Male fertility restoration of rye crosses in the pampa cytoplasm.

Irena Kolasiñska

Abstract

Male fertility restoration of F₁ crosses between male sterile lines (P lines) and restorers (R) was studied in 2000. Pollen fertility of 50 F₁ crosses derived by crossing 10 P lines to 5 restorers was estimated. The degree of male sterility/fertility was assessed by visual anther score of single plants on a 1-9 scale, restorer indices and visual pollen shedding scores of plots on a 1-9 scale. @Abstract = Coefficients of correlation between the above fertility traits were calculated. The analysis of variance showed that the degree of male fertility of F₁ crosses strongly depends on a restorer genotype, a P line genotype as well as on the P ´ R interaction. The restorer and P line genotypes were a major source of variation.A considerable range of pollen fertility was evident in the single crosses. The mean restorer index of F₁ crosses ranged from 3,4 to 98,7%. The P lines under study were divided into three groups on the basis of mean restorer indices of their crosses with five restorers: easy, medium and difficult to restore. All the restorers tested, provide a sufficient restoration of male fertility of the F₁ crosses with majority of the P lines. The new restorer, 330 R appeared to be more effective in restoration of pollen fertility than the other currently used lines