of Agronomy & Plant Breeding, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil, Iran (Iran, Islamic Republic of)
Mohammad Ali Ebrahimi
Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran. (Iran, Islamic Republic of)
Behzad Sadeghzadeh
Dryland Agricultural Research Insti. (DARI), Maragheh, Iran. (Iran, Islamic Republic of)
Sona Savari
Department of Agronomy & Plant Breeding, Faculty of Agriculture, University of Tabriz, Iran. (Iran, Islamic Republic of)
Abstract
Assessment of genetic diversity using molecular markers is one of the primary and important steps in breeding programs. In this study, genetic diversity of 52 barley lines evaluated using 68 SSR primer pairs and 47 primer pairs produced clear and polymorphic banding pattern. In general, 153 polymorphic alleles de-tected. The number of observed polymorphic alleles varied from 2 to 9, with an average of 3.26 alleles per locus. Polymorphic Information Content (PIC) ranged from 0.07 to 0.81, with an average of 0.45. In this research, SSR markers differentiated the studied lines efficiently. Using cluster analysis, studied barley lines divided into two groups. Genetic diversity was relatively corresponding with geographical origins, because the lines related to a country somewhat diverged from each other. Two-rowed Iranian and Chinese barleys classified in one subgroup. Also, most six-rowed barleys classified in one subgroup. Association mapping analysis was used to identify candidate genes for drought escape in barley lines and 16 informative markers were identified after which confirmation in other tests could be suitable for marker assisted breeding drought escape.
Keywords:
Association analysis, barley, genetic diversity, Microsatellite markers (SSR), drought escapeReferences
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Authors
Vahideh GougerdchiDepartment of Agricultural Biotechnology, Faculty of Agriculture, Payame Noor University, Karaj, Alborz, Iran Iran, Islamic Republic of
Authors
Sara Dezhsetansdezhsetan@uma.ac.ir
Genomics Department; Agricultural Biotechnology Institute Northwest and West (Tabriz), Agricultural Biotechnology Research Institute of Iran (ABRII), Assistant Professor, Dept .of Agronomy & Plant Breeding, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil, Iran Iran, Islamic Republic of
Authors
Mohammad Ali EbrahimiDepartment of Agricultural Biotechnology, Payame Noor University, Tehran, Iran. Iran, Islamic Republic of
Authors
Behzad SadeghzadehDryland Agricultural Research Insti. (DARI), Maragheh, Iran. Iran, Islamic Republic of
Authors
Sona SavariDepartment of Agronomy & Plant Breeding, Faculty of Agriculture, University of Tabriz, Iran. Iran, Islamic Republic of
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