Genetic variability of sotol (Dasyrylion cedrosanum) populations in the Mexican Coahuila southern area
Norma Paola Meléndez Rentería
Food Research Department. School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza s/n, Col. Republica oriente. CP. 25280. Saltillo, Coahuila, México (Mexico)
Veronica Padilla
2Biotechnology Department. School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza s/n, Col. Republica oriente. CP. 25280. Saltillo, Coahuila, México (Mexico)
Gerardo Gaona Lozano
Biotechnology Department. School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza s/n, Col. Republica oriente. CP. 25280. Saltillo, Coahuila, México (Mexico)
Cristobal Noe Aguilar
Food Research Department. School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza s/n, Col. Republica oriente. CP. 25280. Saltillo, Coahuila, México. (Mexico)
Raúl Rodríguez-Herrera
raul.rodriguez@uadec.edu.mxFood Research Department. School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza s/n, Col. Republica oriente. CP. 25280. Saltillo, Coahuila, México. (Mexico)
Abstract
In the Chihuahuan desert area, in Mexico, exist an endemic plant denominated Sotol (Dasylirion spp); which is used by rural producers to manufacture a liqueur named Sotol. This beverage has origin denomination but it industrial application has lack scientific reports, as well as the plant genetic information. This is the first study about ecological distribution of sotol plants and, may be, a first step to continue the scientific investigation of a Mexican endemic plant, with many potential industrial applications. In this paper, we used AFLP molecular markers to calculate population genetics parameters as genetic diversity, a conglomerate analyses, poblational structure based in Wright´s statistics and analysis of molecular variance. Differences in soil composition and weather conditions, of the sampling region, may affect sotol plants growth and their morphologic characteristics; as consequence the organisms generate ample genetic variability, which ensures the species permanence. Genetic diversity values were similar to the average for cross-pollinated plants, while the heterozygosis detection was deficient; Wright statistics suggest plants genetics interchange in the subpopulation; AMOVA found significant differences within and between collections. These results help us to maintenance the genetic diversity, in order to prevent potential extinction and guarantee sustainable development; the preservation of the endemic plants is necessary to the ecosystems conservation and after Sotol origin denomina- tion, by the Mexican government, the importance of D. cedrosanum plants was increased.
Keywords:
AFLP, Genetic Diversity, Sotol, Dasylirion cedrosanumReferences
Alfaro-Rojas G, Legaria-Solano JP, Rodriguez-Perez JE (2007) Genetic diversity in populations of pulquero agaves (Agave spp.) in northeastern Mexico state. Fitotecnica Mexicana 30(1):1-12
Google Scholar
Arce-González L, Valdés-Reyna J, Valdés-Oyervides A, Gallegos-Del Trejo A, Calderón-Gómez ER (2003) Latecy break in sotol (Dasylirion cedrosanum Trel) seeds by means of physical scarification and sulfuric acid. In: Investigation proyect results. Direccion de investigacion. Universidad Autonoma Agraria Antonio Narro. Saltillo, Coahuila, Mexico, pp. 496-500
Google Scholar
Balzarini M, Di-Rienzo J (2004) Info-Gen: Software for statistical analysis of genetic data Facultad de Ciencias Agropecuarias. Universidad Nacional de Córdoba, Argentina
Google Scholar
Balzarini M, Bruno C, Peña A, Teich I, Rienzo J (2010) Estadistica en biotecnología. Aplicaciones en Info- Gen. Encuentro grupo editor. Cordoba, Argentina
Google Scholar
