Variability of some ω-gliadins and grain protein content in spelt and cultivar Elena hybrid genotypes
Jacek Waga
j.waga@ihar.edu.plZakład Oceny Jakości i Metod Hodowli Zbóż, Instytut Hodowli i Aklimatyzacji Roślin, Kraków (Poland)
Abstract
Grain protein content is one of the most important factors influencing baking quality of wheat cultivars. Three groups of recombinant lines developed from a cross between spelt and cultivar Elena were investigated in the years 2000, 2001 and 2002 to determine relationships between gliadin electrophoretic spectra and grain protein content. The lines differed in gliadins coded by the chromosome 1B. Two gliadin blocks: Gli B1-6 (spelt type) and Gli B1-1 (Elena type), as well as two individual gliadin bands: S2 (spelt type) i E2 (Elena type) were identified among the analysed genotypes. Analysis of variance proved that protein content of spelt type genotypes (Gli B1-6/S2) was significantly higher than that of Elena type genotypes (Gli B1-1/E2). Recombinant lines that combined spelt and Elena gliadin proteins (Gli B1-6/E2) were characterized by intermediate, but significantly different from parental forms, levels of protein content. The results suggest the existence of the relationships between grain protein content and variability of ω-gliadin subunits. It may be assumed that confirmation of these relationships will make possible to utilize, in the future, the identified gliadin proteins as biochemical markers to reveal the enhanced content of grain protein.
Keywords:
electrophoretic analysis, gliadins, grain protein content, speltReferences
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Authors
Jacek Wagaj.waga@ihar.edu.pl
Zakład Oceny Jakości i Metod Hodowli Zbóż, Instytut Hodowli i Aklimatyzacji Roślin, Kraków Poland
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