PHENOTYPIC AND MARKER ASSISTED EVALUATION FOR PATHOGENICITY AND AGGRESSIVENESS OF ROMANIAN FUSARIUM ISOLATES VS. WHEAT

M. Ittu

ittum@ricic.ro
National Research Development Institute Fundulea, 1 N. Titulescu street, Fundulea, jud. Calarasi, 915200, Romania (Romania)

L. Cana

National Research Development Institute Fundulea, 1 N. Titulescu street, Fundulea, jud. Calarasi, 915200, Romania (Romania)

M. Ciuca

National Research Development Institute Fundulea, 1 N. Titulescu street, Fundulea, jud. Calarasi, 915200, Romania (Romania)

C. Voaides

Faculty of Biotechnology of the Agronomical Science and Veterinary Medicine University (Romania)

P. Cornea

CBAB Biotehnol, 59 Marasti Blv, Bucharest, Romania (Romania)

Abstract

Pathogenicity and aggressiveness vs. wheat of 30 new Fusarium accessions, primarily F. graminearum, obtained  from random naturally infected grain samples of bread wheat, durum wheat and triticale collected across Romania,  phenotypically and by molecular tools has been investigated. A large variation of this trait, expressed as reduction of  coleoptiles length (% of control), in seedling stage, on average over three varieties, ranging from 2.1 to 30.9 % was  registered. Point field inoculations at anthesis of 90 Fusarium isolate x wheat varieties combinations, revealed also  the variability of several components of aggressiveness: severity (14.4-64.8%), AUDPC (104.9-527.1), and FDK 8.1-
43.7%, respectively.
Molecular techniques allowed identification of Fusarium species and the analysis of polymorphism within fungal  isolates. Moreover, the presence of TRI 5 gene involved in DON biosynthesis was detected in the majority of isolates.
Similarity between records obtained in seedling and adult stage for the most aggressive of Fusarium isolates,  suggests that phenotypic selection in conjunction with molecular tools could be a reliable method to select the appropriate pathogen strains for breeding of resistance.

Keywords:

aggressiveness, Fusarium graminearum, F. culmorum, molecular polymorphism, TRI 5 gene