Barraza-Morales A, Sanchez-Teyer L, Robert M, Esqueda M, Gardea A (2006) Genetic variability in Agave angustifolia Haw at the Sonoran sierra, Mexico, determined by AFLP markers. Rev Fitotec Mex 29(1):1- 8
Google Scholar
Bellon MR, Barrientos-Prego AF, Colunga-GarciaMarin P, Perales H, Reyes-Agüero JA, Rosales-Serna R, Zizumbo-Villarreal D (2009) Diversity and conservation of genetic recourses in cultivated plants. In: CONABIO (ed) Natural capital from Mexico, Vol II: Conservation status and change trends, Mexico, pp 355-382
Google Scholar
Bogler D (1998) Three new species of Dasylirion (Nolinaceae) from Mexico and a clarification of the D. longissimum complex. Brittonia 50(1):71-86 doi: 10.2307/2807720
Google Scholar
Brugmans B, Gm Van Der Hulst R, Visser GF, Lindhout R, Van Eck PHJ (2003) A new and versatile method for the successful conversion of AFLP markers into simple single locus markers. Nucleic Acids Res 31 (10):1-9 doi:10.1093/nar/gng055
Google Scholar
Cruz-Requena M (2007) Physicochemical characteristics of male and female sotol plants. Dissertation, Universidad Autonoma de Coahuila
Google Scholar
Cruz-Requena M, Rodríguez-Herrera R, De la Garza TH, Aguilar CN, Contreras JC (2007) Sotol: cuando el sexo y el alcohol se combinan. Ciencia Acierta 11(3):40-41
Google Scholar
De la Garza-Toledo H, Martínez-Sosa M, Lara-Fernández L, Cruz-Requena M. Saucedo-Pompa, S., Rodríguez-Herrera, R., Aguilar-González, C. (ed) (2008a) Sotol, Dasylirion spp. In: Outstanding phyto- chemicals from Mexican semi-desert: From plant to natural chemicals and biotechnology, Path-Design, Mexico.
Google Scholar
De la Garza-Toledo H, Martínez M, Lara L, Rodríguez-Herrera R, Rodriguez-Martinez J, Aguilar CN (2008b) Production of a mexican alcoholic beverage: Sotol. Res J Biological Sciences 3(6):566-571
Google Scholar
De la Garza-Toledo H, Buenrostro J, Reyes-Vega M, Rodríguez-Herrera R, Martínez DG, Aguilar CN (2010) Chemical profile of sotol analyzed by solid phase microextraction-gas chromatography. Am J Agric Biological Sciences 5(3):261-268
Google Scholar
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation version II. Plan molecular biolo reporter. doi:10.1007/BF02712670
Google Scholar
Demey JR, Gamez E, Molina S, Infante D (2004) Comparative study of the discriminating capacity of AFLP and ISTR markers for genetic analysis of Agave fourcroydes. Plant Molec Biol Rep 22:29-35. doi:10.1007/BF02773346
Google Scholar
Gil-Vega K, Diaz C, Nava-Cedillo A, Simpson J (2006) AFLP analysis of Agave tequilana varieties. Plant Sci 170:904-909. doi:10.1016/j.plantsci.2005.12.014.
Google Scholar
Gottileb LD (1981) Electrophoretic evidence and plant populations. Progress in phytochem 7:1-6.
Google Scholar
Hagen IS, Khadari B, Lambert P, Audergon JM (2002) Genetic diversity in apricot revealed by AFLP mark- ers: species and cultivar comparisons. Theor Appl Genet 105:298-305. doi: 10.1007/s00122-001-0910-8
Google Scholar
Ibiza V, Blanca J, Cañizares J, Nuez F (2011) Taxonomy and genetic diversity of domesticated Capsicum species in the Andean region. Genet Resour Crop Evol. doi: 10.007/s10722-011-9744-z
Google Scholar
INEGI (2012) Consulta de la geografia del estado de Coahuila. http://www.inegi.org.mx/default.aspx. Accessed 17 January 2012
Google Scholar
Joy N, Abraham Z, Soniya EV (2007) A preliminary assessment of genetic relationships among agronomically important cultivars of black pepper. BMC Genetics, 8:42 doi: 10.1186/1471-2156/8/42
Google Scholar
Liu J, Wang L, Geng Y, Wang Q, Luo L, Zhong Y (2006) Genetic diversity and population structure of Lamiophlomis rotate (Lamiaceae), an endemic species of Qinghai-Tibet plateau. Genetica 128:385-394 doi:10.1007/s10709-006-7517-y
Google Scholar
Lopez-Alonso D, Rosales-Romero I, Jimenez-Viola A, Pascual-Reguera L (1988) Genetic variablility and evolutive relations among caper bush varieties. Boletín del Instituto de Estudios Almerienses. Ciencias 8:85-115
