Development of microsatellite markers in rye: map construction.

B. Hackauf

Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, Rudolf-Schick-Platz 3a, D-18190 Gross Luesewitz, Germany (Germany)

P. Wehling

Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, Rudolf-Schick-Platz 3a, D-18190 Gross Luesewitz, Germany (Germany)

Abstract

Rye ESTs from public sequence databases proved to be a valuable resource to develop micro­satellite markers in rye. One hundred and twenty-one EST-derived Secale cereale microsatellites (SCM) were genetically analyzed in a BC1 population. Fourty-three percent of the studied SCM markers displayed a polymorphism in this mapping population. Linkage analysis in relation to genomic SSR anchor markers as well as AFLP markers allowed to arrange 41 polymorphic EST-derived SCM markers into seven linkage groups which correspond to the seven rye chromosomes. In total, 56 rye SSR markers could be integrated in this second-generation linkage map of rye comprising 685 cM of the rye genome. Distorted segregations with excess of heterozygous progeny were found for most of the markers on chromosome 7R and possible reasons for these are discussed.

Keywords:

distorted segregation, ESTs, functional map microsatellites, Secale cereale L.