Application of the Taguchi method in the androgenesis efficiency enhancment in cereal tissue cultures
Renata Orłowska
r.orlowska@ihar.edu.plInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie (Poland)
https://orcid.org/0000-0001-7150-6920
Katarzyna Pachota
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie (Poland)
Joanna Machczyńska
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie (Poland)
https://orcid.org/0000-0003-3383-8443
Agnieszka Niedziela
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie (Poland)
https://orcid.org/0000-0002-2886-8835
Janusz Zimny
Zakład Biotechnologii i Cytogenetyki Roślin (Poland)
https://orcid.org/0000-0002-1521-0874
Piotr T. Bednarek
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie (Poland)
https://orcid.org/0000-0002-1553-8378
Abstract
In cereal tissue cultures, one of the most difficult problems to overcome during the production of doubled haploids (DH) is the albinism. It seems that a quite effective way to reduce albinism is to choose the right stress factor in combination with a balanced composition of medium. In the presented work supplementation of nutrients with copper and silver ions (CuSO4, AgNO3) and modulation of cultures time on induction medium for three cereal species: barley (Hordeum vulgare L.), wheat (Triticum aestivum L.) and triticale (x Triticosecale spp. Wittmack ex A. Camus 1927) were used. Application of the Taguchi method enabled to limit the number of experiments and to optimize the conditions for tissue cultures, which enhanced of the amount of green regenerants in all analyzed species.
Keywords:
Taguchi method, optimization, cereal tissue cultures, CuSO4, AgNO3, induction lengthAuthors
Renata Orłowskar.orlowska@ihar.edu.pl
Instytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie Poland
https://orcid.org/0000-0001-7150-6920
Authors
Katarzyna PachotaInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie Poland
Authors
Joanna MachczyńskaInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie Poland
https://orcid.org/0000-0003-3383-8443
Authors
Agnieszka NiedzielaInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie Poland
https://orcid.org/0000-0002-2886-8835
Authors
Janusz ZimnyZakład Biotechnologii i Cytogenetyki Roślin Poland
https://orcid.org/0000-0002-1521-0874
Authors
Piotr T. BednarekInstytut Hodowli i Aklimatyzacji Roślin — Państwowy Instytut Badawczy, Zakład Biochemii i Fizjologii Roślin, Radzików, 05-870 Błonie Poland
https://orcid.org/0000-0002-1553-8378
Statistics
Abstract views: 219PDF downloads: 150
License
![Creative Commons License](http://i.creativecommons.org/l/by-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Piotr T. Bednarek, Renata Orłowska, Analysis of somaclonal variation induced in cereals tissue cultures , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Renata Orłowska, Piotr T. Bednarek, Sławomir Bany, Molecular characterization of the impact of transposable elements on genetic variation in cereals tissue cultures , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Michał Nowak , Piotr T. Bednarek, Justyna Leśniowska-Nowak , Magdalena Sozoniuk , Karolina Dudziak , Magdalena Kawęcka , Karolina Różaniecka , Identification of genome regions and DNA markers associated with heterosis in hexaploid winter triticale , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Piotr Bednarek, Agnieszka Niedziela, Marzena Wasiak, Sławomir Bany , Identification of pollen fertility restoration markers in rye (Secale cereale L.) with CMS-Pampa , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Piotr T. Bednarek, Marzena Wasiak, Agnieszka Niedziela, Exploration the markers linked to the pollen sterility genes in triticale with CMS Tt , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Weronika Jarska, Agnieszka Niedziela, Renata Orłowska, Piotr T. Bednarek, Molecular strategies in modern plant breeding , Bulletin of Plant Breeding and Acclimatization Institute: No. 275 (2015): Regular issue
- Janusz Zimny, Katarzyna Makowska, Aleksandra Zimny, Andrzej Czaplicki, Sylwia Oleszczuk , The study on rye microspore reaction to stress and in vitro culture conditions , Bulletin of Plant Breeding and Acclimatization Institute: No. 286 (2019): Special issue
- Katarzyna Pachota, Agnieszka Niedziela, Renata Orłowska, Piotr T. Bednarek, Modern methods of genotyping DArT and GBS in agriculture important crops , Bulletin of Plant Breeding and Acclimatization Institute: No. 279 (2016): Regular issue
- Agnieszka Niedziela, Weronika Jarska, Piotr T. Bednarek, Up-to-date solutions in modern plant breeding programs , Bulletin of Plant Breeding and Acclimatization Institute: No. 275 (2015): Regular issue
Similar Articles
- Wiesław Pilarczyk, Ewa Bakinowska, Jan Bocianowski, Bogna Zawieja, Optimization of preliminary plant breeding trials with spring barley , Bulletin of Plant Breeding and Acclimatization Institute: No. 255 (2010): Regular issue
- Tadeusz Śmiałowski, Janusz Burek, A genetic analyses of selected winter rye (Secale cereale L.) populations in regard to the important agricultural traits. Part I. The epistatic effect of the genes , Bulletin of Plant Breeding and Acclimatization Institute: No. 268 (2013): Regular issue
- Grzegorz Czajowski, Katarzyna Karska, Anna Strzembicka, Characteristic of winter triticale breeding lines in terms of the degree of infestation by Puccinia triticina and Blumeria graminis , Bulletin of Plant Breeding and Acclimatization Institute: No. 268 (2013): Regular issue
- Anna Strzembicka, Grzegorz Czajowski, Katarzyna Karska, Characteristics of the winter wheat breeding materials in respect of resistance to leaf rust Puccinia triticina , Bulletin of Plant Breeding and Acclimatization Institute: No. 268 (2013): Regular issue
- Teresa Pastuszewska, Katarzyna Franke, Mirosław Nowakowski, Effect of white mustard on density of potato cyst nematode (Globodera rostochiensis) population in soil , Bulletin of Plant Breeding and Acclimatization Institute: No. 269 (2013): Regular issue
- Cezary Trawczyński, Balance of nitrogen, phosphorus and potassium in the organic crop production system on the light soil , Bulletin of Plant Breeding and Acclimatization Institute: No. 269 (2013): Regular issue
- Elżbieta Czembor, Magdalena Matusiak, Piotr Ochodzki, Resistance of maize hybrids to ear rot caused by Fusarium graminearum and F. verticillioides in Poland in years 2008–2009 , Bulletin of Plant Breeding and Acclimatization Institute: No. 270 (2013): Regular issue
- Magdalena Pol-Szyszko, Piotr Masojč, Izabela Doniec, Agnieszka Wenda, Analysis of α-amylase activity in grain of maize (Zea mays L.) cultivars in respect of their application to bioethanol production , Bulletin of Plant Breeding and Acclimatization Institute: No. 254 (2009): Regular issue
- Zbigniew Czerko, The influence of potato cultivar and storage temperature on the amount of losses in tuber mass , Bulletin of Plant Breeding and Acclimatization Institute: No. 254 (2009): Regular issue
- Magdalena Wiśniewska, Impact of barley grain malting time on malt quality , Bulletin of Plant Breeding and Acclimatization Institute: No. 297/298 (2022): Regular issue
<< < 18 19 20 21 22 23 24 25 26 > >>
You may also start an advanced similarity search for this article.