Chemical tests for identification and characterization of tomato cultivars

K. Vishwanath

vishwakoti@gmail.com
Department of Seed Science and Technology, University of Agricultural Sciences, GKVK, Bangalore, India (India)

H. M. Pallavi


Department of Seed Technology, University of Horticultural, Sciences Bagalkot, India (India)

N. Nethra


Department of Seed Science and Technology, University of Agricultural Sciences, GKVK, Bangalore, India (India)

S. Rajendra Prasad


Project Directorate (Seeds), Directorate of Seed Research, Mau, India (India)


Abstract

Various chemical tests are being used to reveal chemical differences among the seeds or seedlings of different cultivars. Study to characterize and identify 24 tomato cultivars based on chemical test chemicals viz., Standard phenol test, Modified phenol test, NaOH test, KOH test and Seedling growth response to added chemicals reveled that, most of the cultivars studied were distinct from other cultivars. No single chemical test could distinguish all the varieties. However, distinguishable chemical characteristics were used to develop the keys for identification of each and every cultivar and all the cultivars were distinguished based on these identification

 


Keywords:

chemical tests, tomato, varietal characterization

Abrol Y.P., Uprety D.C. 1972. Phenol colour reaction vis-à-vis discolouration of paddy. Bull. Grain. Technol., 10(4): 288-290
Google Scholar

Agarwal R.L. Karki B.S. 1989. Identification of wheat varieties on the basis of laboratory evaluation. Univ. Agric. Technol. Pantnagar. Res. Bull., 116: 81.
Google Scholar

Agarwal R.L. Pawar A. 1990. Identification of soybean varieties based on seed and seedling characteristics. Seed. Res., 18(1): 77-81.
Google Scholar

Agarwal R.L., Sharma B.L. 1989. Identification of mung bean varieties on the basis of seedling growth response to GA3 and DDT treatment. Seed. Res., 17 (1): 84-87.
Google Scholar

Arus P. Tanksley S.D. Oroton T.J. Jones R.A. 1982. Electrophoresis variation as a tool for determining seed purity and for breeding hybrid varieties of Brassica oleraceae. Euphytica, 31: 417-428.
Google Scholar

Banerjee S.K. Chandra S. 1977. Modified phenol test for the varietal identification of wheat seed. Seed Sci. & Technol., 5(1) 53-60.
Google Scholar

Chauhan J.S. Nanda J.S. 1983. Genetic variability for physico-chemical characters of rice grain in segregation O. sativa L. Oryza 20: 209-215.
Google Scholar

Goyal A.K. Baijal B.D. 1980. Effect of GA and RNase activity and RNA contents at early seedling stage in certain rice (O. sativa L.) genotypes. Indian J. Agric. Res., 14(2): 111-114.
Google Scholar

Gupta P.K. Agarwal R.L. 1988. Determination of varietal purity of paddy varieties by laboratory evaluation. Oryza 25: 310-314.
Google Scholar

ISTA. 1993. Hand book of variety testing: Rapid Chemical identification techniques (Eds. Payme, R.C. USDA). Pub. ISTA, Zurich, Switzerland.
Google Scholar

ISTA. 1996. International Rules for Seed Testing. (Suplliment) Seed Sci. & Technol., 29: 1-135
Google Scholar

Jaiswal J.P. Agarwal R.L. 1995. Varietal purity determination in rice modification of the phenol test. Seed Sci & Technol 23: 33-42.
Google Scholar

Joshi M.G. Banerjee S.K. 1970. Genetics of phenol colour reaction in emmer wheats. Proc. Int. Seed Test. Assoc., 35: 207.
Google Scholar

Lucchese C. Dinelli G. Miggiano A. Lovato. 1999. Identification of pepper (Capsicum spp) cultivars by field and electrophoresis tests. Seed Sci. & Technol., 27:37-47.
Google Scholar

Mc Donald MB Jr. 1985. AOSA cultivar purity subcommittee report. AOSA Newsletter 59(1):40-57.
Google Scholar

Meshram L.D. Rahangdale S.L. 1988. Studies on phenol test in rice. Ann. Pl. Physiol., 2(1): 107-109.
Google Scholar

Nethra N. Rajendra Prasad S. Vishwanath K,. Dhanraj K.N. Ramegowda. 2007. Identification of rice hybrids and their parental lines based on seed, seedling characters, chemical tests and gel electrophoresis of Total soluble proteins. Seed Sci. & Technol., 35:176-186.
Google Scholar

Panse V.S. Sukhatme P.V. 1967. Statistical Methods for Agricultural workers. Indian Council of Agricultural Research Publication. New Delhi, India.
Google Scholar

