Resistance of Phytophthora infestans in three Solanum nigrum F3 families
Abstract
Solanum nigrum is a self-pollinating, hexaploid weed and one of a few Solanaceae species native to Europe. It used to be described as a non-host for Phytophthora infestans. However, now it is known that, like its distant relatives: potato (Solanum tuberosum L.) and tomato (Solanum lycopersicum L.), S. nigrum can suffer from potato late blight caused by this pathogen. Both susceptible and resistant S. nigrum genotypes have been previously identified and inheritance of resistance originating from one accession has been described based on population of F2 plants and 15 F3 lines. The goal of this study was to evaluate resistance of three families of F3 lines, originating from crosses between a susceptible and three different resistant S. nigrum accessions followed by two self-pollinations. Parental acces- sions were tested for the spectrum of late blight resistance against 48 P. infestans isolates. The three families consisted of 106, 96 and 115 F3 lines, respectively, and from each line 20 plants were tested for resistance to P. infestans. Laboratory detached leaf assays were performed in two dates and two replications of three leaves each. Segregation of the trait within the line allowed us to distinguish hetero- and homozygous lines. In one F3 family, the ratio of resistant homozygotes: heterozygotes: susceptible homozygotes was 1:2:1, indicating that a single gene is most likely underlying the late blight resistance in this case. In the other two, observed segregations of the trait significantly deviated from this model suggesting more complex inheritance patterns.
Keywords
black nightshade; inheritance; late blight; potato; Solanum tuberosum; virulence
References
Colon L. T., Eijlander R., Budding D. J., Pieters M. M. J., Hoogendoorn J., Van-Ijzendoorn M. T. 1993. Resistance to potato late blight (Phytophthora infestans (Mont.) de Bary) in Solanum nigrum, S. villosum and their sexual hybrids with S. tuberosum and S. demissum. Euphytica, 66: 55–64.
Deahl K. L., Shaw D. S., Cooke L. R. 2004. Natural occurrence of Phytophthora infestans on black nightshade (Solanum nigrum) in Wales. Plant Disease, 88: 771.
Eijlander R., Stiekema W. J. 1994. Biological containment of potato (Solanum tuberosum): outcrossing to the related wild species black nightshade (Solanum nigrum) and bittersweet (Solanum dulcamara). Sex. Plant. Reprod., 7:29–40.
Flier W. G., van der Bosch G.M. B, Turkensteen L. J. 2003. Epidemiological importance of Solanum sisymbriifolium, S. nigrum and S. dulcamara as alternative hosts for Phytophthora infestans. Plant Pathol., 52: 595–603.
Golas T. M., Sikkema A., Gros J., Feron R. M., van den Berg R. G., van der Weerden G. M., Mariani C., Allefs J. J. 2010. Identification of a resistance gene Rpi-dlc1 to Phytophthora infestans in European accessions of Solanum dulcamara. Theor. Appl. Genet., 120: 797-808.
Golas T. M., van de Geest H., Gros J., Sikkema A., D'Agostino N., Nap J. P., Mariani C., Allefs J. J., Rieu I. 2012. Comparative next-generation mapping of the Phytophthora infestans resistance gene Rpi-dlc2 in a European accession of Solanum dulcamara. Theor. Appl. Genet., 126: 59-68.
Haverkort A. J., Boonekamp P. M., Hutten R., Jacobsen E., Lotz L. A. P., Kessel G. J. T., Visser R. G. F., van der Vossen E. A. G. 2008. Societal cost of late blight in potato and prospects of durable resistance through cisgenic modification. Potato Res., 51: 47-57.
Horsman K., Bergervoet J. E. M., Jacobsen E. 1997. Somatic hybridization between Solanum tuberosum and species of the S. nigrum complex: Selection of vigorously growing and flowering plants. Euphytica, 96: 345–352.
Lebecka R. 2008. Host-pathogen interaction between Phytophthora infestans and Solanum nigrum, S. villosum, and S. scabrum. Eur. J. Plant. Pathol., 120: 233-240.
Lebecka R. 2009. Inheritance of resistance in Solanum nigrum to Phytophthora infestans. Eur. J. Plant. Pathol. 124: 345-348.
Lilliefors H. 1967. On the Kolmogorov–Smirnov test for normality with mean and variance unknown. J. Am. Statistical Assoc., 62: 399–402.
Malcolmson J. F. 1969. Races of Phytophthora infestans occurring in Great Britain. Trans. Brit. Mycol. Soc., 53: 417-423.
Platt H. W. 1999. Response of solanaceous cultivated plants and weed species to inoculation with A1 or A2 mating type strains of Phytophthora infestans. Can. J. of Plant Pathol., 21: 301–307.
Śliwka J., Jakuczun H., Lebecka R., Marczewski W., Gebhardt C., Zimnoch-Guzowska E. 2006. The novel, major locus Rpi-phu1 for late blight resistance maps to potato chromosome IX and is not correlated with long vegetation period. Theor. Appl. Genet., 113: 685-695.
Smirnov N.V. 1944. Approximate distribution laws for random variables, constructed from empirical data. Uspekhi Mat. Nauk, 10: 179–206 (In Russian).
Świeżyński K. M., Zimnoch-Guzowska E. 2001. Breeding potato cultivars with tubers resistant to Phytophthora infestans. Potato Res., 44: 97-117.
Szczerbakowa A., Maciejewska U., Zimnoch-Guzowska E., Wielgat B. 2003. Somatic hybrids Solanum nigrum (+) S. tuberosum: morphological assessment and verification of hybridity. Plant Cell Rep., 21: 577–584.
Zimnoch-Guzowska E., Lebecka R., Kryszczuk A., Maciejewska U., Szczerbakowa A., Wielgat B. 2003. Resistance to Phytophthora infestans in somatic hybrids of Solanum nigrum L. and diploid potato. Theor. Appl. Genet., 107: 43–48.
Plant Breeding and Acclimatization Institute-National Research Institute, Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland Poland
Plant Breeding and Acclimatization Institute-National Research Institute, Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland Poland
Plant Breeding and Acclimatization Institute-National Research Institute, Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland Poland
Plant Breeding and Acclimatization Institute-National Research Institute, Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland Poland
Plant Breeding and Acclimatization Institute-National Research Institute, Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland Poland
Plant Breeding and Acclimatization Institute-National Research Institute, Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland Poland
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