CRYOPRESERVATION OF SHOOT TIPS AND POLLEN OF POTATO
Paulina Smyda-Dajmund
p.smyda@ihar.edu.plPlant Breeding and Acclimatization Institute – National Research Institute, Młochów Research Center, Platanowa Str. 19, 05-831 Młochów, Poland (Poland)
Abstract
Cryopreservation is a frequently used method of long-term storage of potato meristems and pollen in liq-uid nitrogen (LN) in temperature of -196°C. This technique allows for theoretically unlimited storage of pota-to material. The most popular method of potato shoot tips preservation is cryopreservation by the solidifica-tion of liquids without crystallization (vitrification).The best method of pollen conservation is its direct im-mersion in LN. The successful regeneration after vitrification is genotype-dependent, which require optimiza-tion of protocol.
Keywords:
meristems, liquid nitrogen, long-term storage, pollen, vitrificationSmyda-Dajmund, P. (2017). CRYOPRESERVATION OF SHOOT TIPS AND POLLEN OF POTATO. Plant Breeding and Seed Science, 76, 75–80. Retrieved from https://ojs.ihar.edu.pl/index.php/pbss/article/view/198
Authors
Paulina Smyda-Dajmundp.smyda@ihar.edu.pl
Plant Breeding and Acclimatization Institute – National Research Institute, Młochów Research Center, Platanowa Str. 19, 05-831 Młochów, Poland Poland
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