Fusarium DNA levels as compared to mycotoxin levels in Finnish and Estonian grain samples.
T. Yli-Mattila
Molecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, 20014, Turku, Finland. (Finland)
S. Rämö
MTT Agrifood Research Finland, Laboratories, 31600 Jokioinen, Finland. (Finland)
R. Tanner
National Institute of Chemical Physics and Biophysics, 12618 Tallinn, Estonia. (Estonia)
H. Loiveke
Plant Production Department, Estonian Research Institute of Agriculture, 7550 Saku, Estonia (Estonia)
V. Hietaniemi
MTT Agrifood Research Finland, Laboratories, 31600 Jokioinen, Finland. (Finland)
Abstract
In the present work Fusarium DNA levels of deoxynivalenol (DON)-producing F. graminearum and F. culmorum and T-2/HT-2-producing F. langsethiae and F. sporotrichioides were compared to DON and T-2/HT-2 levels in Finnish (19 oat samples) and Estonian (3 oat, 8 wheat and 9 barley) grain samples of the year 2008. The Finnish oat samples were selected based on high (<870 ppb) DON levels. Some of them also contained high levels of zearalenone (ZEN). The main aim was to find out, which Fusarium species is the main DON producer in oats in Finland. In Estonian grain sam- ples all mycotoxin levels were relatively low and below the EU limits. The highest ZEN levels were found in oat in Satakunta in Finland.
This is the first study, in which F. graminearum has been found in Estonian grains. The correlation between F. graminearum DNA and DON levels in the combined Finnish and Estonian oat samples was higly significant (R2 = 0.88). In Finnish samples a significant correlation was also found between F. graminearum DNA and ZEN levels (R2 = 0.42). No correlation was found between F. culmorum DNA and DON levels in the combined Finnish and Estonian oat sam- ples. There was also a significant correlation between the combined T-2 and HT-2 and combined F. langsethiae and F. sporotrichioides levels (R2 = 0.38) in the combined Finnish and Estonian oat samples. According to our results F. graminearum is clearly the main DON producer in Finnish oat.
Keywords:
DON, Fusarium. culmorum, F. graminearum, TaqMan qPCR, T-2/HT-2References
AOAC Official Methods of analysis, Zearalenone in corn, p. 481, p. 499
Google Scholar
Hietaniemi V., Kontturi M., Rämö S., Eurola M., Kangas A., Niskanen M., Saastamoinen M. 2004 Contents of trichothecenes in oats during official variety, organic cultivation and nitrogen fertilization trials in Finland. Agricultural and Food Science 13: 54-67
Google Scholar
Hietaniemi V., Rämö S., Manninen P., Parikka P., Hankomäki J. 2009. The effect of cleaning and de-hulling on the trichothecene content in oats and barley. NordForsk Mould and Mycotoxin Seminar, April 14-15, Uppsala, Sweden. http://fou02.planteforsk.no/NordforskNetworkMycotox/PDFs/PDFs%20Uppsala%202009/ SariPresentation_April2009s.pdf.
Google Scholar
Jestoi M., Paavanen-Huhtala S., Parikka S., Yli-Mattila T. 2008. In vitro and in vivo mycotoxin production of Fusarium species isolated from Finnish grains. Archives of Phytopathology and Plant Protection 41: 545 -558.
Google Scholar
Jestoi M., Paavanen-Huhtala S., Uhlig S., Rizzo A., Yli-Mattila T 2004. Mycotoxins and cytotoxicity of Finnish Fusarium strains grown on rice cultures. In: Canty SM, Boring T, Wardwell J and Ward RW Eds., Proceedings of the 2nd International Symposium on Fusarium Head Blight; incorporating the 8th European Fusarium seminar; 2004, 11-15; Orlando, Fl, USA. Michigan State University, East Lansing, MI. pp. 405-409.
Google Scholar
Nicholson P., Chandler E., Draeger R.C., Gosman N.E., Simpson D.R., Thomsett M., Wilson A.H. 2003. Molecular tools to study epidemiology and toxicology of Fusarium head blight of cereals. European Journal of Plant Pathology 109: 691-703.
Google Scholar
Parikka P., Kangas A., Högnäsbacka M., Weckman, A. 2010. Punahomealttius ohra- ja kauralajikkeissa. Maataloustieteen päivät 2010 abstract, http://www.smts.fi/jul2010/poste2010/074.pdf, in Finnish.
Google Scholar
Romer Labs Application Brief: Rapid, Accurate Quantitation of OTA in Corn by HPLC-FLD, App.4-01- 031006, 2003
Google Scholar
Waalwijk C., Kastelein P., Vries I. de, Kerenyi, Z., Lee T. van der, Hesselink, T., Kema, G. 2003. Major changes in Fusarium spp. in wheat in the Netherlands. European Journal of Plant Pathology 109: 743- 754.
Google Scholar
Yli-Mattila T., Gagkaeva T., Ward T.J., Aoki T., Kistler H.C., O’Donnell, K. 2009b. A novel Asian clade within the Fusarium graminearum species complex includes a newly discovered cereal head blight pathogen from the Far East of Russia. Mycologia 101: 841-852.
Google Scholar
Yli-Mattila T., Parikka P, Lahtinen T., Ramo S., Kokkonen M., Rizzo A., Jestoi M., Hietaniemi V. 2009a. Fusarium DNA levels in Finnish cereal grains 107-138 p. In: Current Advances in Molecular Mycology, Gherbawy, Y., Mach, L. & Rai, M., Eds., Nova Science Publishers, Inc., New York:.
Google Scholar
Yli-Mattila T., Paavanen-Huhtala S., Parikka P., Hietaniemi V., Jestoi M., Gagkaeva T., Sarlin T., Haikara A., Laaksonen S., Rizzo A. 2008. Real-time PCR detection and quantification of Fusarium poae, F. graminearum, F. sporotrichioides and F. langsethiae as compared to mycotoxin production in grains in Finland and Russia. Archives of Phytopathology and Plant Protection 41: 243-260.
Google Scholar
Authors
T. Yli-MattilaMolecular Plant Biology, Department of Biochemistry and Food Chemistry, University of Turku, 20014, Turku, Finland. Finland
Authors
S. RämöMTT Agrifood Research Finland, Laboratories, 31600 Jokioinen, Finland. Finland
Authors
R. TannerNational Institute of Chemical Physics and Biophysics, 12618 Tallinn, Estonia. Estonia
Authors
H. LoivekePlant Production Department, Estonian Research Institute of Agriculture, 7550 Saku, Estonia Estonia
Authors
V. HietaniemiMTT Agrifood Research Finland, Laboratories, 31600 Jokioinen, Finland. Finland
Statistics
Abstract views: 81PDF downloads: 34
License
All articles published in electronic form under CC BY-SA 4.0, in open access, the full content of the licence is available at: https://creativecommons.org/licenses/by-sa/4.0/legalcode.pl .
