Biomolecular characterization of Fusarium poae strains isolated from durum wheat in central Italy.
I. Alberti
ENSE - Ente Nazionale Sementi Elette, Via Ca’ Nova Zampieri 37, S. Giovanni Lupatoto, Verona, Italy. (Italy)
Dal M. Prà
ENSE - Ente Nazionale Sementi Elette, Via Ca’ Nova Zampieri 37, S. Giovanni Lupatoto, Verona, Italy. (Italy)
S. Tonti
ENSE - Ente Nazionale Sementi Elette, Via Ca’ Nova Zampieri 37, S. Giovanni Lupatoto, Verona, Italy. (Italy)
M. Montanari
ENSE, Via Sicilia 2, Osteria Grande, Bologna, Italy. (Italy)
A. Prodi
Department of Agroenvironmental Science and Technology (DiSTA) – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale Fanin 40, Bologna, Italy. (Italy)
D. Pancaldi
Department of Agri-food Protection and Improvement (DIPROVAL), Alma Mater Studiorum University of Bologna, Viale Fanin 46, Bologna, Italy. (Italy)
Abstrakt
Fusarium Head Blight (FHB) is a worldwide disease affecting wheat, barley and other grains, reducing kernel weight and grain yield; infected seeds may contain a large number of mycotoxins, including trichothe- cenes of type A and B. These compounds have already been associated with human and animal toxicoses.
Most common species causing the disease are F. graminearum, F. culmorum and F. avenaceum, but in the last few years a gradual increase in incidence of another species, F. poae, has been reported. In general terms, F. poae is a relatively weak pathogen, but its contribute to the increase of mycotoxins level still has to be clarified.
Durum wheat is widely cultivated in the central part of Italy, however the effective incidence of F. poae in this area still has to be investigated.
In order to monitor Fusarium risk, we collected dozens of F. poae strains on seeds and glumes of durum wheat coming from some of the most important cultivated areas of Central Italy. Every isolate was identified both by microscope observation and by PCR assay with the primer pair Fp82 F/R.
Strains were therefore subjected to a more accurate molecular characterization by Translation Elongation Factor 1-alpha (TEF-1α) gene sequencing.
Słowa kluczowe:
durum wheat, FHB, F. poae, gene sequencing, mycotoxins, trichothecenesBibliografia
Bourdages J.V., Marchand S., Rioux S., Belzile F.J., 2006 Diversity and prevalence of Fusarium species from Quebec barley fields. Canadian Journal of Plant Pathology 28, 419-425.
Google Scholar
Geiser D. M., Jiménez-Gasco M. d. M., Kang S., Makalowska I., Veeraraghavan N., Ward T. J., Zhang N., Kuldau G. A., O'donnell K., 2004 FUSARIUM-ID v. 1.0: A DNA Sequence Database for Identifying Fusarium. European. Journal of Plant Pathology 110 (5), 473-479
Google Scholar
Knutsen A. K., Torp M. and Holst-Jensen A., (2004) Phylogenetic analyses of the Fusarium poae, Fusarium sporotrichioides and Fusarium langsethiae species complex based on partial sequences of the translation elongation factor-1 alpha gene.
Google Scholar
International Journal of Food Microbiology 95 (3), 287-295
Google Scholar
Leslie J.F., Summerell B.A., 2006 The Fusarium Laboratory Manual. Blackwell, Oxford, UK.
Google Scholar
Nelson P.E., Tousson T.A. and Marasas W.F.O., 1983 Fusarium Species: An Illustrated Manual for Identification. The Pennsylvania State University Press.
Google Scholar
O’Donnell K., Kistler H.C., Cigelnik E. and Ploetz R.C., 1998 – Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mithocondrial gene genealogies. Proceeding of the National Academy of Sciences of the United States of America 95, 2044-2049.
Google Scholar
Orsini M., Romano-Spica V., 2001 A microwave-based method for nucleic acid isolation from environmental samples. Lett. Appl. Microbiol. 33, 17-20
Google Scholar
Pancaldi D., Grazzi G., Alberti I., 1995 – La fusariosi della spiga di frumento in Emilia-Romagna nel 1995. Informatore Fitopatologico 4, 16-20.
