Optimization of micropropagation by different concentration of vitamins and sucrose in St. John's Wort (Hypericum Perforatum)
Sahar Khakpour
Department of Horticultural Sciences, Urmia University, Urmia, Iran (Iran, Islamic Republic of)
Alireza Motallebi-Azar
motallebiazar@gmail.comDepartment of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran (Iran, Islamic Republic of)
Bahman Hosseini
Department of Horticultural Sciences, Urmia University, Urmia, Iran (Iran, Islamic Republic of)
Saeede Alizadeh-Salte
Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran (Iran, Islamic Republic of)
Abbas Hasani
Department of Horticultural Sciences, Urmia University, Urmia, Iran (Iran, Islamic Republic of)
Abstrakt
In order to approach optimal micropropagation of Hypericum perforatum, it will be necessary to optimize shoot proliferation stage in in vitro culture. This study was conducted to investigate the effects of different concentrations of B group vitamins; Thiamine HCl, Pyridoxine HCl, Nicotinic acid (control and 100 fold of MS) and Sucrose (30 and 40 g.l-1) on shoot proliferation. For this purpose, Stems with one node were taken from in vitro shoots and cultured on MS medium. All cultures kept at 16h light/8h photoperiod and 25 ±2 oC in growth chamber. Results showed that the highest number of shoots and leaves were achieved when ex-plants cultured in media containing 40 g.l-1 sucrose with 100 fold of MS vitamins. The highest shoots and leaf length were obtained with medium supplemented with 30 g.l-1 sucrose. Nicotinic acid concentrations had an important role in length of the leaves. The highest number of nodes achieved in the media containing 40 g.l-1 sucrose with both concentrations of Nicotinic acid. After two month growing plantlets, light (LGN) and dark glands number (DGN) were counted. Maximum number of LGN was observed in the media containing 30 g.l-1 sucrose with 100 fold of Thiamine and Pyridoxine. However, The Highest number of DGN achieved in the media containing 40 g.l-1 sucrose with 100 fold of Thiamine or Pyridoxine. Increasing of sucrose and vitamins concentrations were efficiently improved in vitro proliferation and some morphological attributes without negative side effects. Therefore, use of high levels of sucrose and vitamins were useful on micro-propagation of Hypericum perforatum.
Słowa kluczowe:
Hypericum Perforatum, nicotinic acid, pyridoxine, shoot proliferation, sucrose, thiamineBibliografia
Abrahamian P., Kantharajah A. 2011. Effect of :Vitamins on in vitro organogenesis of plant. American journal of plant Sciences. 2: 669-674.
Bagdonaite E., Janulis V., Ivanauskas L., Labokas J. 2002. Ex situ studies on chemical and morphological variability of Hypericum perforatum L. in Lithuania. Biologija journal. 53: 63-70.
Bais P.H., Ravishankar G.A. 2002. Role of polyamines in the ontogeny of plants and their biotechnological applications. Plant Cell, Tissue and Organ Culture. 69: 1-34.
Barnes J., Anderson A., Phillipson D. 2001. St. John's wort (Hypericum perforatum L.)A review of its chemistry, pharmacology and clinical properties. Journal of Pharmacy and Pharmacology. 53: 583-600.
Barwale U.B., Kerns H.R., Widholm J.M. 1986. Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta. 167: 473-481.
Briskin D.P., Gawienowski M.C. 2001. Differential effects of light and nitrogen on production of hypericins and leaf glands in Hypericum perforatum. Plant Physiology Biochemistry. 39: 1075-1081.
Cellarova E., Daxnerova Z., Kimakova K., Haluskova J. 1994. The variability of the hypericin content in the regenerants of Hypericum perforatum. Acta Biotechnology. 14: 267-274.
Curtis J.D., Lersten N.R. 2002. Internal secretary structure in Hypericum (Gluciaceae): H. Perforatum L. and H. balearicum L. New Phytologist. 14: 571-580.
Farsad Akhtar N., Aharizad S., Mohammadi S.A., Motallebi-Azar A., Movafeghi A., Banan Khojasteh S.M. 2013. In vitro shoot regeneration and hypericin production in four hypericum perforatum genotypes. International Journal of Agriculture: Research and Review. 3: 887-893.
