The analysis of genetic diversity of components of rye hybrids
Andrzej Rafalski
a.rafalski@ihar.edu.plZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Małgorzata Gaweł
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Iwona Wiśniewska
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików (Poland)
Abstract
The genetic diversity among 26 maternal single crosses, 8 restorers, 17 male sterile inbred lines and 13 nonrestorer inbred lines was evaluated using PCR marker analysis. These materials were the components of rye hybrids investigated in the preofficial trials at the Plant Breeding and Acclimatization Institute (IHAR), Danko Breeding Co. Ltd (Danko) and Poznań Breeding Co. Ltd (PHR) during the growing seasons 2001–2002. For the evaluation of genetic diversity of rye breeding materials the semi-specific PCR system using primers targeting intron-exon sequences of plant genes was applied. The evaluation of genetic diversity among maternal hybrids and restorers was based on analysis of 317 DNA fragments, and the genetic distances among inbred lines were calculated from analysis of 370 DNA fragments. Both the estimated data of genetic distances of materials and the cluster analysis based on PCR data, indicated that hybrid components from different breeding centres exhibited somewhat different genetic characters.
Keywords:
heterosis, hybrids, inbred lines, rye, semi-specific PCRReferences
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Authors
Andrzej Rafalskia.rafalski@ihar.edu.pl
Zakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Małgorzata GawełZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
Authors
Iwona WiśniewskaZakład Biochemii i Fizjologii Roślin, Instytut Hodowli i Aklimatyzacji Roślin, Radzików Poland
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Copyright (c) 2003 Andrzej Rafalski, Małgorzata Gaweł, Iwona Wiśniewska
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