The influence of selected cytokinins on micropropagation of Salvia officinalis
Aleksandra Luwańska
sekretariat@iwnirz.plInstytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu, Zakład Biotechnologii (Poland)
Karolina Wielgus
Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu, Zakład Biotechnologii (Poland)
Marlena Szalata
Uniwersytet Przyrodniczy w Poznaniu, Katedra Biochemii i Biotechnologii (Poland)
https://orcid.org/0000-0002-0153-4317
Milena Szalata
Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu, Zakład Biotechnologii (Poland)
Ryszard Słomski
Uniwersytet Przyrodniczy w Poznaniu, Katedra Biochemii i Biotechnologii (Poland)
https://orcid.org/0000-0001-5601-7002
Abstract
Common sage (Salvia officinalis) is a plant with a very wide range of healing properties, from which the most important are disinfecting, anti-inflammatory and antisudoral effects. Sage extracts regulate the digestive system and increase blood sugar level. They are also a strong antiseptic medicine which inactivates bacterial toxins and inhibits reproduction of many Gram-positive and Gram-negative bacteria, including the antibiotic-resistant ones. Sage owes its versatile properties to its essential oil composition containing among others thujone, cineole, camphor, borneol and pinene. Apart from the above, it also contains catechins, triterpene, flavonoids, carnosol and organic acids, vitamins B1, C, PP and carotene. Use of in vitro techniques enables mass propagation of selected plants with the best healing parameters or plants with new features gained through transformation. The aim of the study was the determination of the influence of selected cytokinins on micropropagation efficiency of S. officinalis. Materials for research were apical explants of sage cultivar Bona. The cultivation was conducted on the Murashige Skoog (MS) medium, containing different concentration of kinetin, BAP and zeatin (2, 5, 10 mg/ml). The best plant multiplication rate was obtained by using cytokinin BAP (3.5–3, depending on the concentration), however an excessive percentage of vitrificated plants limited the use of this phytohormone. Effectiveness and stability of the micropropagation using BAP at concentration 0.3 mg/l was verified during long term cultivation (to n-6 subculture). The work also included use of cytokinin meta-Topolin as alternative for BAP phytohormone. The best results in the sage micropropagation process were obtained by the application of medium with 0.3 mg/l of BAP. In this case multiplication rate varies between 2.4 and 3.4 during the successive propagation cycles.
Supporting Agencies
Keywords:
BAP, in vitro, cytokinins, micropropagation, sageReferences
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Authors
Aleksandra Luwańskasekretariat@iwnirz.pl
Instytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu, Zakład Biotechnologii Poland
Authors
Karolina WielgusInstytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu, Zakład Biotechnologii Poland
Authors
Marlena SzalataUniwersytet Przyrodniczy w Poznaniu, Katedra Biochemii i Biotechnologii Poland
https://orcid.org/0000-0002-0153-4317
Authors
Milena SzalataInstytut Włókien Naturalnych i Roślin Zielarskich w Poznaniu, Zakład Biotechnologii Poland
Authors
Ryszard SłomskiUniwersytet Przyrodniczy w Poznaniu, Katedra Biochemii i Biotechnologii Poland
https://orcid.org/0000-0001-5601-7002
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