Farsad Akhtar N., Aharizad S., Mohammadi S.A., Motallebi-Azar A., Movafeghi A. 2014. The study of relationship between morphological traits and hypericins content in Hypericum perforatum. Technical Journal of Engineering and Applied Sciences. 4: 45-47.
Fuentes S.R.L., Calheiros M.B.P., Manetti-Filho J., Vieira, L.G.E. 2000. The effects of silver nitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora. Plant Cell, Tissue and Organ Culture. 60: 5–13.
Gadzovska S., Maury S., Ounnar S., Righezza M., Kascakova S., Refregiers M., Spasenoski M., Joseph C., Hagege D. 2005. Identification and quantification of hypericin and pseudohypericin in different Hypericum perforatum L. in vitro cultures. Plant Physiology Biochemistery. 43: 591-601.
George E.F. 1993. Plant propagation by tissue culture. part1. The technology ExegeticsEngland. Potato. Phd Thesis, Punjab Agricultural University Ludhiana (pb) India.
Hall R.D. 1999. Plant Cell Culture Protocols. Humana Press Ottowa.
Jo E.A., Park K.Y. 2009. In vitro sucrose concentration affect growth and acclimatization of Alocazia Amazonica plantlets. Plant Cell, Tissue and Organ culture. 96: 307-315.
Karhu S.T. 1997. Sugar use in relation to shoot induction by sorbitol and cytokinin in apple. Journal of AmericanSociety Horticultural Science. 122: 476–480.
Kazemiani S., Motallebi-Azar A. 2011. Increasing gland number and redpigments in St. John’s wort in vitro culture: Influence of mannitol, sucrose and hydrolyzed casein. African Journal of Biotechnology. 10: 10- 29.
Kirakosyan A., Hayashi H., Inoue K., Charchoglyan A., Vardapetyan H. 2000. Stimulation of the production of hypericins by mannan in Hypericum perforatum shoot cultures. Phytochemistry. 53: 345-348.
Mahna N., Motallebi-Azar A. 2007. In vitro micropropagation of apple cv. Golden Delicius. Comm. Appl. Bio. Sci. 72: 235-238.
Motallebi-Azar A., Kazemiani S., Kiumarsie F., Mohaddes N. 2011. Shoot proliferation from node explants of potato (Solanum tuberosum cv. Agria). II. effect of different concentrations of NH4NO3, hydrolyzed casein and BAP. Romanian Biotech. Let. 16: 6199-6204.
Motallebi-Azar A., Mahna N., Markafshi A.S. 2011. Shoot regeneration from leaf segments of peach × almond hybrid, GF677 rootstock. Advances in Agriculture Botanics. 3: 168-177.
Murch S.J., Choffe K.L., Victor J.K.R., Slimmon, T.Y., Krishnaraj S., Saxena P.K. 2000. Thidiazuroninduced plant regeneration from hypocotyl cultures of St. John s wort (Hypericum perforatum cv Anthos ). Plant Cell Reports. 19: 576-581.
Pourjabar A., Mohammadi S.A., Khosroshali M., Motallebi-Azar A., Ziai S.A. 2010. Optimization of in vitro culture of Milk Thistle and analysis of somaclonal variation by RAPD markers. J. Agri. Sci. 19: 1-14.
Radusiene J., Bagdonaite E. 2002. Phenotypic Variation in Hypericum Perforatum L. and H.maculatum crantz the Wild Populations Lithuania. The Haworth Press.
Roest S., Bokelmann G.S. 1975. Vegetative propagation of Chrysanthemum morifolium Ram. in vitro. Science Horticulture. 3: 317-330.
Samantaray S., Maiti M. 2010. Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant. Revista de Biologia Tropical. 59: 435-445.
Sanchez C.C., Prado B., Rabanal R.M. 2002. Antidepressant effects of the methanol extract of several Hypericum species from the Canary Islands. Journal of Ethnopharmacology. 79: 119-127.
Santarem E.R., Astarita L.V. 2003. Multiple shoot formation in Hypericum perforatum L. and hypericin production. Brazilian Journal of Plant Physiology. 15: 43-47.
Wojcik A., Podstolski A. 2007. Leaf explant response in in vitro culture of St. John s wort (Hypericum perforatum L.). Acta Physiology Plant. 29: 151-156.
