CHARACTERISTICS OF CRY1AB PROTEIN FROM BIOINSECTICIDES AND INSECT RESISTANT GM CROPS
Ewelina Żmijewska
GMO Controlling Laboratory, Plant Breeding and Acclimatization Institute– National Research Institute1 , Plant Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatization Institute– National Research Institute2 ; (Poland)
Anna Linkiewicz
a.linkiewicz@ihar.edu.plGMO Controlling Laboratory, Plant Breeding and Acclimatization Institute– National Research Institute1 , Plant Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatization Institute– National Research Institute2 ; (Poland)
Magdalena Żurawska-Zajfert
GMO Controlling Laboratory, Plant Breeding and Acclimatization Institute– National Research Institute1 , Plant Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatization Institute– National Research Institute2 ; (Poland)
Katarzyna Grelewska-Nowotko
GMO Controlling Laboratory, Plant Breeding and Acclimatization Institute– National Research Institute1 , Plant Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatization Institute– National Research Institute2 (Poland)
Sławomir Sowa
GMO Controlling Laboratory, Plant Breeding and Acclimatization Institute– National Research Institute1 , Plant Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatization Institute– National Research Institute2 ; (Poland)
Janusz Zimny
Plant Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatization Institute– National Research Institute (Poland)
Abstrakt
Biological insecticides are an effective method used in plant protection. One of the most widely used active substances in biological insecticides is Cry1Ab protein, which is toxic for lepidopteran insects. This protein is produced during bacterial sporulation by Bacillus thuringiensis. Other sources of Cry1Ab protein are genetically modified plants (GM) with expression of cry1Ab gene. Cry1Ab protein in both bioinsecticides and GM plants is present in the form of protoxin, which requires activation by enzymatic treatment in the gut of susceptible insects. So far, Cry1Ab mode of action is not fully understood, but there are 3 main concepts describing it. Two of them assume that a toxic protein after binding to receptors in the insect gut penetrates into the cells, causing pore formation in the gut, which leads to the death of the sensitive insect. In the third model Cry1Ab toxic action is a result of toxin-induced chemical processes initiating a cell death pathway. This work describes the structure and mode of action of Cry1Ab protein, present in biological insecticides and genetically modified plants.
Słowa kluczowe:
biological insecticide, Cry1Ab protein, Cry protein mode of action, GM plantBibliografia
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Autorzy
Ewelina ŻmijewskaGMO Controlling Laboratory, Plant Breeding and Acclimatization Institute– National Research Institute1 , Plant Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatization Institute– National Research Institute2 ; Poland
Autorzy
Anna Linkiewicza.linkiewicz@ihar.edu.pl
GMO Controlling Laboratory, Plant Breeding and Acclimatization Institute– National Research Institute1 , Plant Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatization Institute– National Research Institute2 ; Poland
Autorzy
Magdalena Żurawska-ZajfertGMO Controlling Laboratory, Plant Breeding and Acclimatization Institute– National Research Institute1 , Plant Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatization Institute– National Research Institute2 ; Poland
Autorzy
Katarzyna Grelewska-NowotkoGMO Controlling Laboratory, Plant Breeding and Acclimatization Institute– National Research Institute1 , Plant Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatization Institute– National Research Institute2 Poland
Autorzy
Sławomir SowaGMO Controlling Laboratory, Plant Breeding and Acclimatization Institute– National Research Institute1 , Plant Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatization Institute– National Research Institute2 ; Poland
Autorzy
Janusz ZimnyPlant Biotechnology and Cytogenetics Department, Plant Breeding and Acclimatization Institute– National Research Institute Poland
Statystyki
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