Genetic diversity of common bean genotypes as revealed by seed storage proteins and some agronomic traits
Akbar Marzooghian
marzooghian@tabrizu.ac.irDepartment of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran (Iran, Islamic Republic of)
Mohammad Moghaddam
Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran (Iran, Islamic Republic of)
Mostafa Valizadeh
Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran (Iran, Islamic Republic of)
Mohammad Hasan Kooshki
Broujerd Agricultural Research Station, Broujerd, Iran (Iran, Islamic Republic of)
Abstrakt
Evaluation of the genetic diversity present within species is essential for conservation, management and utilization of the genetic resources. The objective of this study was to evaluate genetic variability of 70 com- mon bean genotypes for seed storage proteins, grain morphological characteristics and agronomic traits. Two methods of extracting soluble seed proteins in salt were used.
Positive correlations were observed among both seed morphological characters and developmental charac- ters while yield components showed negative correlations with each other. Factor analysis for agronomic and grain morphological traits resulted in three factors were named yield components, seed morphology and phenology, respectively. Most genotypes had lower or medium scores for yield components and phenology factors. Considerable diversity was observed for seed morphology factor among the common bean genotypes.
Nei’s diversity coefficient (He = 0.4), effective number of alleles (Ae = 1.69) and number of polymorphic loci (N = 17) indicated larger variation in the extraction method of soluble proteins in low salt (0.2 M NaCl) than high salt (1 M NaCl) condition. Considering that the centers of diversity for common bean are different in seed size, the result of GST statistics showed that bands with relative mobility of 30, 32, 38 and 40 differen- tiated two weight groups more than other bands. Furthermore, significant differences were observed between these bands for number of pods per plant and number of seeds per plant.
Słowa kluczowe:
agronomic traits, genetic diversity, Phaseolus vulgaris L., SDS-PAGE, seed storage proteinsBibliografia
Barelli MAA, Celeste M, Vidigal Filho PS, Scapim, CA. (2000) Combining ability among six common bean cultivars adapted to the North West Region of Paraná State, Brazil. Bragantia. 59: 159-164.
Google Scholar
Beebe SS, Tohme P, Duque J, Pedraza M, Nienhuis F. (2000) Structure of genetic diversity among common bean landraces of Middle American origin based on correspondence analysis of RAPD. Crop Sci. 40, 264-273.
Google Scholar
Blair M, Giraldo M, Buendía H, Tovar E, Duque M, Beebe S. (2006) Microsatellite marker diversity in com- mon bean (Phaseolus vulgaris L.). Theor Appl Genet. 113, 100-109.
Google Scholar
Brown JWC, Bliss FA, Hall TC. (1981 a) Linkage relationships between genes controlling seed proteins in French beans. Theor Appl Genet. 60:251-259.
Google Scholar
Brown JWS, Ma Y, Bliss FA, Hall TC. (1981 b) Genetic variation in the subunits of globulin-1 storage pro- tein of French bean. Theor Appl Genet. 59:83-88.
Google Scholar
Castiñeiras L, Esquivel M, Lioi L, Hammer K. (1991) Origin, diversity and utilization of the Cuban germ- plasm of common bean (Phaseolus vulgaris L.). Euphytica. 57, 1-8.
Google Scholar
Corte AD, Moda-Cirino V, Arias CAA, Toledo JFF, Destro D. (2010) Genetic analysis of seed morphological traits and its correlations with Grain yield in common bean. Braz Arch Biol Technol. 53, 27-34.
Google Scholar
Durán L, Blair M, Giraldo M, Machiavelli R, Prophete E, Nin J, Beaver J. (2005) Morphological and molecu- lar characterization of common bean (Phaseolus vulgaris L.) landraces from the Caribbean. Crop Sci. 45, 1320-1328.
Google Scholar
Evans A. (1980) Structure, variation, evolution, and classification in Phaseolus. Advances in legume science (Summerfield, RJ; Bunting, AH [Editors]). p. 337-347. FAO. Production Yearbook. Vol. 56. Rome. 2003.
Google Scholar
Gepts P, Osborn T, Rashka K, Bliss F. (1986) Phaseolin-protein variability in wild forms and landraces of the common bean (Phaseolus vulgaris): evidence for multiple centers of domestication. Econ Bot. 40, 451- 468.
Google Scholar
Gonçalves-Vidigal MC, Silvero L, Elias HT, Filho, PSV. (2008) Combining ability and heterosis in common bean cultivars. Pesq agropec bras. 43, 1143-1150.
Google Scholar
Hames B, Richwood D. (1990) Gel electrophoresis of proteins: a practical approach. Oxford University Press, USA.
Google Scholar
Khairallah M, Adams M, Sears B. (1990) Mitochondrial DNA polymorphisms of Malawian bean lines: fur- ther evidence for two major gene pools. Theor Appl Genet. 80, 753-761.
Google Scholar
Krochko JE, Bewley DJ. (2000) Seed storage proteins in cultivars and subspecies of alfalfa (Medicago sativa L.). Seed Sci Res. 10, 423-434.
Google Scholar
Laemmli UK. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. nature. 227, 680-685.
Google Scholar
Maciel FL, Echeverrigaray S, Sheng Gerald LT, Grazziotin FG. (2003) Genetic relationships and diversity among Brazilian cultivars and landraces of common beans (Phaseolus vulgaris L.) revealed by AFLP markers. Genet Resour Crop Ev. 50, 887-893.
