Evaluation of oat germplasm for resistance to Fusarium Head Blight.
T. Gagkaeva
Laboratory of Mycology and Phytopathology, All-Russian Institute of Plant Protection (VIZR), St. Petersburg, Russia. (Russian Federation)
O. Gavrilova
Laboratory of Mycology and Phytopathology, All-Russian Institute of Plant Protection (VIZR), St. Petersburg, Russia. (Russian Federation)
T. Yli-Mattila
Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland. (Finland)
I. Loskutov
Department of Genetic Resources of rye, barley and oat, N.I.Vavilov All-Russian Research Institute of Plant Industry, St. Petersburg, Russia (Russian Federation)
Abstract
The objectives of this study were to screen the VIR Avena germplasm collection for Fusarium head blight (FHB) resistance and to identify the resistant oat genotypes by using the different scoring of the disease. After artificial inoculation harvested grain samples were assays on the combination of three parameters: percentage of Fusarium damaged kernels (FDK), DNA of trichothecene-producing Fusarium fungi and mycotoxin accumulation. The clear correlation between the parameters for every individual genotype was not detected. The results support the several components of resistance to Fusarium head blight in oats (invasion, spreading and mycotoxin accumulation), which are controlled by different genetic systems. The hull-less genotypes consid- ered to be more resistant in the Avena germplasm. Seven landraces genotypes and five cultivars originated from Asian region and two cultivars originated from European region seem to be suitable genetic resources for resistance to FHB.
Keywords:
Avena, disease, Fusarium, germplasm, kernel, method, resistanceReferences
Batalova G.A. 2000. The oats. The technology of cultivation and breeding. Kirov: The research agriculture. 126–128 (in Russian).
Google Scholar
Boutigni A-L., Richard-Forget F., Barreau C. 2008. Natural mechanisms for cereal resistance to the accumulation of Fusarium trichothecenes. Eur J. Plant Pathol. 121: 411–423.
Google Scholar
European Commission. 2005. Community Reference Laboratory for GM Food and Feed. Event-specific for the quantitation of maize line NK603 using real-time PCR.
Google Scholar
FAOSTAT. 2008. http://faostat.fao.org/
Google Scholar
Gagkaeva T.Yu., Gavrilova O.P. 2009. Fusarium head blight — actual problem of obtaining of qualitative cereal crops. Plant Protection and Quarantine. 12: 13–15 (in Russian).
Google Scholar
Gagkaeva T.Yu., Levitin M.M., Nazarova L.N., Sanin S.S. 2009. Infected grain and complex of Fusarium fungi on the territory of Russia Federation for 2004-2006. Agro XXI. 4/6: 4–6 (in Russian).
Google Scholar
Gavrilova O.P., Gagkaeva T. Yu. 2010. Fusarium head blight of small grain cereals harvested in the north of Nechernozem region and in Kaliningrad region in 2007-2008. Plant Protection and Quarantine. 2: 23–25 (in Russian).
Google Scholar
Gavrilova O.P., Gagkaeva T.Yu., Burkin A.A., Kononenko G.P. 2009. Mycological infection by Fusarium strains and mycotoxins contamination of oats and barley grain in the north of Nonchernozem’e. Agrobiology. 6: 89–93 (in Russian).
Google Scholar
Halstensen A.S., Nordby K.C., Eduard W., Klemsdal S.S. 2006. Real-time PCR detection of toxigenic Fusarium in airborne and settled grain dust and associations with trichothecene mycotoxins. J. Environm. Monitoring. 8: 1235–1241.
Google Scholar
Hilton A.J., Jenkinson P., Hollins T.W., Parry D.W. 1999.Relationship between cultivar height and severity of Fusarium ear blight in wheat. Plant Pathol. 48: 202–208.
Google Scholar
Ivaschenko, V.G., Shipilova, N.P., Nefedova L.I., Gagkaeva T.Ty., Nazarovskay L.A., Chlopunova L.B. 1997. Biology and sanitary surveillance of Fusarium head blight. Mycology and Phytopathology. 31/2: 58–63 (in Russian).
Google Scholar
Jestoi M., Rokka M., Yli-Mattila T., Parikka P., Rizzo A. , Peltonen K. 2004. The presence and concentrations of the Fusarium-related mycotoxins Beauvericin, Enniatins and Moniliformin in Finnish grain samples. Food Additives and Contaminants. 21: 794–802.
Google Scholar
Kononenko G.P., Burkin A.A. 2002. Fusariotoxins in grain feedstuff. Veterinary pathology. 2: 128–132 (in Russian).
Google Scholar
Kononenko G.P., Burkin A.A., Soboleva N.A., Zotova E.V. 1999. Enzyme immunoassay of T-2 toxin in contaminated grain. Applied biochemistry and microbiology. 35/4: 457–462 (in Russian).
Google Scholar
Langseth W., Bernhoft A., Rundberget T., Kosiak B. and Gareis M. 1999. Mycotoxin production and cytotoxicity of Fusarium strains isolated from Norwegian cereals. Mycopathologia. 144: 103–113.
Google Scholar
Legzdina L., Buerstmayr H. 2004. Comparison of infection with Fusarium head blight and accumulation of mycotoxins in grain of hull-less and covered barley. J. Cereal Sci. 40: 61–67.
Google Scholar
Lienemann K., Oerke E-C., Dehne H-W. 2000. Effect of differences amoung wheat genotypes on the occurrence and damage caused by Fusarium head blight. In Proceedings of the 6th European Fusarium seminar, Berlin, Germany. 26.
