Pollen morphology and viability relates to seed productionin hybrid roses
Muhammad Nadeem
nadeem_daisy905@yahoo.comInstitute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan (Pakistan)
Masum Akond
Plant Genomics and Biotechnology Lab, Department of Biological Sciences, Fayetteville State University, Fayetteville, NC-28301 (United States)
Atif Riaz
Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan (Pakistan)
Muhammad Qasim
Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan (Pakistan)
Adnan Younis
Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan (Pakistan)
Amjad Farooq
Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan (Pakistan)
Abstrakt
To avoid the risk of very low seed production, the estimation of the pollen fertility is the reliable tool. In the present study nine hybrid rose genotypes were evaluated for their pollen viability and fertility status in vitro. The strength of contribution of each cultivar as a male parent was evaluated in the field. Successful crossing %age was correlated with the pollen germination %age and pollen tube length of each male parent cultivars in controlled conditions. Pollen vigor parameters indicated great variations in pollen quality, quantity, and viability and germination % ages. On average basis the performance of the cultivar Angel Face and Handel was better with respect to both number of anther per flower and number of pollens per anther. Pollen size (L: D) was almost same in all cultivars hence no significant differences were observed. Comparison of means of viability %age revealed that maximum number of viable pollens in term of viability %age were possessed by cultivar Handel (70%) followed by ‘Gruss an Teplitz’ (64%). The minimum viability of pollens was exhibited by the Cultivar ‘Autumn Sunset’ having pollen viability of 35%. A strong correlation among pollen germination %age and pollen tube length (r = 0.77) and pollen germination %age and pollen viability %age (r = 0.731) was exhibited. Pollen germination is also strongly correlated with pollen size (r =0.738) The comparison of various fertility parameters in vitro and in vivo revealed that cultivar “Handel” showing its maximum pollen viability in vitro performed well to get more crossing success in the field .
Słowa kluczowe:
crossing, fertility, pollen grains, rosa × hybridaBibliografia
Arzani, A., Poursiahbidi, M., and Mortazavi S. E. 2000. An acetocarmine staining procedure for chromosome banding studies of immature pollen in Triticeae. J. Agr. Sci. Tech., 2: 167-175
Google Scholar
Calvino, E.M. 1951. Ricerche sul pollini del genera Rosa. Ann . Sperimentazio Agraria, 5: 377-407.
Google Scholar
Crespel, L., Ricci, S., and Gudin, S. 2006. The production of 2n pollen in rose. Euphytica, 151: 155-164.
Google Scholar
DeVries, D.P., and Dubois, L.A.M. 1987. The effect of temperature on fruit set, seed set and seed germination in Sonia x Hadley hybrid Tea-rose crosses. Euphytica, 36:117-120.
Google Scholar
Darwin, C. 1884. The different forms of flowers on plants of the same species, 2nd Edn., Murray, J, London.
Google Scholar
DeVries, D.P., Dubois and L.A.M. 1996. Rose breeding: past, present, prospects. Acta Hort., 420: 241-248.
Google Scholar
Ercisli, S. 2007. Determination of pollen viability and in vitro pollen germination of Rosa dumalis and Rosa vilosa. Bangladesh J. Bot., 36(2): 185-187.
Google Scholar
Erlanson, E.W. 1931. Sterility in wild roses and in some species hybrids. Genetics, 16: 75-96.
Google Scholar
Eti, S. 1990. A practical method for the determination of pollen production. J. Agricul. Faculty Cukurova University, 5: 49-58.
Google Scholar
Faegririand K., and Van der Pijl, L. 1979. The Principles of Pollination Ecology, Pergamon Press, Oxford.
Google Scholar
Gudin, S., and Arene, L. 1991. Influence of the pH of the stigmatic exudate on male-female interaction in Rosa hybrida L. Sexual Plant Reproduction, 4: 110-112.
Google Scholar
Gudin S., and Arene, L. 1992. Putrescine increases effective pollination period in roses. Hort Technology, 2: 211-213.
Google Scholar
Gudin, S. 1995. Rose improvement: a breeder’s experience. Acta Hort., 420: 125-128.
Google Scholar
Gudin, S. 2000. Rose: Genetics and breeding. In: J. Janick (ed.), Plant Breeding Reviews. John Wiley & Sons, Inc., pp.159-189.
Google Scholar
Gudin, S., Arene L., and Chavagnat, A. 1992. Relation entre imbibition, densite, taux de remplissage et faculte ´ germinative chez l’ake`ne de Rosa hybrida L. Agronomie, 12: 123-126.
Google Scholar
Gudin, S., Arene, L., and Bulard, C. 1991a. Influence of season on pollen quality. Sexual Plant Reproduction 4:113-117.
Google Scholar
Gudin, S., Arene, L., Pellegrino, C. 1991b. Influence of temperature and hygrometry on rose pollen germination. Adv Hortic Sci., 5: 96-98.
Google Scholar
Gudin, S. 1992. Influence of bud chilling on subsequent reproductive fertility in roses. Sci Hortic, 51:139-144.
Google Scholar
Gunes, M., Cekic C., and Edizer, Y. 2005. Determination of pollen quantity, pollen viability and pollen germination in some dogrose species (Rosa section Caninae). Acta Hort., 690: 211-217.
Google Scholar
Jacob ,Y., Pierret, V. 2000. Pollen size and ploidy level in the genus Rosa. Acta Hort., 508: 289-292.
