Peroxidase and IAA oxidase activities during sink development in cotton seed.

Vraj Rabadia

Department of Biosciences, Saurashtra University, Rajkot 360 005. (India)

Vrinda Thaker

vrindathaker@yahoo.com
Department of Biosciences, Saurashtra University, Rajkot 360 005. (India)

YeshDev Singh

Department of Biosciences, Saurashtra University, Rajkot 360 005. (India)

Abstract

Seeds of the three cotton genotypes, H-6 and H-4 (Gossypium hirsutum),and V₇₉₇ (G. herbaceum) for their growth variations in IAA oxidase and peroxidase activities. The three genotypes varied in their final seed weight. The peroxidase analysis was done with four different hydrogen donors. Though the trends in peroxidase activitywere almost similar in the three genotypes, there was variation with different hydrogen donors. These was an inverse correlation between Cytoplasmic peroxidase and Wall bound peroxidase activity and rate of water uptake of the seed. The peroxidase activity showed up increasing levels only after the rate of water uptake decreased. However, IAA oxidase activity did not show any discernible trend with water content. The role of cytoplasmic and wall bound peroxidase in seed development is discussed in detail    

Keywords:

cotton, IAA oxidase, peroxidase, seed development