Google Scholar
Loskutov I.G. 2007. The oats (Avena L.) — distribution, classification, evolution and breeding value. SanktPetersburg: VIR. (in Russian).
Google Scholar
Mesterhasy A. 1995. Types and components resistance to Fusarium head blight of wheat. Plant Breed. 114: 377–386.
Google Scholar
Mesterházy Á. 2002. Theory and practice of the breeding for Fusarium head blight in wheat. J. Appl. Genetics, 43A: 289–302.
Google Scholar
Mielniczuk E., Kiecana I., Perkowski J. 2004. Susceptibility of oat genotypes to Fusarium crookwellense Burgess, Nelson and Toussoun infection and mycotoxin accumulation in kernels. Biologia. 59/6: 809– 816.
Google Scholar
Parikka P., Hietaniemi V., Rämö S., Jalli H. 2008. Fusarium infection and mycotoxins contents of oats under different tillage treatments. 8th International oat Conference. Minneapolis, USA. Session V – Effective Pest.
Google Scholar
Parry D. W., Jenkins P., McLeod L. 1995. Fusarium ear blight (scab) in small grain cereals – a review. Plant Pathol. 44: 207–238.
Google Scholar
Redaelli R., Sgrulletta D., Scalfati G., De Stefanis E., Cacciatori P. 2009. Naked Oats for Improving Human Nutrition: Genetic and Agronomic Variability of Grain Bioactive Components. Crop Sci. 49: 1431– 1437.
Google Scholar
Sarlin T., Yli-Mattila T., Jestoi M., Rizzo A., Paavanen-Huhtala S., Haikara A. 2006. Real-time PCR for quantification of toxigenic Fusarium species in barley and malt. European Journal of Plant Pathology, 114: 371–380.
Google Scholar
Scudamore K.A., Baillie H., Patel S., Edwards S.G. 2007. The occurrence and fate of Fusarium mycotoxins during the industrial processing of oats in the UK. Food Additives and Contaminants. 24/12: 1374-1385.
Google Scholar
Shipilova N.P., Gagkaeva T.Yu. 1992. Fusarium head blight in north-western region of Russia. Plant Protection. 11: 7–8 (in Russian).
Google Scholar
Šlikova S., Šrobarova A., Šudyova V., Polišenska I., Gregova E., and Mihalik D. 2010. Response of oat cultivars to Fusarium infection with a view to their suitability for food use. Biologia. 65/4: 609–614
Google Scholar
Snijders C.H.A. 1990. The inheritance of resistance to head blight caused by Fusarium culmorum in winter wheat. Euphitica. 50: 9–17.
Google Scholar
Stenglein S.A. 2009. Fusarium poae: a pathogen that needs more attention. J. of Plant Pathol. 91: 25–36.
Google Scholar
Tekauz A., B. McCallum N. Ames., and Mitchell Fetch J.W. 2004. Fusarium head blight of oat: Current status in western Canada. Can. J. Plant Pathol. 26:473–479
Google Scholar
Tekauz A., Fetch M. J., Rossnagel B.G., Savard M.E. 2008. Progress in assessing the impacy of Fusarium head blight on oat in western Canada and screening of Avena germplasm for resistance. Cereal Res. Comm. 36: 49–56.
Google Scholar
Uhlig S., Jestoi M. and Parikka P. 2007. Fusarium avenaceum – the North European situation. Int. J. Food Microbiol. 119: 17–24.
Google Scholar
Yli-Mattila T., Paavanen-Huhtala S., Parikka P., Hietaniemi V., Jestoi M., Gagkaeva T., Sarlin T., Haikara A., Laaksonen S., Rizzo A. 2008. Real-time PCR detection and quantification of Fusarium poae, F. graminearum, F. sporotrichioides and F. langsethiae as compared to mycotoxin production in grains in Finland and Russia. Archives of Phytopathol. and Plant Protection. 41: 243–260.
Google Scholar
Yli-Mattila T., Parikka P, Lahtinen T., Ramo S., Kokkonen M., Rizzo A., Jestoi M., Hietaniemi V. 2009. Fusarium DNA levels in Finnish cereal grains In: Current Advances in Molecular Mycology. Gherbawy Y., Mach L., Rai M. (Eds.). Nova Science Publishers. Inc., New York: 107–138
Google Scholar
Yli-Mattila, T. 2010. Ecology and evolution of toxigenic Fusarium species in cereals in northern Europe and Asia. J. Plant Pathol. 92: 7–18.
Google Scholar
Zinkernagel V., Adolf B., Habermeyer J., 1997. The spread of Fusarium spp. from the above ground level to the ears of wheat. Cereal Res. Comm. 25: 677–679.
Google Scholar
Authors
T. GagkaevaLaboratory of Mycology and Phytopathology, All-Russian Institute of Plant Protection (VIZR), St. Petersburg, Russia. Russian Federation
Authors
O. GavrilovaLaboratory of Mycology and Phytopathology, All-Russian Institute of Plant Protection (VIZR), St. Petersburg, Russia. Russian Federation
Authors
T. Yli-MattilaMolecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014 Turku, Finland. Finland
Authors
I. LoskutovDepartment of Genetic Resources of rye, barley and oat, N.I.Vavilov All-Russian Research Institute of Plant Industry, St. Petersburg, Russia Russian Federation
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