Genetic similarity of various populations of alfalfa (Medicago sativa L. sl.) and their seed yield potential
Zbigniew Broda
zbroda@up.poznan.plKatedra Genetyki i Hodowli Roślin, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Agnieszka Dobrzycka
Katedra Genetyki i Hodowli Roślin, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Jan Bocianowski
Katedra Metod Matematycznych i Statystycznych, Uniwersytet Przyrodniczy w Poznaniu (Poland)
Abstract
Several traits affecting seed yield were observed in 16 genetically differentiated populations of alfalfa. The analyzed traits included number of pods per infrutescence, number of seeds per pod, seed yield per infrutescence and fertility. An analysis of genetic similarity was carried out for the populations using RAPD markers, in two series. For the first series the DNA was isolated using the Thomson-Henry’s method and for the other series – with the column method. The dendrograms of genetic similarity varied for both methods and included four and five groups of similarity, respectively. Variability of the observed traits was compared between and within the similarity groups using analysis of variance.
Keywords:
alfalfa, seed yield, RAPD-PCRReferences
Brown P. T. H., Lange F. D., Kranz E., Lorz H. 1993. Analysis of single protoplasts and regenerated plants by PCR and RAPD technology. Mol. Gen. Genet. 235: 157 — 165.
Google Scholar
Crochemore M. L., Huyghe C., Kerlan M. C., Durand F., Julier B. 1996. Partitioning and distribution of RAPD variation in a set of populations of the Medicago sativa complex. Agronomie 16: 421 — 432.
Google Scholar
Davin-Regli A., Abed Y., Charrel R. N., Bollet C., Micco P. 1995. Variations in DNA concentrations significantly affect the reproducibility of RAPD fingerprint patterns. Res. Microbiol. 146: 561 — 568.
Google Scholar
Delouche J. C. 1980. Environmental effects on seed development and seed quality. Hort. Sc. 15: 775 — 780.
Google Scholar
Echt C. S., Erdahl L. A., McCoy T. J. 1992. Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa. Genome 35: 84 — 87.
Google Scholar
Genstat 5 Committee. 1993. Genstat 5 Release 3 Reference Manual. Clarendon Press, Oxford.
Google Scholar
Hu J., Quiros C. F. 1991. Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep. 10: 505 — 511.
Google Scholar
Irzykowska L., Bocianowski, J. 2008. Genetic variation, pathogenicity and mycelial growth rate differentiation between Gaeumannomyces graminis var. tritici isolates derived from winter and spring wheat. Annales of Applied Biology 152: 369 — 375.
Google Scholar
Julier B., Porcheron A., Ecalle C., Guy P. 1995. Genetic variability for morphology, growth and forage yield among perennial diploid and tetraploid lucerne populations (Medicago sativa L.). Agronomie 15: 295 — 304.
Google Scholar
Marilla E. F., Scoles G. J. 1996. The use of RAPD markers in Hordeum phylogeny. Genome 39: 646 — 654.
Google Scholar
Meunier J. R., Grimont P. A. D. 1993. Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting. Res. Microbiol. 144: 373 — 379.
Google Scholar
Nei M., Li W. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269 — 5273.
Google Scholar
Park Y. H., Kohel R. J. 1994. Effects of concentration of MgCl2 on RAPD DNA polymorphism. Biotech. 16: 652.
Google Scholar
Penner G. A., Bush A., Wise R., Kim W., Domier L., Kasha K., Laroche A., Scoles G., Molnar S. J., Fedak G. 1993. Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories. PCR Methods Appl. 2: 341 — 345.
Google Scholar
Schachermayr G., Siedler H., Gale M. D., Winzeler H., Winzeler M., Keller B. 1994. Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theor. Appl. Genet. 88: 110 — 115.
Google Scholar
Słomski R. (red.). 2001. Przykłady analiz DNA — praca zbiorowa: 158 — 160.
Google Scholar
Staszewski Z. 1975. Lucerny. PWRiL, Warszawa.
Google Scholar
Sypniewski J. 1986. Problemy uprawy roślin strączkowych w Polsce. Fragm. Agron. 1: 29 — 36.
Google Scholar
Thomson D., Henry R. 1995. Single step protocol for preparation of plant tissue for analysis by PCR. Biotechniques 19: 394 — 400.
Google Scholar
Volenec J. J., Cunningham S. M., Haagenson D. M., Berg W. K., Joern B. C., Wiersma D. W. 2002. Physiological genetics of alfalfa improvement: past failures, future prospects. Field Crops Research 75: 97 — 110
Google Scholar
Waugh R., Powell W. 1992. Using RAPD markers for crop improvement. Trends Biotech. 10: 186 — 191.
Google Scholar
Weder Jurgen K.P., 2002. Identification of food and feed legumes by RAPD-PCR. Lebensm.-Wiss. und Technol. 35: 504 — 511.
Google Scholar
Willams J. G., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531 — 6535.
Google Scholar
Yu K., Pauls K. P. 1993. Rapid estimation of genetic relatedness among heterogeneous populations of alfalfa by random amplification of bulked genomic DNA samples. Theor. Appl. Genet. 86: 788 — 794.
Google Scholar
Authors
Zbigniew Brodazbroda@up.poznan.pl
Katedra Genetyki i Hodowli Roślin, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Agnieszka DobrzyckaKatedra Genetyki i Hodowli Roślin, Uniwersytet Przyrodniczy w Poznaniu Poland
Authors
Jan BocianowskiKatedra Metod Matematycznych i Statystycznych, Uniwersytet Przyrodniczy w Poznaniu Poland
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