Identification of 1bl/1rs translocation in interspecific hybrids between Aegilops and Triticum.

Mirosław Tyrka

University of Agriculture in Lublin, Institute of Genetics and Plant Breeding, Akademicka 15, 20-934 Lublin, Poland (Poland)

Grażyna Stefanowska

University of Agriculture in Lublin, Institute of Genetics and Plant Breeding, Akademicka 15, 20-934 Lublin, Poland (Poland)

Czesław Tarkowski

University of Agriculture in Lublin, Institute of Genetics and Plant Breeding, Akademicka 15, 20-934 Lublin, Poland (Poland)

Abstract

The aim of studies was testing for presence of 1BL/1RS translocation in 17 lines derived from wide crosses of Aegilops ventricosa and Aegilops juvenalis with common and durum wheats, along with parental forms. PCR analyses detected translocated chromosome in Lanca and CZR1406 wheats and in 4 hybrid lines (JCPC, JCCP, JCC and JCCC). The lack of 1RS arm in lines (VGL and VGLL) derived from crosses with Lanca suggests that another chromosomal rearrangements occurred. We confirmed the usefulness of applied STS-PCR assays for fast and robust identification of 1BL/1RS translocation in breeding materials.

Keywords:

1BL/1RS translocation, Aegilops spp., STS-PCR, Triticum durum, Triticum aestivum