Molecular and phenotypic differentiation of wheat (Triticum aestivum L.) varieties and their doubled haploid lines
Katarzyna Pankiewicz
office@igr.poznan.plInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Tadeusz Adamski
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Zygm Kaczmarek
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Maria Surma
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Michał Rębarz
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu (Poland)
Abstract
The aim of this study was to examine homogeneity of several wheat varieties (Triticum aestivum L.) cultivated in Poland. RAPD (Random Amplified Polymorphic DNA) polymorphism was studied in seven wheat cultivars: Batis, Changer, Elena, Kobra, Kornett, Kris and Mikon, and doubled haploid (DH) lines derived from them. Twenty decamer primers were used. Totally, 145 amplification products were observed 107 ones were polymorphic. Genetic similarity between each variety and its DH lines was estimated. Besides, the phenotypic differences between DHs and their initial varieties were examined in respect of yield-related traits. The results indicated that none of the studied varieties was homogenous. The most differentiated varieties appeared to be Kobra and Elena.
Keywords:
genetic similarity, Mahalanobis distance, phenotype, RAPD, wheat varietiesReferences
Caliński T., Kaczmarek Z. 1973. Metody kompleksowej analizy doświadczenia wielocechowego. Trzecie Colloquium Metodologiczne z Agrobiometrii, PAN i PTB Warszawa 258 — 320.
Google Scholar
Chen X. H, Niu Y. C., Hu B. Z. 2004. Identification of RAPD markers linked to the resistance gene Yr5 against wheat stripe rust with denaturing PAGE-silver staining. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Google Scholar
Devos K. M., Gale M. D. 1992. The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet 84:567 — 572.
Google Scholar
Joshi Ch. P., Nguyen H. T. 1993. Application of the random amplified polymorphic DNA techniques for the detection of polymorphism among wild and cultivated tetraploid wheats. Genome 36: 602 — 609.
Google Scholar
Kato K., Miura H., Sawada S. 2000. Mapping QTLs controlling grain yield and its components on chromosome 5A of wheat. Theor. Appl. Genet. 101: 1114 — 1121.
Google Scholar
Kuczyńska A., Bocianowski J., Masojć P., Surma M., Adamski T. 2003. Zastosowanie markerów RAPD do określania podobieństwa genetycznego odmian jęczmienia ozimego (Hordeum vulgare L.). Biul. IHAR 226/227: 81 — 85.
Google Scholar
Laurie D. A., Bennett M. D. 1986. Wheat × maize hybridization. Can. J. Genet. Cytol. 28: 313 — 316.
Google Scholar
Marić S., Bolarić S., Martinčić J., Pejić I., Kozumplik V. 2004. Genetic diversity of hexaploid wheat cultivars estimated by RAPD markers, morphological traits and coefficients of parentage. Plant Breeding 123 (4): 366 — 369.
Google Scholar
Morrison D. F. 1976. Multivariate Statistical Methods. McGraw-Hill; New York.
Google Scholar
Rębarz M., Kuczyńska A., Krystkowiak K., Bocianowski J., Adamski T., Surma M. 2003. Intra- and intervarietal polymorphism in wheat detected by RAPD primers. In: Application of novel cytogenetic and molecular techniques in genetics and breeding of the grasses. PAGEN Series Vol. 1. Eds.: Zwierzykowski Z., Surma M., Kachlicki P. Institute of Plant Genetics PAN, Poznań: 143 — 149.
Google Scholar
Nei M., Li W. H. 1979. Mathematical model for studying genetic variation in terms of restriction end nucleases. Proc. Natl. Acad. Sci. USA. 1979 October; 76 (10): 5269–5273.
Google Scholar
Thomson D. Henry R. 1995 Single-Step Protocol for Preparation of plant tissue for analysis by PCR BioTechniques Vol. 19, No. 3: 394 — 400.
Google Scholar
Wędzony M. 2003. Protocol for doubled haploid production in hexaploid Triticale (Triticosecale Wittm.) by crossing it with maize. In: Doubled haploid production in crop plants. A manual. Eds: M. Maluszynski, K. J. Kasha, B. P. Forster, I. Szalejko. Kluwer Academic Publishers Dordrecht/ Boston/ London: 129 — 134.
Google Scholar
Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research 18 (22): 6531 — 6535.
Google Scholar
Authors
Katarzyna Pankiewiczoffice@igr.poznan.pl
Instytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Tadeusz AdamskiInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Zygm KaczmarekInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Maria SurmaInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Authors
Michał RębarzInstytut Genetyki Roślin Polskiej Akademii Nauk w Poznaniu Poland
Statistics
Abstract views: 29PDF downloads: 29
License
Copyright (c) 2007 Katarzyna Pankiewicz, Tadeusz Adamski, Zygm Kaczmarek, Maria Surma, Michał Rębarz
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Upon submitting the article, the Authors grant the Publisher a non-exclusive and free license to use the article for an indefinite period of time throughout the world in the following fields of use:
- Production and reproduction of copies of the article using a specific technique, including printing and digital technology.
- Placing on the market, lending or renting the original or copies of the article.
