Selection of frost-tolerant cell lines from cell cultures of Solanum tuberosum L.
Muhammad Akbar Anjum
Department of Horticulture, Univ. College of Agriculture, Bahauddin Zakariya University, Multan 60800, Pakistan. (Pakistan)
Abstract
Fourteen hydroxyproline-resistant cell lines were selected by plating 7 days old cell suspensions of Solanum tuberosum L. cvs. Desiree and Maris Piper on a cell plating medium containing 5 or 10 mM hydroxyproline (hyp). Cell suspensions were either plated directly on selective media or after mutagenic treatment with gamma rays at a dose of 20 Gy or after freezing to –6°C. The frequency of resistant colonies varied from 0.15 to 0.35 x 10-6. Almost all the selected lines possessed increased levels of frost tolerance as compared to their non-selected controls except one indicating that hyp resistance and frost tolerance are not necessarily linked.
Keywords:
potato, Solanum, cell culture, proline, hydoxyproline resistance, frost toleranceAuthors
Muhammad Akbar AnjumDepartment of Horticulture, Univ. College of Agriculture, Bahauddin Zakariya University, Multan 60800, Pakistan. Pakistan
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