Map kinase MGV1: a potential shared control point of butenolide and deoxynivalenol biosynthesis.
Christof Rampitsch
Cereal Research Centre, Agriculture and Agri-Food Canada, Winnipeg MB, Canada. (Canada)
Winnie Leung
Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa ON, Canada. (Canada)
Barbara Blackwell
Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa ON, Canada. (Canada)
Rajagopal Subramaniam
subramaniamra@agr.gc.caEastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa ON, Canada. (Canada)
Abstract
The mitogen-activated protein kinase (MAPK) MGV1 has been knocked out in Fusarium graminearum to produce the mutant Fg∆MGV1. The mutant displays complementary phenotypes concerning deoxynivalenol (DON) and butenolide (BT) biosynthesis in vitro. In the rich medium 15-ADON accumulates are at low levels as detected by HPLC, whereas the accumulation of BT is substantial in Fg∆MGV1. This is supported by the high expression of butenolide cluster genes in the mutant compared to the wild-type strain. Under nutrient-limiting conditions, where DON biosynthesis is normally favoured, the expression of genes of the trichothecene cluster does not differ between the two strains. However, the accumulation of 15-ADON is vastly different in Fg∆MGV1. There is a reduction of 15-ADON accumulation with a concomitant accumula- tion of a novel compound. Although gene clusters comprising the synthesis of DON and BT have been identi- fied, their regulation at the molecular level has not been fully elucidated. Since the expression levels of two regulatory genes from the trichothecene gene cluster and three regulatory genes from the butenolide gene cluster remained unchanged between WT and Fg∆MGV1, we suggest that differential accumulation of both BT and DON biosynthesis is at least partially under post-transcriptional and/or post-translational control.
Keywords:
Gibberella zeae, scabReferences
Argyris J., TeKrony D., Hershman D., VanSanford D., Hall M., Kennedy B., Rucker M, Edge C., 2005. Fusarium head blight infection following point inoculation in the greenhouse compared with movement of Fusarium graminearum in seed and floral components. Crop Sci. 45: 626–634.
Google Scholar
Brown D., Dyer R., McCormick S, Kendra D., Plattner R. 2004. Functional demarcation for the Fusarium core tricothecen gene cluster. Fungal Genet. Biol. 41: 454-462.
Google Scholar
Desjardins A., Hohn T., McCormick S. 1993. Tricothecene biosynthesis in Fusarium species: chemistry, genetics and significance. Microbiol. Rev. 57: 595-604.
Google Scholar
Dyer R., Plattner R., Kendra D., Brown D. J. 2005. Fusarium graminearum TRI14 is required for high virulence and DON production on wheat but not for DON synthesis in vitro. Agric. Food Chem. 53: 9281- 9287.
Google Scholar
Harris L. J., Alexander N. J., Saparno A., Blackwell B., McCormick S.P., Desjardins A.E., Robert L.S., Tinker N., Hattori J., Piché C., Schernthaner J.P., Watson R., Ouellet T. 2007. A novel gene cluster in Fusarium graminearum contains a gene that contributes to butenolide synthesis. Fungal Genet. Biol. 44: 293–306.
Google Scholar
Hou Z., Xue C., Peng Y., Katan T., Kistler C., Xu J. 2002. A mitogen-activated protein kinase gene (MGV1) in Fusarium graminearum is required for female fertility, heterokaryon formation and plant infection. Molec. Plant Micr. Interact. 15: 1119-1127.
Google Scholar
Miller J.D., Blackwell B.A. 1986. Biosynthesis of 3-acetyldeoxynivalenol and other metabolites by Fusarium culmorum HLK 1503 in a stirred jar fermentor. Can. J. Bot. 64: 1–5.
Google Scholar
Placinta C.M., D’Mello J.P.F., Macdonald A.M.C. 1999. A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Anim. Feed Sci. Tech. 78: 21–37.
Google Scholar
Proctor R., Hohn T., McCormick S., Desjardins A. 1995. Tri6 encodes an unusual zinc-finger protein involved in regulation of tricothecene biosynthesis in Fusarium sporotrichoides. Appl. Env. Microbiol. 61: 1923-1930.
Google Scholar
Proctor R., Hohn T., McCormick S. 1995b. Reduced virulence of Giberella zeae caused by disruption of a tricothecene toxin biosynthetic gene. Molec. Plant Micr. Interact. 4: 593-601.
Google Scholar
Rampitsch C., Subramaniam R., Djuric-Ciganovic S., Bykova N.V. 2010. The phosphoproteome of Fusarium graminearum at the onset of nitrogen starvation. Proteomics 10: 124-140.
Google Scholar
Trail F, 2009. For Blighted Waves of Grain: Fusarium graminearum in the Postgenomics Era. Plant Physiol. 149: 103-110.
Google Scholar
Yates S.G., Tookey H.L., Ellis J.J., Tallent W.H., Wolff I.A. 1969. Mycotoxins as a possible cause of fescue toxicity. J. Agric. Food Chem. 17: 437–442.
Google Scholar
Authors
Christof RampitschCereal Research Centre, Agriculture and Agri-Food Canada, Winnipeg MB, Canada. Canada
Authors
Winnie LeungEastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa ON, Canada. Canada
Authors
Barbara BlackwellEastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa ON, Canada. Canada
Authors
Rajagopal Subramaniamsubramaniamra@agr.gc.ca
Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa ON, Canada. Canada
Statistics
Abstract views: 56PDF downloads: 33
License
All articles published in electronic form under CC BY-SA 4.0, in open access, the full content of the licence is available at: https://creativecommons.org/licenses/by-sa/4.0/legalcode.pl .
