Optimisation of the conditions for in vitro regeneration of sage
Aleksandra Luwańska
sekretariat@iwnirz.plInstytut Włókien Naturalnych i Roślin Zielarskich, Zakład Biotechnologii, Poznań (Poland)
Karolina Wielgus
Instytut Włókien Naturalnych i Roślin Zielarskich, Zakład Biotechnologii, Poznań (Poland)
Marlena Szalata
Uniwersytet Przyrodniczy, Katedra Biochemii i Biotechnologii, Poznań (Poland)
Daniel Lipiński
Uniwersytet Przyrodniczy, Katedra Biochemii i Biotechnologii, Poznań (Poland)
Joanna Zeyland
Uniwersytet Przyrodniczy, Katedra Biochemii i Biotechnologii, Poznań (Poland)
Ryszard Słomski
Uniwersytet Przyrodniczy, Katedra Biochemii i Biotechnologii, Poznań (Poland)
Abstract
Sage (Salvia officinalis L.) is a useful medicinal plant showing antiseptic activity towards a broad spectrum of bacteria, anti-inflammatory activity, lowering blood sugar, treating stomach ailments, and even having an antioxidant effect. The applicability of a number of chemicals present in the essential oil of sage increases the interest in cultivation this species using biotechnological methods. The development of efficient methods for plant regeneration is important for a medicinal plant, such as sage. It opens up a number of possibilities ranging from unravelling of metabolic pathways and increasing the production of secondary metabolites, the micropropagation of homogeneous genotypes, and finally to genetic transformation and production of biopharmaceuticals. The aim of this study was to develop efficient methods for regeneration of sage in in vitro cultures through direct and indirect organogenesis. Direct organogenesis was induced on Murashige Skoog medium (MS) and the half-strength MS medium (1/2 MS) containing different combinations and concentrations of plant growth regulators: BA, mT, NAA and IAA. The callus induction and plant regeneration trials by indirect organogenesis were conducted in three different experiments, consisting of a set of different media. This experiment included various output explants. As a result of the studies efficient regeneration of sage was developed through direct organogenesis using MS media containing 0.3 mg / l BA and 0.3 mg / l mT. Multiplication rate during cyclic culture was in the range of 3.08 - 3.82 for the media containing BA and 2.44–3.41 for the media with mT. In studies on indirect organogenesis we also obtained induction of callus on all the substrates, while the induction of morphogenetic properties of the tissue requires further development.
Supporting Agencies
Keywords:
induction of callus, micropropagation, in vitro regeneration, plant groeth regulators, sage, multpilication rateReferences
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Authors
Aleksandra Luwańskasekretariat@iwnirz.pl
Instytut Włókien Naturalnych i Roślin Zielarskich, Zakład Biotechnologii, Poznań Poland
Authors
Karolina WielgusInstytut Włókien Naturalnych i Roślin Zielarskich, Zakład Biotechnologii, Poznań Poland
Authors
Marlena SzalataUniwersytet Przyrodniczy, Katedra Biochemii i Biotechnologii, Poznań Poland
Authors
Daniel LipińskiUniwersytet Przyrodniczy, Katedra Biochemii i Biotechnologii, Poznań Poland
Authors
Joanna ZeylandUniwersytet Przyrodniczy, Katedra Biochemii i Biotechnologii, Poznań Poland
Authors
Ryszard SłomskiUniwersytet Przyrodniczy, Katedra Biochemii i Biotechnologii, Poznań Poland
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Copyright (c) 2013 Aleksandra Luwańska, Karolina Wielgus, Marlena Szalata, Daniel Lipiński, Joanna Zeyland, Ryszard Słomski
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