Panwar R.C, Ram C. 1998. Identification of wheat varieties by phenol and modified phenol tests. Proceedings of National Seminar of Seed Science and Technlogy. March 7-9, 1988, Hissar, India. P.134.
Google Scholar

Papp E. Korosi F. Kemeny A. 1997. Identification of seeds of Brassica species on the basis of spectrophotometry of seed extracts. Seed Sci. & Technol., 25: 577-580.
Google Scholar

Patil. B.N.K, Sangeeta Macha, Motagi B.N. Vijayakumar A.G. Hanchinal R.R. 2006. Charaeterization of safflower varieties through chemical tests. Proceedings of XII National Seed Seminar on prosperity through quality seed, 24-26th February,2006, ANGRAU, Hyderabad, India p. 168
Google Scholar

Prasad H. Roy D. 1999. Development of seed keys for varietal identification in pigeonpea (Cajanus cajan L.) using growth responses to added chemicals. Legume Res., 22(4): 249-253
Google Scholar

Punia R.C. Chandgi R. Tomer R.P.S. Anita M. 2002. Cultivar identification of wheat by modified phenol tests. Seed Tech. News., 32 (1): 97.
Google Scholar

Sparks G.A. May Gibbon D.B. Coles G.D. Slack C.R. 1985. Identification of rice genotypes. Nz. J. Exp. Agric., 13(4): 359-367.
Google Scholar

Sundaru M. Baba I. Tanabe T, Tamai F., Matoda Y. 1983. Varietal differences of Indonesian rice plants in their susceptibility to 2, 4-D injury and inter-relationship with ethylene. Japanese J. Crop Sci., 52(3): 323 -330.
Google Scholar

Takahashi N. Hamaz A.H.A. 1983. Differentiation of ecotypes in Oryza sativa L. I: Re-examination for colour reaction with phenol. Japan J. Breed., 33(3): 243-250.
Google Scholar

Tanksley S.D. Ganal S.D. Prince J.P. Broun P. Roder M.S. Young N.D. 1992. High density molecular maps of the tomato and potato genomes. Genetics, 132: 1141-1460.
Google Scholar

Terao H. 1986. Studies on the mesocotyl elongation of seedlings in Japanese paddy rice cultivars, (O. sativa) L. Bull. Inst. Trop. Agric., 9: 77-87.
Google Scholar

Vanangamudi K. Palanisamy V. Natesan P. 1998. Variety determination in rice- phenol and potassium hydroxide tests. Seed Sci. & Technol., 16: 465-470.
Google Scholar

Vishwanath K. Ananthararayanan T.V. Pallavi H.M. Ramegowda Rajendra Prasad S. Prasanna K.P.R. Shailaja H. 2010. Varietal Characterization of Tomato Cultivars Based on RAPD Markers. Res. J. Agril. Biol. Sci., 6(6): 713-715.
Google Scholar

Walls F.W. 1965. Standardization of phenol method for testing of wheat for varital purity. Hand Book on Seed Testing, AOSA, Contribution No. 28.
Google Scholar

Wang J. Shen Z. 1992. Study on the classification of American rice varieties. Crop Gen. Resour., 2: 4-6.
Google Scholar

Wang X.F. Konoblauch R. Leist N. 2000. Varietial discrimination of tomato (Lycopersicon esculentum Mill.) by ultra thin – layer isoerlectric focusing of seed protein, Seed Sci & Technol., 28:521-526.
Google Scholar

Download


Published
2013-12-19

Cited by

Vishwanath, K., Pallavi, H. M., Nethra, N., & Rajendra Prasad, S. (2013). Chemical tests for identification and characterization of tomato cultivars. Plant Breeding and Seed Science, 68, 3–13. Retrieved from http://ojs.ihar.edu.pl/index.php/pbss/article/view/289

Authors

K. Vishwanath 
vishwakoti@gmail.com
Department of Seed Science and Technology, University of Agricultural Sciences, GKVK, Bangalore, India India

Authors

H. M. Pallavi 

Department of Seed Technology, University of Horticultural, Sciences Bagalkot, India India

Authors

N. Nethra 

Department of Seed Science and Technology, University of Agricultural Sciences, GKVK, Bangalore, India India

Authors

S. Rajendra Prasad 

Project Directorate (Seeds), Directorate of Seed Research, Mau, India India

Statistics

Abstract views: 135
PDF downloads: 0


License

All articles published in electronic form under CC BY-SA 4.0, in open access, the full content of the licence is available at: https://creativecommons.org/licenses/by-sa/4.0/legalcode.pl .