Google Scholar
Martin GB, Adams MW. (1987a) Landraces of Phaseolus vulgaris (Fabaceae) in northern Malawi. I. Regional variation. Econ Bot. 41, 190-203.
Google Scholar
Martin GB, Adams MW. (1987b) Landraces of Phaseolus vulgaris (Fabaceae) in northern Malawi. II. Genera- tion and maintenance of variability. Econ Bot. 41, 204-215.
Google Scholar
McClean P, Kami J, Gepts P. (2004) Genomics and genetic diversity in common bean. Legume Crop Genom- ics. AOCS Press, Champaign. p. 60–82.
Google Scholar
Miklas P. (2000) Use of Phaseolus germplasm in breeding pinto, great northern, pink, and red bean for the Pacific Northwest and intermountain region. p. 13–29 in Singh SP (ed.) Bean Research, Production and Utilization. Proc. of the Idaho Bean Workshop. Univ. of Idaho.
Google Scholar
Mitranov L. (1983) A study of general and specific combining ability for productivity in kidney bean (Phaseolus vulgaris L.) cultivars. Genet Plant Breed (Sofia). 16, 176-180.
Google Scholar
Nienhuis J, Singh S. (1988) Genetics of Seed Yield and its Components in Common Bean (Phaseolus vulgaris L.) of Middle-American Origin. Plant breeding. 101, 155-163.
Google Scholar
Pereira T, Coelho CMM, Bogo A, Guidolin AF, Miquelluti DJ. (2009) Diversity in common bean landraces from south Brazil. Acta Bot Croatica. 68, 79-92.
Google Scholar
Pirbalouti AG, Golparvar, AR, Rostampoor SA. (2006) Evaluation of seed yield and yield components of common bean Iranian cultivars for inoculation with four strains of Rhizobium legominosarum biovar Phaseoli. J. Agron. 5, 382-386.
Google Scholar
Rodiño A, González M, De Ron AM, Singh AM, Shree P. (2006) Novel genetic variation in common bean from the Iberian Peninsula. Crop Sci. 46, 2540-2546.
Google Scholar
Rodiño A, Santalla M, De Ron A, Singh S. (2003) A core collection of common bean from the Iberian penin- sula. Euphytica. 131, 165-175.
Google Scholar
Rodiño A, Santalla M, Montero I, Casquero P, De Ron A. (2001) Diversity of common bean (Phaseolus vulgaris L.) germplasm from Portugal. Genet Resour Crop Ev. 48, 409-417.
Google Scholar
Santalla M, Menéndez-Sevillano MC, Monteagudo AB, De Ron AM. (2004) Genetic diversity of Argentinean common bean and its evolution during domestication. Euphytica. 135, 75-87.
Google Scholar
Santalla M, Rodiño A, De Ron A. (2002) Allozyme evidence supporting southwestern Europe as a secondary center of genetic diversity for the common bean. Theorl Appl Genet. 104, 934-944.
Google Scholar
Singh SP, Gepts P, Debouck DG. (1991a) Races of common bean (Phaseolus vulgaris, Fabaceae). Econ Bot. 45, 379-396.
Google Scholar
Singh SP, Gutierrez J, Molina A, Urrea C, Gepts P. (1991b) Genetic diversity in cultivated common bean: II. Marker-based analysis of morphological and agronomic traits. Crop Sci. 31, 23-29.
Google Scholar
Singh SP. (1992) Common bean improvement in the tropics. Plant Breeding Rev. 10, 199-269.
Google Scholar
Singh SP. (1999) Production and utilization. pp 1-24 in Singh, S.P. (ed) Common bean improvement in the twenty-first century. London, Kluwer.
Google Scholar
Singh SP. (2001) Broadening the genetic base of common bean cultivars. Crop Sci. 41, 1659-1675.
Google Scholar
Stoilova T, Pereira G, de Sousa M, Carnide V. (2005) Diversity in common bean landraces (Phaseolus vul- garis L.) from Bulgaria and Portugal. J Cent Eur Agri. 6, 443-448.
Google Scholar
Tiwari M, Singh N, Rathore M, Kumar N. (2005) RAPD markers in the analysis of genetic diversity among common bean germplasm from Central Himalaya. Genet Resour Crop Ev. 52, 315-324.
Google Scholar
Voysest O, Valencia MC, Amezquita MC. (1994) Genetic diversity among Latin American Andean and Mesoamerican common bean cultivars. Crop Sci. 34, 1100-1110.
Google Scholar
Workman P, Niswander J. (1970) Population studies on southwestern Indian tribes. II. Local genetic differen- tiation in the Papago. Am J Hum Genet. 22, 24-29.
Google Scholar
Autorzy
Akbar Marzooghianmarzooghian@tabrizu.ac.ir
Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran Iran, Islamic Republic of
Autorzy
Mohammad MoghaddamDepartment of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran Iran, Islamic Republic of
Autorzy
Mostafa ValizadehDepartment of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran Iran, Islamic Republic of
Autorzy
Mohammad Hasan KooshkiBroujerd Agricultural Research Station, Broujerd, Iran Iran, Islamic Republic of
Statystyki
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