ION-EXCHANGE MEMBRANE CHROMATOGRAPHY AS AN ALTERNATIVE METHOD OF SEPARATION OF POTATO Y VIRUS
Krzysztof Treder
Plant Breeding and Acclimatization Institute, Department of Potato Protection and Seed Science, Bonin 3, 76-009 Bonin (Poland)
Bogumiła Zacharzewska
Plant Breeding and Acclimatization Institute, Department of Potato Protection and Seed Science, Bonin 3, 76-009 Bonin (Poland)
Agnieszka Przewodowska
Plant Breeding and Acclimatization Institute, Department of Potato Protection and Seed Science, Bonin 3, 76-009 Bonin (Poland)
Włodzimierz Przewodowski
Plant Breeding and Acclimatization Institute, Department of Potato Protection and Seed Science, Bonin 3, 76-009 Bonin (Poland)
Katarzyna Otulak
Warsaw University of Life Sciences-SGGW, Faculty of Agriculture and Biology, Dept. of Botany, Nowoursynowska ST 159, 02-776 Warsaw (Poland)
Abstrakt
Procedures of separation of virus particles from a plant material are multistage. Furthermore often they are difficult in terms of methodology and require use of expensive, highly specialist equipment and yield of separation is often low. The antigen obtained is often degraded and contains admixtures of other proteins. Therefore, generation of high quality and specificity antibodies based on such antigen is very difficult and quality of the antibodies has impact on reliability, sensitivity and unambiguity of results of immunodiagnostic tests (e.g. ELISA) that are currently conventionally used to detect vegetable viruses. In this study three conventionallyperformed methods of separation of potato virus Y (PVY) were compared and a method of separation based on membrane chromatography, as an alternative separation technique, has been presented. It has been demonstrated that in proper process conditions good quality virus preparation can be obtained.
Słowa kluczowe:
antigen, membrane techniques, immunodetection, virus purificationBibliografia
Burden C.S., Jin J., Podgornik A., Bracewell D.G. 2011. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 880: 82.
Google Scholar
Cerovská N., Filigarová M., Šubr Z. 1997. Optimalization of purification procedure for potato virus Y strain NN. Acta Virologica 41(1): 47–49.
Google Scholar
Chrzanowska M. 1991. New isolates of the necrotic strain of Potato virus Y (PVY N) found recently in Poland. Potato Res. 34: 179-182.
Google Scholar
Clark M. F., Adams A. M. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34:475-483.
Google Scholar
Downing, L.A., Bernstein, J.M., Walter, A. 1992. Active respiratory syncytial virus purified by ion-exchange chromatography: characterization of binding and elution requirements. J. Virol. Methods 38: 215–228.
Google Scholar
Ghosh R. 2002. Protein separation using membrane chromatography: opportunities and challenges. J. Chromatogr. A. 952: 13.
Google Scholar
Grunwald A.G., Shields M.S. 2001. Plasmid Purification Using Membrane-Based Anion-Exchange Chromatography. Anal. Biochem. 296(1): 1–4.
Google Scholar
Hammond J., Lawson R.H. 1988. An improved purification procedure for preparing potyviruses and cytoplasmic inclusions from the same tissues. Journal of Virological Methods 20: 203-217.
Google Scholar
Hühnlein A., Drechsler N., Steinbach P., Thieme T., Schubert J. 2013 Comparison of three methods for the detection of Potato virus Y in seed potato certification. J Plant Dis Protection 120: 57 69.
Google Scholar
Karger A., Bettin B., Granzow H., Mettenleiter T.C. 1998. Simple and rapid purification of alphaherpesviruses by chromatography on a cation exchange membrane. J. Virol. Methods 70: 219–224.
Google Scholar
Kovac K., Gutiérrez-Aguirre I., Banjac M., Peterka M., Poljsak-Prijatelj M., Ravnikar M., Mijovski J.Z., Schultz A.C., Raspor P. 2009. A novel method for concentrating hepatitis A virus and caliciviruses from bottled water. J. Virol. Methods, 162: 272.
Google Scholar
Laemmli U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259): 680-685.
Google Scholar
Nochumson S., Zhang S. Rapid Culture and Elution of Large Quantities of Plasmid DNA (100 mg) Using Anion Exchange Chromatography Membranes. Fifth Meeting of the American Society of Gene Therapy, 5 June 2002, Abstract No. 1031.