Zdunek K., Alfermann W. 1992. Initiation of shoot organ cultures of Hypericum perforatum and formation of hypericin derivatives. Planta Medica. 58: 621-625.
Zobayed S.M.A., Murch S.J., Rupasinghe H.P.V., Saxen P.K. 2004. In vitro production and chemical characterization of St. John,s wort (Hypericum perforatum L. cv "New Stem"). Plant Science. 166: 333- 340.
Zolfagari-Nasab R., Khosroshahli M., Girigorian V., Motallebi-Azar A. 2004. Investigation on in vitro propagation of apricot x Plum natural hybrid. Iranian J. Hort. Sci. Tech. 5: 81-92. Google Scholar
Bagdonaite E., Janulis V., Ivanauskas L., Labokas J. 2002. Ex situ studies on chemical and morphological variability of Hypericum perforatum L. in Lithuania. Biologija journal. 53: 63-70.
Bais P.H., Ravishankar G.A. 2002. Role of polyamines in the ontogeny of plants and their biotechnological applications. Plant Cell, Tissue and Organ Culture. 69: 1-34.
Barnes J., Anderson A., Phillipson D. 2001. St. John's wort (Hypericum perforatum L.)A review of its chemistry, pharmacology and clinical properties. Journal of Pharmacy and Pharmacology. 53: 583-600.
Barwale U.B., Kerns H.R., Widholm J.M. 1986. Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta. 167: 473-481.
Briskin D.P., Gawienowski M.C. 2001. Differential effects of light and nitrogen on production of hypericins and leaf glands in Hypericum perforatum. Plant Physiology Biochemistry. 39: 1075-1081.
Cellarova E., Daxnerova Z., Kimakova K., Haluskova J. 1994. The variability of the hypericin content in the regenerants of Hypericum perforatum. Acta Biotechnology. 14: 267-274.
Curtis J.D., Lersten N.R. 2002. Internal secretary structure in Hypericum (Gluciaceae): H. Perforatum L. and H. balearicum L. New Phytologist. 14: 571-580.
Farsad Akhtar N., Aharizad S., Mohammadi S.A., Motallebi-Azar A., Movafeghi A., Banan Khojasteh S.M. 2013. In vitro shoot regeneration and hypericin production in four hypericum perforatum genotypes. International Journal of Agriculture: Research and Review. 3: 887-893.
Farsad Akhtar N., Aharizad S., Mohammadi S.A., Motallebi-Azar A., Movafeghi A. 2014. The study of relationship between morphological traits and hypericins content in Hypericum perforatum. Technical Journal of Engineering and Applied Sciences. 4: 45-47.
Fuentes S.R.L., Calheiros M.B.P., Manetti-Filho J., Vieira, L.G.E. 2000. The effects of silver nitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora. Plant Cell, Tissue and Organ Culture. 60: 5–13.
Gadzovska S., Maury S., Ounnar S., Righezza M., Kascakova S., Refregiers M., Spasenoski M., Joseph C., Hagege D. 2005. Identification and quantification of hypericin and pseudohypericin in different Hypericum perforatum L. in vitro cultures. Plant Physiology Biochemistery. 43: 591-601.
George E.F. 1993. Plant propagation by tissue culture. part1. The technology ExegeticsEngland. Potato. Phd Thesis, Punjab Agricultural University Ludhiana (pb) India.
Hall R.D. 1999. Plant Cell Culture Protocols. Humana Press Ottowa.
Jo E.A., Park K.Y. 2009. In vitro sucrose concentration affect growth and acclimatization of Alocazia Amazonica plantlets. Plant Cell, Tissue and Organ culture. 96: 307-315.
Karhu S.T. 1997. Sugar use in relation to shoot induction by sorbitol and cytokinin in apple. Journal of AmericanSociety Horticultural Science. 122: 476–480.
Kazemiani S., Motallebi-Azar A. 2011. Increasing gland number and redpigments in St. John’s wort in vitro culture: Influence of mannitol, sucrose and hydrolyzed casein. African Journal of Biotechnology. 10: 10- 29.
Kirakosyan A., Hayashi H., Inoue K., Charchoglyan A., Vardapetyan H. 2000. Stimulation of the production of hypericins by mannan in Hypericum perforatum shoot cultures. Phytochemistry. 53: 345-348.