Google Scholar
Martinez MC, Helguera M, Carrera A (2010) Marcadores moleculares. In: Levitus G, Echenique V, Rubinstein C, Hopp E, Mroginski L (ed) Biotecnologia y mejoramiento Vegetal II. ArgenBio-INTA, Argentina, pp. 70-85
Google Scholar
Meléndez-Rentería NP, Silva-Vázquez R, Nevárez-Moorillón GV, Aguilar CN, Rodríguez-Herrera R (2010) Genetic diversity of Mexican oregano Lippia berlandieri Schauer from the Chihuahuan desert area. Plant Breeding and Seed Science 62:85-96 doi: 10.2478/v10129-011-0007-0
Google Scholar
NOM-159-SCFI-2004 (2004) Norma Oficial Mexicana, bebidas alcoholicas-sotol-especificaciones y métodos de prueba. http://www.ordenjuridico.gob.mx/Federal/PE/APF/APC/SE/Normas/Oficiales/NOM-159- SCFI-2004.pdf, accessed 18 January 2012
Google Scholar
Ocampo-Velázquez R, Malda-Barrera GX, Suarez-Ramos G (2009) Reproductive biology of Mexican oregano (Lippia graveolens Kunth) in three exploitation conditions. Agrociencia. 43:475-482
Google Scholar
Palma EJ (2000) Bases para la propagación de sotol (Dasylirion spp) vías in vitro y por semilla. Dissertation, Universidad Autónoma de Chihuahua
Google Scholar
Piñero D (2008) Genetic diversity as instrument to biodiversity conservation and exploitation: Mexican species studies. In: CONABIO (ed) Natural capital from Mexico, Vol I: Biodiversity actual knowledge, Mexico, pp 437-494
Google Scholar
Rodriguez-Herrera R, Reyes-Valdes MH. Saucedo-Pompa S, Rodriguez-Herrera R, Aguilar-Gonzalez C (ed) (2008) Genetic Diversity study in plants using DNA based molecular markers. In: Outstanding phytochemicals from mexican semi-desert: From plant to natural chemicals and biotechnology, Path-Design,Mexico.
Google Scholar
Sanchez-Teyer F, Moreno-Salazar S, Esqueda M, Barraza A, Robert ML (2009) Genetic variability of wild Agave angustifolia populations based on AFLP: A basic study for conservation. J Arid Env 73(6-7):611- 616 doi: 10.1016/j.jaridenv.2009.01.008
Google Scholar
SEMARNAT. 2012. Consulta de condiciones climaticas del estado de Coahuila, http://smn.cna.gob.mx/. Accessed 17 January 2012
Google Scholar
Vos P, Hogers R, Bleeker M, Reijans M, Van De Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23(21):4407-4414
Google Scholar
Zarate LA (2003) Poblaciones y su condición: Estudio regional del sotol. Universidad Autónoma Agraria “Antonio Narro”. Secretaría de Fomento Agropecuario, Gobierno del Estado de Coahuila. pp 11-14
Google Scholar
Authors
Norma Paola Meléndez RenteríaFood Research Department. School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza s/n, Col. Republica oriente. CP. 25280. Saltillo, Coahuila, México Mexico
Authors
Veronica Padilla2Biotechnology Department. School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza s/n, Col. Republica oriente. CP. 25280. Saltillo, Coahuila, México Mexico
Authors
Gerardo Gaona LozanoBiotechnology Department. School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza s/n, Col. Republica oriente. CP. 25280. Saltillo, Coahuila, México Mexico
Authors
Cristobal Noe AguilarFood Research Department. School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza s/n, Col. Republica oriente. CP. 25280. Saltillo, Coahuila, México. Mexico
Authors
Raúl Rodríguez-Herreraraul.rodriguez@uadec.edu.mx
Food Research Department. School of Chemistry, Universidad Autónoma de Coahuila. Blvd. V. Carranza s/n, Col. Republica oriente. CP. 25280. Saltillo, Coahuila, México. Mexico
Statistics
Abstract views: 162PDF downloads: 67
License
All articles published in electronic form under CC BY-SA 4.0, in open access, the full content of the licence is available at: https://creativecommons.org/licenses/by-sa/4.0/legalcode.pl .