Google Scholar
Paul H. L. 1975. SDS-polyacrylamide gel electrophoresis of virion proteins as a tool for detecting the presence of virus in plants. II Examination of further virus-host combinations. Phtyopath. Z. 83: 303 – 310.
Google Scholar
Roudet-Tavert G., German-Retana S., Delaunay T., Delécolle B., Candresse T., Le Gall O. 2002. Interaction between potyvirus helper component-proteinase and capsid protein in infected plants. J. Gen. Virol. 83: 1765.
Google Scholar
Rupar M., Ravnikar M., Tušek-Žnidari M., Kramberger P., Glais L., Gutiérrez-Aguirre I. 2013. Fast purification of the filamentous Potato virus Y using monolithic chromatographic supports. J Chromatogr. A. 1272: 33-40.
Google Scholar
Singh R. P. 1978. Detection of Potato Viruses by Electrophoresis on Polyacrylamide Gels. Report of Planning Conference on Development in the control of potato virus diseases. International Potato Center. CIP, Lima, Peru. 130-137.
Google Scholar
Singh R.P., McLaren D.L., Nie X., Singh M. 2003. Possible escape of a recombinant isolate of Potato virus Y by serological indexing and methods of its detection. Plant Dis 87: 679-685.
Google Scholar
Smrekar F., Ciringer M., Peterka M., Podgornik A., Strancar A. 2008. Purification and concentration of bacteriophage T4 using monolithic chromatographic supports. J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 861: 177.
Google Scholar
Thornbury D.W., Hellmann G.M., Rhoads R.E., Pirone T.P. 1985. Purification and characterization of potyvirus helper component. Virology. 144: 260–267.
Google Scholar
Walin, L., Tuma, R., Thomas, G.J. Jr., Bamford, D.H. 1994. Purification of viruses and macromolecular assemblies for structural investigations using a novel ion exchange method. Virology 201:1–7.
Google Scholar
Wróbel S. 2014. Modification of ELISA by replacing incubation of microtiter plates in an incubator with their shaking in PVY, PVM and PLRV detection. American Journal of Potato Research 91(4): 354 – 362.
Google Scholar
Valkonen J. P. 2007. Viruses: Economical losses and bio - technological potential. In: Vreugdenhil D, Bradshaw J, Gebhardt C, Govers F, McKarren DKL, Taylor MA & Ross HA (Eds.). Potato biology and biotechnology – Advances and perspectives. Elsevier 619-641.
Google Scholar
Vincente T., Sousa M., Peixoto C., Mota J., Alves P., Carrondo M. 2008. Anion-exchange membrane chromatography for purification of rotavirus-like particles. Journal of Membrane Science 311: 270-283.
Google Scholar
Zahn V. 2004. Altered occurrence of viruses. Results of potato virus testing by LWK Hannover. Kartoffelbau 55: 394-397.
Google Scholar
Zhou, J. X. and Tressel, T. (2006), Basic Concepts in Q Membrane Chromatography for Large-Scale Antibody Production. Biotechnol Progress, 22: 341–349.
Google Scholar
Zhou J.X., T. Tressel, U. Gottschalk, F. Solamo, A. Pastor, S. Dermawan, Hong T., Reif O., Mora J., Hutchison F., Murphy M. 2006. New Q membrane scale-down model for process-scale antibody purification, J. Chromatogr. A 1134: 66-73.
Google Scholar
Autorzy
Krzysztof TrederPlant Breeding and Acclimatization Institute, Department of Potato Protection and Seed Science, Bonin 3, 76-009 Bonin Poland
Autorzy
Bogumiła ZacharzewskaPlant Breeding and Acclimatization Institute, Department of Potato Protection and Seed Science, Bonin 3, 76-009 Bonin Poland
Autorzy
Agnieszka PrzewodowskaPlant Breeding and Acclimatization Institute, Department of Potato Protection and Seed Science, Bonin 3, 76-009 Bonin Poland
Autorzy
Włodzimierz PrzewodowskiPlant Breeding and Acclimatization Institute, Department of Potato Protection and Seed Science, Bonin 3, 76-009 Bonin Poland
Autorzy
Katarzyna OtulakWarsaw University of Life Sciences-SGGW, Faculty of Agriculture and Biology, Dept. of Botany, Nowoursynowska ST 159, 02-776 Warsaw Poland
Statystyki
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