Variability of quantitative traits in grasspea (Lathyrus sativus L.) M1 progeny obtained by seed treatment with chemomutagens
Wojciech Rybiński
office@igr.poznan.plInstytut Genetyki Roślin PAN w Poznaniu (Poland)
Jan Bocianowski
Katedra Metod Matematycznych i Statystycznych, Akademia Rolnicza w Poznaniu (Poland)
Abstract
The aim of the study was to estimate susceptibility of Polish grasspea cultivars Derek and Krab to the effects of two chemomutagens, and to determine the suitable combination of mutagens to be used in grasspea mutagenesis. The grasspea seeds were treated with two chemomutagens: N-nitroso-N-methylurea (MNU) and sodium azide (SA) with either of the mutagens alone and in combinations: SA followed by MNU or MNU followed by SA, using different mutagen doses. For M1 progeny and control (non-treated) plants the plant height, number of lateral branches and yield structure were evaluated. The results of treatment indicate that chemomutagens applied alone or in combinations caused the decrease in value of the analyzed traits except the plant height. A rate of decrease depended on the initial form used, analyzed trait, kind and dose of mutagen, and the combination applied. Plants of cv. Derek, compared to those of cv. Krab, were found to be more susceptible to treatment with mutagens. Somatic injuries induced by MNU were more serious than those caused by SA, and the effects of treatment with the combination SA + MNU were more harmful than those caused by the combination MNU + SA. It may be concluded from the results obtained that to induce a desirable mutation in grasspea, the combination of two reagents acting in a different way in the process of mutagenesis is most effective.
Keywords:
grasspea, mutagens, M1 progeny, somatic injuries, yield structureReferences
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Authors
Jan BocianowskiKatedra Metod Matematycznych i Statystycznych, Akademia Rolnicza w Poznaniu Poland
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