Google Scholar
Pancaldi D., Tonti S., Prodi A., Salamoni D., Dal Prà M., Nipoti P., Alberti I. and Pisi A., 2010 – Survey of the main causal agents of Fusarium Head Blight of durum wheat around Bologna, northern Italy. Phytopathologia Mediterranea 49, 260-268
Google Scholar
Parry D.W., Jenkinson P., Mac Leod L., 1995 Fusarium ear blight (scab) in small grains cereals – a review. Plant Pathology 44, 207-238.
Google Scholar
Parry D.W., Nicholson P., 1996 – Development of PCR assay to detect Fusarium poae in wheat. Plant Pathology 45, 383-391.
Google Scholar
Stenglein S.A., 2009 Fusarium poae: a pathogen that needs more attention. Journal of Plant Pathology 91 (1), 25-36.
Google Scholar
Stenglein S.A., Rodriguero M.S., Chandler E., Jennings P., Salerno G.L. and P. Nicholson, 2009 Phylogenetic relationships of Fusarium poae based on EF-1α and mtSSU sequences. Fungal Biology 114 (1), 96-106.
Google Scholar
Tamura K, Dudley J., Nei M. and Kumar S., 2007 MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24:1596-1599
Google Scholar
Torp M., Niremberg H.I., 2004 – Fusarium langsethiae sp.nov. on cereals in Europe. International Journal of Food Microbiology 95, 247-256.
Google Scholar
Vogelgsang, S., Sulyok, M., Hecker, A., Jenny, E., Krska, R., Schuhmacher, R., Forrer H.-R., 2008 Toxigenicity and pathogenicity of Fusarium poae and Fusarium avenaceum on wheat. European Journal of Plant Pathology 122, 265–276
Google Scholar
Xu X.M., Parry D., Nicholson P., Simpson D., Edwards S.G., Cooke B.M., Doohan F.M., Brennan J., Monaghan S., Moretti A., Tocco G., Mulè G., Hornok L., Giczey G. and Tantell J., 2005 Predominance and association of pathogenic species causing Fusarium ear blight in wheat. European Journal of Plant Pathology 112, 143-154.
Google Scholar
Xu X.M., Nicholson P., Thomsett M.A., Simpson D., Cooke B.M., Doohan F.M., Brennan J., Monaghan S., Moretti A., Mulè G., Hornok L., Beki E., Tantell J., Ritieni A. and Edwards S.G., 2008 Relationship between the fungal complex causing Fusarium Head Blight of wheat and environmental conditions. Phytopathology 98, 69-78.
Google Scholar
Autorzy
I. AlbertiENSE - Ente Nazionale Sementi Elette, Via Ca’ Nova Zampieri 37, S. Giovanni Lupatoto, Verona, Italy. Italy
Autorzy
Dal M. PràENSE - Ente Nazionale Sementi Elette, Via Ca’ Nova Zampieri 37, S. Giovanni Lupatoto, Verona, Italy. Italy
Autorzy
S. TontiENSE - Ente Nazionale Sementi Elette, Via Ca’ Nova Zampieri 37, S. Giovanni Lupatoto, Verona, Italy. Italy
Autorzy
M. MontanariENSE, Via Sicilia 2, Osteria Grande, Bologna, Italy. Italy
Autorzy
A. ProdiDepartment of Agroenvironmental Science and Technology (DiSTA) – Plant Pathology, Alma Mater Studiorum University of Bologna, Viale Fanin 40, Bologna, Italy. Italy
Autorzy
D. PancaldiDepartment of Agri-food Protection and Improvement (DIPROVAL), Alma Mater Studiorum University of Bologna, Viale Fanin 46, Bologna, Italy. Italy
Statystyki
Abstract views: 113PDF downloads: 68
Licencja
Wszystkie artykuły publikowane w formie elektronicznej na mocy licencji CC BY-SA 4.0, w otwartym dostępie (open access), pełna treść licencji jest dostępna pod adresem: https://creativecommons.org/licenses/by-sa/4.0/legalcode.pl .