Google Scholar
Javaday, T., and Arzani, K. 2001. Pollen morphology of five Iranian Olive (Olea europaea L.) cultivars. J. Agric. Sci. Technol., 3: 37-42
Google Scholar
Khan, M.A .1988. Studies on the compatibility of six hybrid tea roses. Pak. J. Agric. Sci., 23: 73-78.
Google Scholar
Koncalova, M.N. 1975. Studies in Rosa pollen-I: Invitro germination of pollen grains of Rosa hugonsis. Preslia 47:22-25.
Google Scholar
Lee, T.D. 1988. Patterns of fruit and seed production. In: J.L. Doust, L.L. Doust (ed.), Plant Reproductive Ecology- Patterns and Strategies Oxford: Oxford University Press 179-202.
Google Scholar
Leus, L. 2005. Resistance breeding for powdery mildew (Podosphaera pannosa) and black spot (Diplocarpon rosae) in roses. PhD Thesis, Faculty of Bioscience Engineering, Ghent University, Belgium.
Google Scholar
Lloyd, D.G., and Schoen, D.J. 1992. Self and Cross fertilization in Plants. I. Functional dimensions. Int J Plant Sci., 153: 358-369.
Google Scholar
Morey D 1959. Observations on the genetics of doubleness in roses. American Rose Annul 44: 113–116.
Google Scholar
Mortazavi, S.M.H. Arzani, K., and Moieni, A. 2010. Optimizing storage and in vitro germination of Date Palm (Phoenix dactylifera) pollen. J. Agr. Sci. Tech., 12: 181-189
Google Scholar
Ogilvie, I., Cloutier, D., Arnold, N., and Jui, P.Y. 1991. The effect of gibberellic acid on fruit and seed set in crosses of garden and winter hardy Rosa accessions. Euphytica, 52: 119-123.
Google Scholar
Pipino, L., Marie-Christine, V.L., Andrea, M., Valentina, S., Annalisa, G., and Leen, L. 2010. Pollen morphology as fertility predictor in hybrid tea roses. Euphytica. DOI 10.1007/s10681-010-0298-5.
Google Scholar
Proctor, M., Yeo, P., and Lack, A. 1996. The natural History of Pollination. Portland, Oregon: Timber Press.
Google Scholar
Richer, C., Poulin, M., and Rioux, J.A. 2007. Factor influencing pollen germination in Explorer roses. Can J Plant Sci., 87: 115-119.
Google Scholar
Roberts, A.V. 1979. Science and roses. The Rose Annual. England: The Royal National Rose Society, 140- 146p.
Google Scholar
Stephenson, A.G. 1981. Flower and fruit abortion: proximate causes and ultimate functions. Annu. Rev. Ecol., 12: 253-279.
Google Scholar
Ueda, Y., and Akimoto, S. 2001. Cross and self compatibility in various species of the genus Rosa. J Hortic Sci Biotech., 76: 392-395.
Google Scholar
Vasil’eva, Y.O. 2009. Reproduction systems of representatives of the Genus Rosa L. under conditions of continental Climate. Cont. Prob. Ecol., 4: 361-368.
Google Scholar
Visser, T., DeVries, D.P., and Scheurink, J.A.M., and Welles, G.W.H. 1977a. Hybrid tea-rose pollen I. Germination and storage. Euphytica 26: 721-728.
Google Scholar
Visser T, DeVries, D.P., Welles, G.W.H., and Scheurink, J.A.M. 1977b. Hybrid tea-rose pollen II. Inheritance of pollen viability. Euphytica 26: 729-732.
Google Scholar
Voyiatzi, C.I. 1995. An assessment of the in vitro germination capacity of pollen of five tea hybrid rose cultivars. Euphytica 83: 199-204.
Google Scholar
Werlemark, G. 2000. Evidence of Apomixis in hemisexual dog roses, Rosa section caninae. Sexual Plant Reproduction 12: 353-359.
Google Scholar
Zlesak, D.C. 2006. Rosa x hybrida. In: N.O. Anderson (ed.), Flower Breeding and Genetics, The Netherlands, Springer, pp. 695-738.
Google Scholar
Zlesak, D.C., Zuzek, K., and Hokanson, S.C. 2007. Rose pollen viability over time at varying storage temperatures. Acta Hort., 751:337-343.
Google Scholar
Zlesak, D.C. 2009. Pollen diameter and guard cell length as predictors of ploidy in diverse rose cultivars, species and breeding lines. In: J.A. Taxeira da Silva (ed.), Floriculture and ornamental biotechnology London: Global Science Books, pp. 53-70.
Google Scholar
Autorzy
Muhammad Nadeemnadeem_daisy905@yahoo.com
Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan Pakistan
Autorzy
Masum AkondPlant Genomics and Biotechnology Lab, Department of Biological Sciences, Fayetteville State University, Fayetteville, NC-28301 United States
Autorzy
Atif RiazInstitute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan Pakistan
Autorzy
Muhammad QasimInstitute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan Pakistan
Autorzy
Adnan YounisInstitute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan Pakistan
Autorzy
Amjad FarooqInstitute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan Pakistan
Statystyki
Abstract views: 176PDF downloads: 0
Licencja
Wszystkie artykuły publikowane w formie elektronicznej na mocy licencji CC BY-SA 4.0, w otwartym dostępie (open access), pełna treść licencji jest dostępna pod adresem: https://creativecommons.org/licenses/by-sa/4.0/legalcode.pl .