Mahna N., Motallebi-Azar A. 2007. In vitro micropropagation of apple cv. Golden Delicius. Comm. Appl. Bio. Sci. 72: 235-238.
Motallebi-Azar A., Kazemiani S., Kiumarsie F., Mohaddes N. 2011. Shoot proliferation from node explants of potato (Solanum tuberosum cv. Agria). II. effect of different concentrations of NH4NO3, hydrolyzed casein and BAP. Romanian Biotech. Let. 16: 6199-6204.
Motallebi-Azar A., Mahna N., Markafshi A.S. 2011. Shoot regeneration from leaf segments of peach × almond hybrid, GF677 rootstock. Advances in Agriculture Botanics. 3: 168-177.
Murch S.J., Choffe K.L., Victor J.K.R., Slimmon, T.Y., Krishnaraj S., Saxena P.K. 2000. Thidiazuroninduced plant regeneration from hypocotyl cultures of St. John s wort (Hypericum perforatum cv Anthos ). Plant Cell Reports. 19: 576-581.
Pourjabar A., Mohammadi S.A., Khosroshali M., Motallebi-Azar A., Ziai S.A. 2010. Optimization of in vitro culture of Milk Thistle and analysis of somaclonal variation by RAPD markers. J. Agri. Sci. 19: 1-14.
Radusiene J., Bagdonaite E. 2002. Phenotypic Variation in Hypericum Perforatum L. and H.maculatum crantz the Wild Populations Lithuania. The Haworth Press.
Roest S., Bokelmann G.S. 1975. Vegetative propagation of Chrysanthemum morifolium Ram. in vitro. Science Horticulture. 3: 317-330.
Samantaray S., Maiti M. 2010. Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae), an endangered medicinal plant. Revista de Biologia Tropical. 59: 435-445.
Sanchez C.C., Prado B., Rabanal R.M. 2002. Antidepressant effects of the methanol extract of several Hypericum species from the Canary Islands. Journal of Ethnopharmacology. 79: 119-127.
Santarem E.R., Astarita L.V. 2003. Multiple shoot formation in Hypericum perforatum L. and hypericin production. Brazilian Journal of Plant Physiology. 15: 43-47.
Wojcik A., Podstolski A. 2007. Leaf explant response in in vitro culture of St. John s wort (Hypericum perforatum L.). Acta Physiology Plant. 29: 151-156.
Zdunek K., Alfermann W. 1992. Initiation of shoot organ cultures of Hypericum perforatum and formation of hypericin derivatives. Planta Medica. 58: 621-625.
Zobayed S.M.A., Murch S.J., Rupasinghe H.P.V., Saxen P.K. 2004. In vitro production and chemical characterization of St. John,s wort (Hypericum perforatum L. cv "New Stem"). Plant Science. 166: 333- 340.
Zolfagari-Nasab R., Khosroshahli M., Girigorian V., Motallebi-Azar A. 2004. Investigation on in vitro propagation of apricot x Plum natural hybrid. Iranian J. Hort. Sci. Tech. 5: 81-92. Google Scholar
Khakpour, S., Motallebi-Azar, A., Hosseini, B., Alizadeh-Salte, S., & Hasani, A. (2015). Optimization of micropropagation by different concentration of vitamins and sucrose in St. John’s Wort (Hypericum Perforatum). Plant Breeding and Seed Science, 71, 67–79. Pobrano z http://ojs.ihar.edu.pl/index.php/pbss/article/view/266
Autorzy
Sahar KhakpourDepartment of Horticultural Sciences, Urmia University, Urmia, Iran Iran, Islamic Republic of
Autorzy
Alireza Motallebi-Azarmotallebiazar@gmail.com
Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran Iran, Islamic Republic of
Autorzy
Bahman HosseiniDepartment of Horticultural Sciences, Urmia University, Urmia, Iran Iran, Islamic Republic of
Autorzy
Saeede Alizadeh-SalteDepartment of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran Iran, Islamic Republic of
Autorzy
Abbas HasaniDepartment of Horticultural Sciences, Urmia University, Urmia, Iran Iran, Islamic Republic of
Statystyki
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