- Public performance, exhibition, display, reproduction, broadcasting and re-broadcasting, as well as making the article publicly available in such a way that everyone can access it at a place and time of their choice.
- Including the article in a collective work.
- Uploading an article in electronic form to electronic platforms or otherwise introducing an article in electronic form to the Internet or other network.
- Dissemination of the article in electronic form on the Internet or other network, in collective work as well as independently.
- Making the article available in an electronic version in such a way that everyone can access it at a place and time of their choice, in particular via the Internet.
Authors by sending a request for publication:
- They consent to the publication of the article in the journal,
- They agree to give the publication a DOI (Digital Object Identifier),
- They undertake to comply with the publishing house's code of ethics in accordance with the guidelines of the Committee on Publication Ethics (COPE), (http://ihar.edu.pl/biblioteka_i_wydawnictwa.php),
- They consent to the articles being made available in electronic form under the CC BY-SA 4.0 license, in open access,
- They agree to send article metadata to commercial and non-commercial journal indexing databases.
Most read articles by the same author(s)
- Wojciech Rybiński, Karolina Krystkowiak, Anetta Kuczyńska, Michał Rębarz, Variability of hull-less barley mutants (Hordeum vulgare L.) at the phenotypic and molecular levels , Bulletin of Plant Breeding and Acclimatization Institute: No. 245 (2007): Regular issue
- Karolina Krystkowiak, Tadeusz Adamski, Maria Surma, Zygmunt Kaczmarek, Anetta Kuczyńska, Agata Burtna, Renata Trzeciak, Variability of chosen technological traits of winter wheat hybrids in relation to composition of HMW glutenin subunits in parental forms , Bulletin of Plant Breeding and Acclimatization Institute: No. 260/261 (2011): Regular issue
- Karolina Krystkowiak, Tadeusz Adamski, Maria Surma, Zygmunt Kaczmarek, Anetta Kuczyńska, Variation of wheat cultivars with regard to agronomic traits estimated by uni- and multivariate analysis , Bulletin of Plant Breeding and Acclimatization Institute: No. 253 (2009): Regular issue
- Dorota Jasińska, Anetta Kuczyńska, Krzysztof Mikołajczak, Piotr Ogrodowicz, Hanna Ćwiek-Kupczyńska, Magdalena Anioła, Krzysztof Rubrycki, Róża Mazur, Michał Kempa, Maria Surma, Tadeusz Adamowski, Halina Wiśniewska, Variation of spring barley SSD lines in reaction to infection by fungi of the genus Fusarium , Bulletin of Plant Breeding and Acclimatization Institute: No. 282 (2017): Regular issue
- Tadeusz Adamski, Maria Surma, Aleksandra Ponitka, Aurelia Ślusarkiewicz-Jarzina, Karolina Krystkowiak, Anetta Kuczyńska, Hanna Pudelska, Krzysztof Rubrycki, Renata Trzeciak, Jolanta Woźna, Production efficiency of wheat haploids by anther culture and wheat × maize crosses , Bulletin of Plant Breeding and Acclimatization Institute: No. 252 (2009): Regular issue
- Wojciech Rybiński, Katarzyna Pankiewicz, Jan Bocianowski, Michał Rębarz, Analysis of some traits on phenotypic and molecular level for two- and multi-rowed doubled haploids of spring barley (Hordeum vulgare L.) , Bulletin of Plant Breeding and Acclimatization Institute: No. 249 (2008): Regular issue
- Wojciech Rybiński, Jan Bocianowski, Katarzyna Pankiewicz, Variability of quantitative traits of two mutated grasspea (Lathyrus sativus L.) cultivars , Bulletin of Plant Breeding and Acclimatization Institute: No. 264 (2012): Regular issue
- Tadeusz Adamski, Andrzej Bichoński, Zdzis Biliński, Zbigniew Bystry, Piotr Jarosz, Dorota Jasińska, Zygmunt Kaczmarek, Karolina Krystkowiak, Anetta Kuczyńska, Wojciech Mikulski, Barbara Nowak, Wanda Orłowska-Job, Zdzisław Paszkiewicz, Michał Rębarz, Maria Surma, Anna Sybilska, Identifying suitable locations for evaluating advanced barley breeding lines , Bulletin of Plant Breeding and Acclimatization Institute: No. 247 (2008): Regular issue
- Zygmunt Kaczmarek, Henryk Woś, Elżbieta Adamska, Tadeusz Adamski, Roman Biliński, Grzegorz Budzianowski, Łukasz Mańkowski, Ewelina Majchrzak, Janina Woś, Laurencja Szała, Teresa Cegielska-Taras, Renata Trzeciak, Evaluation of parental lines on the basis of yield of hybrids obtained from incomplete crossing scheme , Bulletin of Plant Breeding and Acclimatization Institute: No. 277 (2015): Regular issue
- Katarzyna Pankiewicz, Wojciech Rybiński, Estimation of genetic variability and of β-ODAP neurotoxin content in chosen species of the genus Lathyrus , Bulletin of Plant Breeding and Acclimatization Institute: No. 250 (2008): Regular issue
