Interval mapping of genes controlling growth of rye plants

Paweł Milczarski

University of Agriculture, Department of Plant Breeding, Słowackiego 17, 71-434 Szczecin, Poland (Poland)

Piotr Masojć

University of Agriculture, Department of Plant Breeding, Słowackiego 17, 71-434 Szczecin, Poland (Poland)

Abstract

The F₂-type population derived from the cross between DS2 and RXL1O inbred lines was used for interval mapping of five growth related traits i.e. plant height, spike length, thousand grain weight, kernel length and kernel thickness. Scanning of the whole 1,140 cM length of rye genetic map consisting of 286 marker loci revealed the existence of 6 regions containing QTL5 on chromosomes 1R-5R. Plant height was strongly affected by 1-3 linked dwarfing genes from a distal region of the chromosome 5RL and by 1 gene on the chromosome 3RL, tightly linked to a marker loci Xpsr4 75. These same genes regulated also thousand grain weight and kernel length and thickness. Spike length was determined only by the QTL from chromosome 5RL. In addition a single QTL from chromosome 2R affecting thousand grain weight and kernel thickness was identified, near the molecular marker locus Xrsq8OS. 1. Kernel length and kernel thickness were additionally controlled by QTL5 on chromosomes 2R and 1R and 4R, respectively.

Keywords:

QTLs, growth, genetic map, molecular markers, Secale cereale L.